Osteoclast activity of MG63 cells on silicon doped zirconia films prepared by cathodic arc deposition

doi:10.3969/j.issn.2095-4344.0738

Abstract

Abstract:

BACKGROUND: Various surface modification techniques have been utilized as an attempt to improve the osteointegration of Ti-based implants, which is a hotspot research concerning the artificial joint prosthesis.

OBJECTIVE: To evaluate the effect of silicon doped zirconia film on the titanium surface on relevant factors of osteoblast-like MG63 cells.

METHODS: Silicon doped zirconia and zirconia films were respectively prepared on the titanium surface by cathodic arc deposition. Osteoblast-like MG63 cells were cultured on silicon doped zirconia film, zirconia film and pure titanium, respectively. After 1, 4, 7, 10 days of incubation, samples were collected for assay. Osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL) at mRNA and protein levels were detected using real-time PCR and ELISA, respectively.

RESULTS AND CONCLUSION: (1) At 1 day of inoculation, there was no significant difference in the OPG mRNA and protein expression among the three groups. At 4 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group (P < 0.05), but there was no significant difference between the former two groups. At 7 and 10 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film growp was highest among the three groups (P < 0.05). (2) At 1 day of inoculation, there was no significant difference in the RANKL mRNA and protein expression among the three groups. At 4 and 7 days of inoculation, the expression of RANKL mRNA and protein in the silicon doped zirconia film group was significantly lower than that in the other two groups (P < 0.05). At 10 days of inoculation, the expression of OPG mRNA and protein in the silicon doped zirconia film and zirconia film groups was significantly higher than that in the pure titanium group (P < 0.05), but there was no significant difference between the former two groups. To conclude, silicon doped zirconia film fabricated by cathodic arc deposition can enhance the expression of OPG and reduce the expression of RANKL at the same time.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Titanium, Osteoblasts, Osteoprotegerin, Receptor Activator of Nuclear Factor-kappa B, Tissue Engineering

CLC Number:

R318

Zhang Shai-lin, Cheng Xiang-yu, Shi Ji-xiang, Zhou Qiang, Shi Wen-jun, Liu Fu-ying, Ji Bin. Osteoclast activity of MG63 cells on silicon doped zirconia films prepared by cathodic arc deposition[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(14): 2227-2232.

Figures/Tables 6

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