Effects of zinc ion contents on pro-osteogenic and antibacterial activities of micro-arc oxidation coatings

doi:10.3969/j.issn.2095-4344.0705

Abstract

Abstract:

BACKGROUND: Some studies have found that zinc ions have pro-osteogenic activity, while zinc ions at high levels are also reported to inhibit the differentiation of osteoblasts instead of the pro-osteogenic activity. In some studies, zinc ions that are injected into the surface of titanium have ineffective antibacterial effects. Therefore, further investigations on zinc ion’s effects as a controversial topic are required.

OBJECTIVE: To observe the effects of zinc ion content on the pro-osteogenic and antibacterial activities of micro-arc oxidation coatings.

METHODS: The coating was made on the surface of titanium by micro-arc oxidation method. The zinc ion content (atomic percentage) in the coating was set to 0.199%, 0.574%, and 1.995%, respectively, as low, medium and high dose groups. Untreated titanium plates were used as controls. MG63 cells were seeded on the surface of four groups of materials and tested for cell proliferation, morphological changes, and alkaline phosphatase activity. Staphylococcus aureus was inoculated on the surface of four groups of materials and the antibacterial rate was detected at 48 hours after inoculation. Bacterial adhesion was observed at 24 hours after inoculation.

RESULTS AND CONCLUSION: Within 7 days of culture, the number of MG63 cells on the material surface gradually increased with time. The proliferative ability of cells was highest in the low-dose group, followed by the middle-dose and control group, and it was lowest in the high-dose group. After 48 hours of culture, the cells in the control, low-dose and middle-dose groups showed normal morphology and expanded pseudopodia, and some pseudopodia penetrated into the cell surface, while normal or intact cells were undetected in the high-dose group. Within 13 days of culture, the activity of alkaline phosphatase was the highest in the low-dose group, followed by the middle-dose and control groups, and the lowest in the high-dose group. There were significant differences in the activity of alkaline phoshatase between groups (P < 0.05). The antibacterial rate of the materials was 62.54% in the low-dose group, 69.84% in the middle-dose group, and 79.19% in the high-dose group, respectively. Findings from this study reveal that with the increase of zinc ion contents, the pro-osteogenic activity of micro-arc oxidation coatings decreased, while the antibacterial property improved.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Titanium, Zinc, Tissue Engineering

CLC Number:

R318

Zhang Zhen-xiang. Effects of zinc ion contents on pro-osteogenic and antibacterial activities of micro-arc oxidation coatings[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(10): 1483-1487.

Figures/Tables 6

References

[1]porous silicon-incorporated TiO2 coating prepared by micro-arc oxidation.J Biomed Mater Res B Appl Biomater. 2011;97(2):224-234.

[2]Wang W,Wan P,Liu C,et al.Degradation and biological properties of Ca-P contained micro-arc oxidation self-sealing coating on pure magnesium for bone fixation.Regen Biomater. 2015;2(2):107- 118.

[3]Duarte LT,Bolfarini C,Biaggio SR.Growth of aluminum-free porous oxide layers on titanium and its alloys Ti-6Al-4V and Ti-6Al-7Nb by micro-arc oxidation.Mater Sci Eng C Mater Biol Appl.2014; 41:343-348.

[4]Yoshinari M,Oda Y,Kato T.Influence of surface modifications to titanium on antibacterial activity in vitro.Biomaterials. 2001; 22(14):2043-2048.

[5]Hu H,Zhang W,Qiao Y,et al.Antibacterial activity and increased bone marrow stem cell functions of Zn-incorporated TiO2 coatings on titanium.Acta Biomater.2012;8(2):904-915.

[6]Applerot G,Lipovsky A,Dror R,et al.Enhanced antibacterial activity of nanocrystalline ZnO due to increased ROS-mediated cell injury.Adv Funct Mater.2009;19:842-852.

[7]Storrie H,Stupp SI.Cellular response to zinc-containing organoapatite: an in vitro study of proliferation, alkaline phosphatase activity and biomineralization.Biomaterials. 2005;26:5492-5499.

[8]Tang A,Chappell HF,Dove MT.Zinc incorporation into hydroxylapatite.Biomaterials.2009;30: 2864-2872.

[9]Lusvardi G,Zaffe D,Menabue L,et al.In vitro and in vivo behaviour of zinc-doped phosphosilicate glasses.Acta Biomater. 2009;5:419-428.

[10]Miao S,Cheng K,Weng W,et al.Fabrication and evaluation of Zn containing ?uoridated hydroxyapatite layer with Zn release ability.Acta Biomater.2008;4:441-446.

[11]Zreiqat H,Ramaswamy Y,Wu C.The incorporation of strontium and zinc into a calcium–silicon ceramic for bone tissue engineering.Biomaterials.2010;31:3175-3184.

[12]Wu C,Ramaswamy Y,Chang J, et al.The Effect of Zn contents on phase composition, chemical stability and cellular bioactivity in Zn-Ca-Si system ceramics.J Biomed Mater Res B Appl Biomater. 2008;87:346-353.

[13]Ramaswamy Y,Wu C,Zhou H,et al.Biological response of human bone cells to zinc-modi?ed Ca–Si-based ceramics. Acta Biomater.2008;4:1487-1497.

[14]Tas AC,Bhaduri S,Jalota S.Preparation of Zn-doped b-tricalcium phosphate (β-Ca3(PO4)2) bioceramics. Mater Sci Eng C.2007;27:394-401.

[15]Xu J,Ding G,Li J,et al.Zinc-ion implanted and deposited titanium surfaces reduce adhesion of Streptococccus mutans. Appl Surf Sci.2010;256(24):7540-7544.

[16]Raghupathi KR,Koodali RT,Manna AC.Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles.Langmuir. 2011;27: 4020-4028.

[17]Reddy KM,Feris K,Bell J,et al.Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems.Appl Phys Lett.2007;90:2139021-2139023.

[18]Atmaca S,Gul K, Clcek R.The effect of zinc on microbial growth.Tr J Med Sci.1998;28:595-597.