Making chronic ischemia rat models by high-fat diet and thread embolism technique: evaluation and comparison of modeling methods

doi:10.3969/j.issn.2095-4344.1048

Abstract

Abstract:

BACKGROUND: Some studies have prepared a chronic ischemic animal model using the thread embolism technique, but the observation period is longer, and the survival time of the experimental animals may be shortened because of surgical trauma and disease. Herein, the authors further established a reliable animal model of chronic ischemia using the thread embolism technique by addition of high-fat diet.
OBJECTIVE: To investigate the feasibility of establishing a reliable animal model of chronic ischemia in high-fat diet rats using the thread embolism method.
METHODS: Forty Sprague-Dawley rats, 2 weeks of age, weighing 50-70 g, were provided by the Animal Experimental Center of Chengde Medical University in China. The rats were randomly divided into two groups, and fed with high-fat diet (experimental group) and normal diet (control group), respectively. Chronic ischemia animal models were then established in the hind limbs of all the rats using the thread embolism technique. Muscle strength of the rats was detected at 1, 2, 4, and 6 weeks postoperatively. Meanwhile, blood flow changes in the hind limbs and histological changes of the gastrocnemius and vascular tissue were observed using laser Doppler and hematoxylin-eosin staining, respectively.
RESULTS AND CONCLUSION: (1) Most of the rats in the experimental group had abnormal muscle performance at 1 week postoperatively. Most of the rats in the control group had nothing-unusual (P < 0.05). Most of the rats in both groups appeared with abnormal muscle performance at 2 and 4 weeks postoperatively, and there was no significant difference between the two groups. At 6 weeks postoperatively, the number of rats with abnormal muscle performance was lower in the control group than the experimental group. (2) Laser Doppler flow examination and histological examination both showed that the experimental group had ischemic symptoms earlier than the control group, and worse recovery from ischemia was found in the experimental group compared to the control group. These findings indicate that the thread embolism method can effectively make a rat model of chronic ischemia, and the addition of high-fat diet can cause an ischemic appearance earlier than the addition of normal diet, indicating the high-fat diet can prolong the state of chronic ischemia.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Models, Animal, Ischemia, Laser-Doppler Flowmetry, Tissue Engineering

CLC Number:

R446
R318

Chen Lei1, Ding Meng2, Zhang Qingyun1, Gao Jianguo1, Guo Fengling3, Yang Zhi1, Zhang Meng4 . Making chronic ischemia rat models by high-fat diet and thread embolism technique: evaluation and comparison of modeling methods[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(7): 1063-1067.

Figures/Tables 3

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