miR-210/YWHAG axis with adipocyte proliferation and angiogenesis after autologous fat transplantation

doi:10.12307/2022.010

Abstract

Abstract: BACKGROUND: Autologous adipose tissue had been became an excellent filling material because of its advantages of no immune rejection, convenient material extraction, and convenient operation. The study found that the transplanted adipocyte had been in the environment of ischemia and hypoxia before the reconstruction of the vascular network, and such an environment was easy to induce apoptosis and necrosis of adipocytes. How to accelerate the process of proliferation and angiogenesis and inhibit the apoptosis of fat cells is the focus of research on improving the survival rate of autologous fat transplantation.
OBJECTIVE: To explore the mechanism of the effect of miR-210 on the survival of autologous fat transplantation via YWHAG.
METHODS: After 8 weeks of autologous adipose tissue transplantation in Sprague-Dawley rats, the adipose tissue on the back was stripped. The expression levels of miRNAs in adipose tissue were detected by RT-qPCR. The targeting relationship between miR-210 and YWHAG was validated by dual luciferase reporter gene. Adipocytes were isolated from the fat tissues and divided into miR-210 inhibition group, YWHAG overexpression group, miR-210 and YWHAG overexpression group for cell transfection. The expression levels of vascular endothelial growth factor, Endostatin and YWHAG were detected by western blotting. The effects of the miR-210/YWHAG axis on proliferation, apoptosis and angiogenesis of adipocytes were detected by CCK-8, Annexin V-FITC/PI and angiogenesis assay, respectively.
RESULTS AND CONCLUSION: (1) miR-210 was highly expressed in adipose tissue (P < 0.001). (2) The results of dual luciferase reporter gene indicated that YWHAG was targeting protein of miR-210 (P < 0.05). (3) Knockdown of miR-210 significantly inhibited proliferation, angiogenesis and induced apoptosis of adipocytes (P < 0.05). (4) Overexpression of miR-210 and YWHAG at the same time had no significant effect on adipocyte proliferation, apoptosis and angiogenesis. (5) In summary, miR-210 promoted autologous fat graft survival by downregulating the expression level of YWHAG to promote proliferation, angiogenesis and inhibiting apoptosis of adipocytes.

Key words: autologous fat transplantation, mir-210, YWHAG, cell proliferation, angiogenesis, apoptosis

CLC Number:

Yao Yanyi, Chen Xiaoling, Zhang Min, Wang Hong, Hao Liying, Shi Chao, Zhao Bingfeng, Jin Yaqiao, Dong Wei. miR-210/YWHAG axis with adipocyte proliferation and angiogenesis after autologous fat transplantation[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(1): 59-63.

Figures/Tables 5

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