Indirect co-culture with chondrocytes induces periosteum-derived cells to differentiate into chondrocytes

doi:10.3969/j.issn.2095-4344.0088

Abstract

Abstract:

BACKGROUND: Under indirect co-culture conditions, chondrocytes can induce bone marrow mesenchymal stem cells to differentiate into chondrocytes. Osteocytes are the seed cells of chondrocytes, but co-culture of osteocytes with chondrocytes is rarely reported.
OBJECTIVE: To induce the chondrogenic differentiation of periosteum-derived cells (PDCs) by indirect co-culture with chondrocytes.
METHODS: Chondrocytes were isolated from the rabbits by trypsin and collagenase II digestion. Rabbit PDCs were obtained by the explants culture method. Passage 2 chondrocytes and PDCs underwent indirect co-culture at 1:1 in Transwell system as experimental group. PDCs cultured alone were as control group. After 2 weeks of culture, the cellular morphological changes were observed by inverted contrast phase microscope. The expression levels of collagen type II and proteoglycan were detected by collagen type II immunohistochemistry and toluidine blue staining. The mRNA expression levels of proteoglycan, collagen type II and SRY-related protein-9 were detected by RT-PCR.
RESULTS AND CONCLUSION: After 2 weeks of culture, PDCs in the experimental group were gradually transformed to chondrocyte-like cells, while PDCs in the control group still remained long spindle-shaped. Collagen type II immunohistochemistry staining and toluidine blue staining were positive in the experimental group, while the results were negative in the control group. Additionally, RT-PCR results indicated that the relative mRNA expression levels of proteoglycan, collagen type II and SRY-related protein-9 in the experimental group were significantly higher than those in the control group. Therefore, chondrocytes can induce PDCs to differentiate into chondrocytes under indirect co-culture conditions.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Chondrocytes, Periosteum, Cell Differentiation, Collagen Type II, Proteoglycans, Tissue Engineering

CLC Number:

R318

Li Wei1, Yuan Feng2, Wo Jin1, Zhang Teng1, Gao Peng1. Indirect co-culture with chondrocytes induces periosteum-derived cells to differentiate into chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(4): 548-552.

Figures/Tables 2

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