Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (21): 3438-3444.doi: 10.12307/2024.068
Xie Yue1, 2, Zhai Weifeng2, Guo Ji2, Jia Yongwei2
Received:
2023-03-08
Accepted:
2023-04-24
Online:
2024-07-28
Published:
2023-09-28
Contact:
Jia Yongwei, MD, Chief physician, Doctoral supervisor, Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
About author:
Xie Yue, Master candidate, Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China; Guanghua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Supported by:
CLC Number:
Xie Yue, Zhai Weifeng, Guo Ji, Jia Yongwei. Integrative analyses of pyroptosis-related genes associated with intervertebral disc degeneration[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(21): 3438-3444.
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2.6 椎间盘退变关键生物标志物的获取及鉴定 利用GSE150408数据集对上述筛选到的椎间盘退变差异表达的细胞焦亡基因进行验证。使用limma包进行差异分析,共得到了1 584个差异表达的基因,其中包括927个上调基因和657个下调基因,利用差异表达基因和数据分组绘制了火山图热图(图6A,B)。将GSE150408和GSE124272两组数据集差异表达的基因与细胞焦亡基因取交集后(图6C),得到1个差异表达的细胞焦亡相关基因——NLRP3,通过GSE150408验证结果发现,该基因在椎间盘退变患者中高表达(图6D)。同时,将筛选出来的关键细胞焦亡相关基因在2个数据集中进行了ROC分析并计算了曲线下面积。结果显示2个数据集中曲线下面积值均大于0.6,表明NLRP3基因能准确区分椎间盘退变患者和健康对照,具有潜在的诊断价值(图7)。"
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