Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (14): 2159-2165.doi: 10.12307/2024.329
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Yuan Tianyi1, Liu Hongjiang1, Yang Zengqiang1, Lu Xingbao1, Maimaitiyibubaji1, Zhou Zhiheng1, Cui Yong1, 2
Received:
2023-04-12
Accepted:
2023-05-25
Online:
2024-05-18
Published:
2023-07-28
Contact:
Cui Yong, Chief physician, Associate professor, Master’s supervisor, The Fifth Clinical Medical College of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Department of Orthopedics, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
About author:
Yuan Tianyi, Master candidate, The Fifth Clinical Medical College of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
Supported by:
CLC Number:
Yuan Tianyi, Liu Hongjiang, Yang Zengqiang, Lu Xingbao, Maimaitiyibubaji, Zhou Zhiheng, Cui Yong. Identification of differences in N6-methyladenosine-related genes in steroid-induced femoral head necrosis[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(14): 2159-2165.
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2.3 m6A相关调控因子在GSE123568数据集和GSE74089数据集中差异表达分析结果 将m6A调控因子在GSE123568数据集中激素股骨头坏死和非激素股骨头坏死表达分析发现,其中METTL3、IGF2BP3、WTAP、CBLL1、KIA1429、HNRNPA2B1、FMR1、YTHDF2、ALKBH5、YTHDF1表达差异(P < 0.05),YTHDF3、YTHDC2、IGF2BP2表达差异(P < 0.01);IGF2BP3、IGF2BP2、ALKBH5在激素股骨头坏死组中为下调性表达,YTHDF3、YTHDC2、METTL3、WTAP、CBLL1、KIA1429、HNRNPA2B1、FMR1、YTHDF2、YTHDF1在激素股骨头坏死组中为上调性表达,见图4A。m6A调控因子在GSE74089数据集股骨头坏死组与对照组软骨组织中表达分析发现,YTHDF2在股骨头坏死组中为高表达,并且差异有显著性意义(P < 0.05),图4B。将m6A调控因子在GSE123568数据集进行受试者工作特征曲线分析发现,其中YTHDF3、YTHDC2、IGF2BP3、CBLL1、KIA1429、IGF2BP2、HNRNPA2B1、YTHDF2中的受试者工作特征AUC均> 0.75,因此认为其可以作为激素性股骨头坏死的生物标志物,见图5。"
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