Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (12): 1872-1876.doi: 10.3969/j.issn.2095-4344.0205

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Correlation of serum C-telopeptide cross-linked collagen type I with bone mineral density in a rat model of disuse osteoporosis

Yi Kai-jun, Wang Lian-peng, Ding Hui-yong, Kong Dan, Han Ya-jun, Ma Feng-hua, Duan Wei-nan, Zhou Gao-jin, Li Guo-hua   

  1. Department of Orthopedics, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • Received:2018-02-24 Online:2018-04-28 Published:2018-04-28
  • Contact: Li Guo-hua, Master, Associate professor, Chief physician, Department of Orthopedics, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • About author:Yi Kai-jun, Master candidate, Physician, Department of Orthopedics, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2015211C102

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Abstract:

BACKGROUND: C-telopeptide and N-telopeptide cross-linked collagen type I (CTx and NTx, respectively) are specific biochemical bone markers that can reflect bone formation and resorption.
OBJECTIVE: To analyze the association of CTx with disuse osteoporosis.
METHODS: Male Sprague-Dawley rats, weighing 180-220 g, were randomly divided into control and disuse osteoporosis groups. Right hind limbs of the rats in the disuse osteoporosis group were immobilitzed for 4 weeks by ankle-tail fixation to establish the rat model of disuse osteoporosis. Peritoneal venous blood was collected before and after modeling, and the femur was then removed to measure the serum CTx level and bone mineral density of the bilateral femurs.
RESULTS AND CONCLUSION: The serum CTx level did not differ significantly between groups before modeling (P > 0.05). At 4 weeks after modeling, the serum CTx level in the disuse osteoporosis group was significantly higher than that in the control group and at baseline (P < 0.01). The serum CTx level showed no significant change in the control group before and after modeling (P > 0.05). The increment of serum CTx in the disuse osteoporosis group exhibited a negative correlation with the bone mineral density of the bilateral femurs (r=0.426, P < 0.01). The bone mineral density of the right femur in the disuse osteoporosis group was significantly lower than that of the left one in the disuse osteoporosis group and the right one in the control group (P < 0.01), and there was no significant difference in the bone mineral density between left and right femurs in the control group (P > 0.05). These results imply that the model of disuse osteoporosis by ankle-tail fixation is established successfully. Disuse osteoporosis can promote the production of CTx further reducing bone mineral density; CTx is positively correlated with the degree of bone loss, so it can be used for therapeutic assessment and diagnosis of osteoporosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoporosis, Peptides, Bone Density, Tissue Engineering

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