Antibacterial and hemostatic properties of chitosan collagen sponge

doi:10.12307/2023.463

Abstract

Abstract: BACKGROUND: Collagen, which has good biocompatibility and degradability, is commonly used to promote wound healing, but it is not effective in preventing wound infection because of its non-antibacterial properties.
OBJECTIVE: To prepare chitosan collagen sponge by freeze-drying method, which has good antibacterial and hemostatic effects.
METHODS: Chitosan solution and sodium bicarbonate solution became gel at 37 ℃. Chitosan/sodium bicarbonate/type I collagen composite sponge was prepared by freeze-drying method as the experimental group, and chitosan sponge and chitosan/sodium bicarbonate sponge were used as the control group. Scanning electron microscopy was used to observe the micro-morphology of the sponge and determine its pH value, porosity, water absorption, degradation rate and antimicrobial activity against Staphylococcus aureus and Escherichia coli. The cytotoxicity of the sponges was detected by CCK-8 assay and Live/Dead staining. The hemostatic effect of the sponges was detected by the rat model of amputation bleeding. The rat model of skin trauma was established. The tissue healing was observed by hematoxylin-eosin staining and Masson staining 7 days after sponge implantation.
RESULTS AND CONCLUSION: (1) Compared with the other sponge groups, the chitosan/sodium bicarbonate/type I collagen composite sponge had larger porosity, rougher outer wall, stronger water absorption, greater porosity and water absorption multiple, and the porosity was greater than the other sponge groups (P < 0.01). The average water absorption multiple was greater than chitosan sponge (P < 0.000 1) and chitosan/sodium bicarbonate sponge (P < 0.05). (2) Anti-Escherichia coli test results showed that Escherichia coli resistance rate was chitosan sponge > chitosan/sodium bicarbonate sponge (P < 0.001) > chitosan/sodium bicarbonate/type I collagen composite sponge (P < 0.000 1). (3) Anti-Staphylococcus aureus rate test results showed that anti-Staphylococcus aureus rate was chitosan sponge > chitosan/sodium bicarbonate sponge > chitosan/sodium bicarbonate/type I collagen composite sponge (P < 0.01). (4) Chitosan/sodium bicarbonate/type I collagen composite sponge was non-cytotoxic and could promote the proliferation of mouse fibroblasts (L929), and the pro-proliferation effect was better than that of the blank group (P < 0.05), the chitosan sponge group (P < 0.000 1) and the chitosan/sodium bicarbonate sponge group (P < 0.05). (5) Chitosan/sodium bicarbonate/type I collagen composite sponge had a good hemostasis effect, which was better than the other three groups. (6) Chitosan/sodium bicarbonate/type I collagen composite sponge was implanted into the wound on the back of rats for one week without obvious inflammation in the surrounding skin, and the contents of capillary and collagen were significantly higher than those of chitosan sponge and chitosan/sodium bicarbonate sponge. The skin tissue recovered best. (7) It is concluded that chitosan/sodium bicarbonate/type I collagen composite sponge has excellent biocompatibility, antibacterial hemostasis and tissue healing properties.

Key words: chitosan, type I collagen, sodium bicarbonate, hemostatic material, antibacterial, tissue repair, hemostatic sponge, wound healing

CLC Number:

Zhu Hong, Lin Ziheng, He Rouye, Pan Jinbin, Liu Xiaochuan, He Xiaoling, Zhang Jingying. Antibacterial and hemostatic properties of chitosan collagen sponge[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(16): 2525-2533.

Figures/Tables 12

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