Polyglycolic acid-islet compound transplantation into rat leg muscles as diabetes treatment

doi:10.3969/j.issn.2095-4344.0984

Abstract

Abstract:

BACKGROUND: Islet transplantation is an effective method for diabetes treatment. Using biomedical engineering technology to introduce biomaterials into islet transplantation can maintain the vitality of islet and improve the efficiency of transplantation.

OBJECTIVE: To transplant the cultured polyglycolic acid (PGA)-islet into the muscle of diabetic recipient rats and to observe the effect of PGA-islet in the treatment of diabetes mellitus.

METHODS: A diabetic model was established in 18 Wistar rats by the method of intraperitoneal injection of streptozotocin, and the model rats were randomly assigned into three groups. In the control group, normal saline was injected into the muscle of the rat leg. In the experimental group 1, allogeneic islet cells were intramuscularly injected into the rat leg. In the experimental group 2, PGA-allogeneic islet cell graft was intramuscularly injected into the rat leg. At 3, 7, 14, 21 and 30 days after transplantation, blood glucose and serum insulin levels were detected in each rat. At 14 and 30 days after transplantation, graft specimens were taken from the experimental groups and observed by scanning electron microscopy.

RESULTS AND CONCLUSION: (1) At 3 and 7 days after transplantation, the blood glucose levels in the two experimental groups were significantly lower than that in the control group (P < 0.05), while the serum insulin levels in the two experimental groups were significantly higher than that in the control group (P < 0.05). Compared with the experimental group 1, the blood glucose level in the experimental group 2 was significantly lower at 14, 21 and 30 days after transplantation, while the serum insulin level in the experimental group 2 was significantly higher at each time point after transplantation (P < 0.05). Under the scanning electron microscope, in the experimental groups, plenty of well-formed islet cells were adhered to the PGA fiber scaffold at 14 days after transplantation; a visible network of extracellular matrix with fine fibers formed in islet cells; and a small number of red blood cells were visible around the islet cells. With the extension of embedded time, PGA fiber scaffolds began to degrade and crack. Long fibers degraded into short fibers, and after 30 days, the scaffold layer was broken into pieces finally. All these findings indicate that the transplantation of PGA-islet can effectively reduce the blood glucose level and increase the serum insulin level in diabetic rats.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Polyglycolic Acid, Diabetes Mellitus, Islets of Langerhans, Tissue Engineering

CLC Number:

R318

Xie Wan-jun, Wei Zheng, Song Chun. Polyglycolic acid-islet compound transplantation into rat leg muscles as diabetes treatment[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(30): 4829-4834.

Figures/Tables 4

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