Differential effects of hypoxia and oxidative stress on paracrine of mesenchymal stem cells from different sources

doi:10.12307/2024.140

Abstract

Abstract: BACKGROUND: The biological behavior of mesenchymal stem cells is influenced by the survival microenvironment. Pre-treatment of the microenvironment is an important means of regulating the function of mesenchymal stem cells.
OBJECTIVE: To compare the differences in paracrine function of umbilical cord mesenchymal stem cells and adipose mesenchymal stem cells under oxidative stress and hypoxia, and to provide a theoretical basis for selecting appropriate pretreatment of mesenchymal stem cells to treat different diseases.
METHODS: Umbilical cord mesenchymal stem cells and adipose mesenchymal stem cells were cultured by H2O2 or O2 oxygen, respectively, and cell morphology, proliferation, viability and paracrine factor expression were examined.
RESULTS AND CONCLUSION: (1) The expression levels of brain-derived neurotrophic factor and transforming growth factor-β were higher in umbilical cord mesenchymal stem cells than in adipose mesenchymal stem cells under a normal culture environment, while the expressions of stromal cell-derived factor-1α and tumor necrosis factor stimulating factor-6 in the adipose mesenchymal stem cells were significantly higher than those in the umbilical cord mesenchymal stem cells. (2) There was no significant difference in the effect of low and moderate levels (≤ 100 μmol/L) of H2O2 on the viability of the two mesenchymal stem cells. However, increasing the H2O2 concentration from 50 μmol/L to 100 μmol/L resulted in a distinct increase in vascular endothelial growth factor expression in umbilical cord mesenchymal stem cells. The expression of basic fibroblast growth factor, vascular endothelial growth factor, stromal cell-derived factor-1α and interleukin-10 in adipose mesenchymal stem cells was greatly increased by increasing H2O2 concentration in this range. (3) 1% O2 hypoxia promoted mesenchymal stem cell proliferation. After 24 hours of culture in 1% O2, gene expression levels were elevated in both mesenchymal stem cells, but the expression levels of vascular endothelial growth factor, interleukin-10 and tumor necrosis factor stimulating factor-6 were significantly higher in adipose mesenchymal stem cells than in umbilical cord mesenchymal stem cells. (4) It is concluded that hypoxia and oxidative stress preconditioning enhances the paracrine function of mesenchymal stem cells. However, mesenchymal stem cells respond differently to hypoxia and oxidative stress. Treating diseases can choose suitable mesenchymal stem cells for appropriate pretreatment to further enhance their therapeutic potential.

Key words: umbilical cord mesenchymal stem cell, adipose mesenchymal stem cell, paracrine, oxidative stress, hypoxia

CLC Number:

Pan Xiaoying, Xu Yongde, Liu Zhiqiang, Xing Xiaowen, Yang Yong. Differential effects of hypoxia and oxidative stress on paracrine of mesenchymal stem cells from different sources[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(19): 3024-3030.

Figures/Tables 8

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