Effect of salvianolic acid B on differentiation direction of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.0428

Abstract

Abstract:

BACKGROUND: With the development of tissue engineering, the multi-directional differentiation potential of bone marrow mesenchymal stem cells has brought a new hope for the treatment of many diseases. Salvianolic acid B, as an effective component of Salvia miltiorrhiza Bunge, has many pharmacological activities influencing the directional differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE: To summarize the effect of salvianolic acid B on the differentiation of bone marrow mesenchymal stem cells and to explore the underlying mechanisms.
METHODS: The first author searched the CNKI, WanFang and PubMed databases using the keywords of “salvianolic acid B, directional differentiation, mesenchymal stem cells” in Chinese and English, respectively, to retrieve relevant articles published from January 2000 to September 2017. One hundred and two Chinese articles and 136 English articles were initially retrieved. Repetitive articles or those with no originality were eliminated. Finally 49 articles were included in the result analysis.
RESULTS AND CONCLUSION: It has been found that salvianolic acid B can promote the osteogenic, neuronal and myocardial differentiation of bone marrow mesenchymal stem cells, but inhibit the adipogenic differentiation of the cells. Further investigations are required on whether salvianolic acid B can promote the mutli-directional differentiation of bone marrow mesenchymal stem cells and its effective concentrations.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Salvia miltiorrhiza, Bone Marrow, Mesenchymal Stem Cells, Cell Differentiation, Review, Tissue Engineering

CLC Number:

R394.2

Li Zi-yi, Wang Na, Xue Peng, Li Yu-kun. Effect of salvianolic acid B on differentiation direction of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(25): 4095-4100.

Figures/Tables 1

2.1 骨髓间充质干细胞骨髓间充质干细胞是处于静止状态的非造血干细胞，在受到外界刺激时可定向开启增殖分化程序[6]。近年来，随着医疗技术的发展，人们对骨髓间充质干细胞的认识越来越深入，分离、培养骨髓间充质干细胞并促进其分化的再生医学也越来越受到人们的重视，目前用于分离骨髓间充质干细胞的方法主要有密度梯度离心法和全骨髓培养法[7]。骨髓间充质干细胞具有重要的临床应用价值，不仅具有免疫调节能力，还可以利用其归巢特性运载特定的生物制剂治疗某些疾病。更为重要的是骨髓间充质干细胞也为组织再生提供了可能性，包括神经科、心血管科、骨科、血液科在内的多种临床科室都对骨髓间充质干细胞的研究表现出了浓厚的兴趣[8-11]。目前的研究结果显示，骨髓间充质干细胞的自身增殖能力强、分化范围广泛(图1[12])，调节骨髓间充质干细胞成脂/成骨分化比例可影响包括骨质疏松在内的多种骨科疾病的进程，诱导骨髓间充质干细胞向心肌细胞定向分化可治疗多种心血管疾病，而诱导骨髓间充质干细胞定向分化为神经细胞可治疗多种神经系统疾病[13-15]。因此，研究和关注骨髓间充质干细胞的多向分化具有重要意义，可为多种疾病的治疗带来新的思路。 2.2 丹酚酸B的药理特性及应用丹酚酸B是中药丹参中最为重要的活性成分，其基本结构为四聚体的咖啡酸衍生物，可溶于水、甲醇、乙醇等发挥其药理作用[16-18]。研究表明，丹酚酸B可清除自由基、抗氧化、改善血流、抑制血小板聚集和形成、抗纤维化、抑制细胞凋亡，在多种疾病的治疗中具有重要的作用。在心、脑血管疾病中，丹酚酸B可通过Akt-eNOS信号、TLR4-NFκB-TNF-α炎症通路等多种信号通路抑制心肌细胞肥大、抑制动脉粥样硬化形成[19-20]。在肾脏疾病中，丹酚酸B可通过 PI3K/Akt、TGF-β1/Smads、Wnt/β-catenin等信号通路抑制肾间质纤维化[21-23]。目前，包括丹参注射液在内的多种包含丹酚酸B的制剂在临床中广泛应用。近年来，丹酚酸B影响骨髓间充质干细胞生长、分化的研究也逐渐深入，为其应用开拓了新的方向(表1)。"

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