Echinococcus granulosus promotes the proliferation of bone marrow mesenchymal stem cells

doi:10.12307/2022.378

Abstract

Abstract: BACKGROUND: At present, there are few reports on the interaction between Echinococcus granulosus and mesenchymal stem cells at home and abroad, and the research on Echinococcus granulosus and stem cell proliferation is still blank.
OBJECTIVE: To explore the effect and mechanism of Echinococcus granulosus on the proliferation of bone marrow mesenchymal stem cells.
METHODS: C57BL/6 mouse bone marrow mesenchymal stem cells were isolated and cultured, and divided into bone marrow mesenchymal stem cell group, Notch signaling pathway specific inhibitor DAPT group, Echinococcus granulosus protocercaria group, and Echinococcus granulosus protocercaria + specific inhibitor DAPT group. At 1, 2, and 3 days after culture, CCK-8 assay was used to detect the proliferation of bone marrow mesenchymal stem cells in each group. Western blot assay and real-time fluorescent quantitative PCR were used to detect expression levels of Notch-1, Jagged-1, Hes-1 proteins and mRNA related to the Notch signaling pathway in bone marrow mesenchymal stem cells of each group.
RESULTS AND CONCLUSION: (1) Compared with bone marrow mesenchymal stem cell group, and the proliferation was inhibited in the specific inhibitor DAPT group (P < 0.05), and the proliferation of the Echinococcus granulosus protocercaria group was significant (P < 0.05). Compared with the specific inhibitor DAPT group, the proliferation significantly increased in the Echinococcus granulosus protocercaria + specific inhibitor DAPT group (P < 0.05). (2) The protein and mRNA expression levels of Notch-1, Jagged-1, and Hes-1 were lower in the specific inhibitor DAPT group than those in the bone marrow mesenchymal stem cell group (P < 0.05). These expression levels in the Echinococcus granulosus protocercaria group were higher than those in the bone marrow mesenchymal stem cell group (P < 0.05); these expression levels were higher in the Echinococcus granulosus protocercaria + specific inhibitor DAPT group than those of the specific inhibitor DAPT group (P < 0.05). (3) In conclusion, Echinococcus granulosus can promote the proliferation of bone marrow mesenchymal stem cells through Notch signaling pathway. This phenomenon may lay a theoretical foundation for the formation mechanism of the outer cyst of Echinococcus granulosus, thus explaining how to obtain immune escape after the host is infected with Echinococcus granulosus. It may even open a new research direction for the prevention and early diagnosis of Echinococcus granulosus.

Key words: stem cell, bone marrow mesenchymal stem cell, echinococcus granulosus, proliferation, Notch, signaling pathway

CLC Number:

Liang Xueqi, Zhou Hui, Wu Jie, Xi Yu, He Jiageng, Qin Le, Chen Xueling, Wu Xiangwei, Sun Fan, Niu Jianhua. Echinococcus granulosus promotes the proliferation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(19): 3004-3010.

Figures/Tables 7

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