Preparation of calcium silicate microspheres loaded with epigallocatechin gallate and investigation on its antibacterial performance

doi:10.12307/2023.553

Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (30): 4769-4775.doi: 10.12307/2023.553

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Preparation of calcium silicate microspheres loaded with epigallocatechin gallate and investigation on its antibacterial performance

Fan Haomei1, 2, 3, Xiao Dongqin2, Shi Feng4, Luo Xuwei2, Wei Jianlin3, Zhuang Huadi3, Liu Jinhui5, Zhao Juhua1, 3

1School of Clinical Medicine, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Second Clinical College of North Sichuan Medical College·Tissue Engineering and Stem Cell Research Institute of Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China; 3Department of Dermatology of Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China; 4Collaborative Innovation Center for Tissue Repair Material Engineering Technology, China West Normal University, Nanchong 637000, Sichuan Province, China; 5Department of Orthopedics and Arthrology, Affiliated Hospital of Southwest Medical University, Sichuan Province Orthopaedic Implantation Device Development and Application Technology Engineering Laboratory, Luzhou 646000, Sichuan Province, China

Received:2022-08-05 Accepted:2022-10-14 Online:2023-10-28 Published:2023-04-01
Contact: Xiao Dongqin, PhD, Associate researcher, Second Clinical College of North Sichuan Medical College·Tissue Engineering and Stem Cell Research Institute of Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China Liu Jinhui, Engineer, Department of Orthopedics and Arthrology, Affiliated Hospital of Southwest Medical University, Sichuan Province Orthopaedic Implantation Device Development and Application Technology Engineering Laboratory, Luzhou 646000, Sichuan Province, China Zhao Juhua, PhD, Chief physician, School of Clinical Medicine, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Department of Dermatology of Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China
About author:Fan Haomei, Master candidate, School of Clinical Medicine, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Second Clinical College of North Sichuan Medical College·Tissue Engineering and Stem Cell Research Institute of Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China; Department of Dermatology of Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China
Supported by:
National Natural Science Foundation of China, No. 82002289 (to XDQ); Sichuan Provincial Department of Science and Technology Applied Basic Research Project, No. 2022NSFSC0685 (to XDQ), No. 2022NSFSC0609 (to LXW); Sichuan Provincial Medical Research Project Program, No. S20012 (to XDQ); Nanchong City School Cooperation, No. 20SXQT0335 (to XDQ), No. 18SXHZ03590 (to ZJH); Medical Science and Technology Project of Sichuan Provincial Health Commission, No. 21PJ196 (to LXW), No. 21PJ197 (to ZJH); Luzhou City Key Research & Development Technology Plan Project of Luzhou City Science and Technology and Talent Work Bureau, No. 2018-GYF-10 (to LJH); Sichuan Provincial Administration of Traditional Chinese Medicine Special Project on Traditional Chinese Medicine, No. 2020LC0149 (to ZJH)

Abstract

Abstract: BACKGROUND: The main active component of tea polyphenols is epigallocatechin gallate, which has various biological functions such as antioxidation, antibacteria, anti-apoptosis, and anti-inflammation. To realize the controllable release of tea polyphenols and improve their bioavailability, it is urgent to develop bioactive carriers for tea polyphenols.
OBJECTIVE: To prepare microspheres loaded with high-efficiency epigallocatechin gallate, and measure its antibacterial performance and biocompatibility.
METHODS: Calcium silicate microspheres were prepared by chemical precipitation method. X-ray photoelectron spectroscopy, transmission electron microscopy, field emission scanning electron microscopy, surface area measurement and laser particle size analyzer were used to characterize the performance of the microspheres. The two kinds of microspheres were immersed in epigallocatechin gallate solution respectively to prepare calcium silicate microspheres and silica microspheres loaded with epigallocatechin gallate (named as CSM-EGCG and SM-EGCG, respectively). The drug loading rate and encapsulation rate of the microspheres, as well as the release of epigallocatechin gallate in vitro were detected. The unloaded calcium silicate microspheres, silica microspheres, CSM-EGCG and SM-EGCG were co-cultured with Staphylococcus aureus and Escherichia coli, respectively, to detect the antibacterial capacity. The above four kinds of microspheres were co-cultured with human skin fibroblasts respectively, and the cell viability was evaluated by CCK-8 assay.
RESULTS AND CONCLUSION: (1) The characterization experiment results showed that the prepared calcium silicate spheres and silica spheres were mesoporous microspheres, and the specific surface area, total pore volume and average pore size of calcium silicate spheres were larger than those of silica spheres. (2) The encapsulation rate and drug loading rate of CSM-EGCG were (72.0±0.5)% and (58.4±0.4)%, respectively. The encapsulation rate and drug loading rate of SM-EGCG were (41.6±0.7)% and (45.0±1.3)%, respectively. CSM-EGCG microspheres had better drug loading capacity than SM-EGCG microspheres, and the drug release time in vitro lasted for more than 19 days, and the cumulative release reached (88.1±3.0)%. (3) Silica microspheres had no antibacterial activity. The antibacterial rates of calcium silicate microspheres, SM-EGCG, and CSM-EGCG against Staphylococcus aureus were (28.0±4.2)%, (63.9±1.0)%, and (95.6±0.5)%, respectively. The antibacterial rates against Escherichia coli were (27.5±7.0)%, (51.9±1.4)%, and (93.4±1.0)%, respectively. (4) CCK-8 assay results showed that the calcium silicate spheres and CSM-EGCG had good cell biocompatibility and showed enhanced proliferation of human skin fibroblasts. (5) These results verified that the epigallocatechin gallate-loaded calcium silicate spheres have good antibacterial activity and cytocompatibility.

Key words: calcium silicate, tea polyphenols, sustained release, antibacterial property, epigallocatechin gallate, biocompatibility

CLC Number:

Fan Haomei, Xiao Dongqin, Shi Feng, Luo Xuwei, Wei Jianlin, Zhuang Huadi, Liu Jinhui, Zhao Juhua. Preparation of calcium silicate microspheres loaded with epigallocatechin gallate and investigation on its antibacterial performance[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(30): 4769-4775.

Figures/Tables 11

References

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Preparation of calcium silicate microspheres loaded with epigallocatechin gallate and investigation on its antibacterial performance

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