Ecto-mesenchymal stem cell-conditioned medium lyophilized powder combined with fibrin glue to repair skin injury in rats

doi:10.12307/2023.386

Abstract

Abstract: BACKGROUND: Recently, most studies have combined tissue engineering materials with stem cells or factors to improve animal skin injury models. Ecto-mesenchymal stem cells derived from nasal mucosa can be preserved for a long time and has unique advantages in repairing skin damage.
OBJECTIVE: To observe the effect of ecto-mesenchymal stem cells-conditioned medium lyophilized powder combined with fibrin glue on the wound healing of skin defects in rats.
METHODS: Ecto-mesenchymal stem cells derived from Sprague-Dawley rat nasal mucosa were cultured in vitro, and the conditioned medium from passage 3 was collected and lyophilized. The 30 rat models of skin defects were divided into three groups according to treatment (n=10): the injury control group, received no specific treatment as control; the fibrin glue group, received topical application of fibrin glue; the combination group, received topical application of the fibrin glue contained ecto-mesenchymal stem cells-conditioned medium lyophilized powder. After treatments, the wound healing was observed dynamically and the wound area was measured. The tissue sections of the skin were stained with hematoxylin and eosin and subjected to immunohistochemical staining of cytokeratin-8 and protein-63.
RESULTS AND CONCLUSION: The wound healing rate of the combination group was higher than that of the fibrin glue group and the injury control group (P < 0.001). The staining of tissue sections showed that the epidermis and dermis of the combination group were all regenerated and repaired in 21 days; the regenerated skin in the fibrin glue group was thinner and the skin healing in the injury control group was incomplete. Ecto-mesenchymal stem cells-conditioned medium lyophilized powder in combination with fibrin glue can enhance wound healing of the injured skin in rat models.

Key words: skin injury, nasal mucosa, ecto-mesenchymal stem cell, conditioned medium, fibrin glue, wound healing

CLC Number:

Chen Di, Xue Yu, Tang Yu, Fei Xiaoming, Zhuang Qin, Zhou Wenwen, Lyu Demin, Shi Wentao, Zhang Zhijian, Zheng Wenjuan, Jiang Yu. Ecto-mesenchymal stem cell-conditioned medium lyophilized powder combined with fibrin glue to repair skin injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(15): 2350-2355.

Figures/Tables 4

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