Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (14): 2180-2185.doi: 10.12307/2024.296
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Xie Wenguan, Liu Yutao, Cui Wenbo, Kuang Mingye
Received:
2023-04-04
Accepted:
2023-05-08
Online:
2024-05-18
Published:
2023-07-28
Contact:
Kuang Mingye, Associate chief physician, Department of Orthopedics, Dongguan Eighth People’s Hospital, Dongguan Children’s Hospital Affiliated to Guangdong Medical University, Dongguan 523320, Guangdong Province, China
About author:
Xie Wenguan, Master, Department of Orthopedics, Dongguan Eighth People’s Hospital, Dongguan Children’s Hospital Affiliated to Guangdong Medical University, Dongguan 523320, Guangdong Province, China
Supported by:
CLC Number:
Xie Wenguan, Liu Yutao, Cui Wenbo, Kuang Mingye. Melatonin inhibits hydrogen peroxide-induced injury of human nucleus pulposus cells[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(14): 2180-2185.
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2.4 不同浓度褪黑素对过氧化氢处理人髓核细胞增殖的影响 图4为经过氧化氢处理的人髓核细胞在不同浓度褪黑素干预下的增殖情况,结果表明,在褪黑素干预24 h后,人髓核细胞的抗氧化作用表现出不同程度提升,且呈浓度依赖性。当过氧化氢干预浓度为400 μmol/L时,单纯模型组的人髓核细胞存活率为(71.00±3.37)%,而当褪黑素干预浓度为1 μmol/L时,人髓核细胞存活率则为(78.84±2.59)%,差异有显著性意义(P=0.020 < 0.05)。当褪黑素干预浓度为5 μmol/L时,人髓核细胞存活率则为(86.93±2.48)%,差异有非常显著性意义(P=0.000 < 0.01),说明褪黑素能减轻过氧化氢对人髓核细胞的氧化作用,增强对人髓核细胞的保护作用,最佳抗氧化浓度为5 μmol/L。"
2.7 褪黑素抑制Bax、caspase3基因的表达,促进Bcl-2基因的表达 Bax、caspase3基因的高表达可以促进细胞凋亡。如图7所示,模型组人髓核细胞中Bax和Caspase3的mRNA表达水平均显著高于对照组(P=0.000,0.000 < 0.01),Bcl-2的mRNA表达水平显著低于对照组(P=0.000 < 0.01)。与模型组相比,褪黑素组Bax和Caspase3的mRNA表达水平显著降低(P=0.006,0.003 < 0.01)。此外,与模型组相比,褪黑素组Bcl-2的mRNA表达水平升高(P=0.015 < 0.05)。因此,褪黑素可以抑制过氧化氢诱导的人髓核细胞凋亡。"
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