Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (28): 4477-4483.doi: 10.12307/2024.395
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Jiang Yu1, Xu Lin1, Zhao Yalin2, Liu Gang1, Zhang Yaqi1, Bai Huizhong1, Ren Jingpei1, Zeng Jie3, Mu Xiaohong1
Received:
2023-05-30
Accepted:
2023-07-04
Online:
2024-10-08
Published:
2023-11-27
Contact:
Mu Xiaohong, MD, Professor, Chief physician, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100007, China
About author:
Jiang Yu, Master candidate, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100007, China
Supported by:
CLC Number:
Jiang Yu, Xu Lin, Zhao Yalin, Liu Gang, Zhang Yaqi, Bai Huizhong, Ren Jingpei, Zeng Jie, Mu Xiaohong. Effects of Shujin Jiannao Prescription on cell apoptosis in rats with hypoxic-ischemic brain injury[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(28): 4477-4483.
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2.5.1 Bcl-2的表达 结果显示,模型组Bcl-2表达最少,舒筋健脑方高剂量组Bcl-2表达最多,舒筋健脑方各剂量组Bcl-2表达量随药物剂量增加而增加;与模型组相比,舒筋健脑方中、高剂量组Bcl-2表达显著增加(P < 0.05);与米诺环素组相比,舒筋健脑方高剂量Bcl-2表达显著增加(P < 0.05)。 2.5.2 Bax的表达 结果显示,模型组Bax表达最多,舒筋健脑方中剂量组Bax表达最少;与模型组相比,米诺环素组、舒筋健脑方低、中和高剂量组Bax表达显著减少(P < 0.05);与米诺环素组相比,舒筋健脑方低剂量Bax表达显著增加,舒筋健脑方中剂量Bax表达显著减少(P < 0.05)。 2.5.3 Caspase-3的表达 结果显示,舒筋健脑方低剂量组Caspase-3表达最多,舒筋健脑方高剂量组Caspase-3表达最少,舒筋健脑方各剂量组表达量随药物剂量增加而减少;与模型组相比,米诺环素组、舒筋健脑方各剂量组的Caspase-3表达均有显著性意义(P < 0.05);与米诺环素组相比,舒筋健脑方低剂量组Caspase-3表达显著增加(P < 0.05)。 2.6 Western blot检测各组大鼠海马CA1区Bcl-2、Bax、Caspase-3蛋白的表达 见图5,表6。"
2.6.1 Bcl-2蛋白表达 结果显示,模型组表达少,对照组表达最多,舒筋健脑方各剂量组蛋白的表达量随着药物的剂量成正比;与模型组相比,舒筋健脑方高剂量组蛋白表达显著减少(P < 0.05)。 2.6.2 Bax蛋白表达 结果显示,模型组表达最多,对照组表达最少;与模型组相比,米诺环素组、舒筋健脑方各剂量组表达显著减少(P < 0.05)。 2.6.3 Bcl-2/Bax的比值 模型组Bcl-2/Bax的比值显著低于其他各组(P < 0.05),其中舒筋健脑方各剂量组的比值与药物的剂量成正比。 2.6.4 Caspase-3蛋白表达 结果显示,舒筋健脑方低剂量组表达最多,对照组表达最少;与模型组相比,舒筋健脑方高剂量组蛋白表达显著减少(P < 0.05);与米诺环素组相比,舒筋健脑方低剂量组蛋白表达显著增加(P < 0.05)。"
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