Preparation of hierarchical porous cartilage composite scaffolds loaded with stromal cell-derived factor-1 using low-temperature deposition 3D printing

doi:10.12307/2023.543

Abstract

Abstract: BACKGROUND: The treatment regimen based on the promotion of stem cell recruitment to achieve in situ regeneration and repair of cartilage damage is a new strategy in cartilage tissue engineering.
OBJECTIVE: To prepare hierarchical porous cartilage composite scaffolds loaded with stromal cell-derived factor-1 (SDF-1) using low-temperature deposition 3D printing.
METHODS: The cartilage was decellularized by a chemical method. The bioink was prepared with the addition of SDF-1 to decellularized cartilage extracellular matrix homogenate. The graded porous extracellular matrix scaffolds were constructed using low-temperature deposition 3D printing. The microstructure of scaffolds was observed by scanning electron microscopy. The biocompatibility of the scaffolds was evaluated by CCK-8, live-dead cell staining, and ghost pen peptide cell skeleton staining. The in vitro sustained release of the scaffolds was tested, and the effects of the scaffolds on the migration of bone marrow mesenchymal stem cells were detected by Transwell assay.
RESULTS AND CONCLUSION: (1) Scanning electron microscopy showed that the printed extracellular matrix scaffolds had a hierarchical porous structure, with fibers staggered and interconnected between pores. (2) The CCK-8 results indicated that the prepared extracellular matrix scaffolds had no obvious cytotoxicity. Live-dead cell staining showed that bone marrow mesenchymal stem cells were able to grow well on the extracellular matrix scaffolds; ghost pen peptide cell skeleton staining showed that bone marrow mesenchymal stem cells were able to spread well on the extracellular matrix scaffold in a shuttle shape. (3) The extracellular matrix scaffold had a good sustained release property. SDF-1 was released rapidly during the initial 7 days, with a cumulative release rate of about 60%. The release rate of SDF-1 gradually slowed down during 1-4 weeks, and the release pattern during 4-6 weeks was similar to the zero-order release kinetics, and the cumulative release rate reached about 80% at 42 days, and there was still a trend of slow release. (4) The results of Transwell migration assay showed that SDF-1 improved the ability of extracellular matrix scaffolds to recruit stem cells. (5) These findings suggest that SDF-1 can improve the ability of low-temperature deposited 3D printed extracellular matrix scaffolds to recruit bone marrow mesenchymal stem cells.

Key words: extracellular matrix, scaffold, stromal cell-derived factor-1, low-temperature deposition 3D printing, cartilage injury, bone marrow mesenchymal stem cell, migration

CLC Number:

Wu Jiang, Yin Han, Yan Zineng, Tian Guangzhao, Ding Zhengang, Yuan Xun, Fu Liwei, Tian Zhuang, Sui Xiang, Liu Shuyun, Guo Quanyi. Preparation of hierarchical porous cartilage composite scaffolds loaded with stromal cell-derived factor-1 using low-temperature deposition 3D printing[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(30): 4776-4782.

Figures/Tables 9

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