中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3667-3670.doi: 10.3969/j.issn.1673-8225.2012.20.013

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

A型肉毒毒素可抑制人增生性瘢痕成纤维细胞增殖和胶原蛋白的合成★

李卫华1，李德水1，高玉伟2，邢玉玺1   

  1. 1武警医学院附属医院烧伤整形科，天津市   300162；2邢台博爱医院整形科，河北省邢台市054001
  • 收稿日期:2011-09-06 修回日期:2011-11-07 出版日期:2012-05-13 发布日期:2012-05-13
  • 作者简介:李卫华★，男，1971年生，河北省邢台市人，汉族，1999年解放军第四军医大学毕业，硕士，副主任医师，主要从事瘢痕防治与细胞培养的研究。lwh_689@yahoo.com.cn

Effect of botulinum toxin type A on the proliferation and collagen synthesis of human hypertrophic scar fibroblasts 

Li Wei-hua1, Li De-shui1, Gao Yu-wei2, Xing Yu-xi1   

  1. 1Department of Burn and Plastic Surgery, the Affiliated Hospital of Medical College of Chinese People’s Armed Police  Force, Tianjin  300162, China; 2Department of Plastic Surgery, Xingtai Boai Hospital, Xingtai  054001, Hebei Province, China
  • Received:2011-09-06 Revised:2011-11-07 Online:2012-05-13 Published:2012-05-13
  • About author:Li Wei-hua★, Master, Associate chief physician, Department of Burn and Plastic Surgery, the Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China lwh_689@yahoo.com.cn

PDF

639

摘要：

背景：A型肉毒毒素伤口周围局部注射可以减少瘢痕的形成，而且对瘢痕的增生挛缩有抑制作用，可以促进瘢痕的萎缩变平。
目的：观察A型肉毒毒素对人增生性瘢痕成纤维细胞的增殖和胶原蛋白合成的影响。
方法：体外分离、培养人增生性瘢痕成纤维细胞，取对数生长期的细胞接种培养，使用稀释浓度为0.1 U/L A型肉毒素DMEM细胞培养基对细胞的生长过程进行干扰，对照组以含胎牛血清的DMEM培养基培养。
结果与结论：细胞接种第1~15天，对照组可见梭形的增生性瘢痕成纤维细胞分裂增殖，局部融合成细胞单层，细胞生长旺盛，细胞排列呈现高度一致性。A型肉毒毒素组细胞增殖速度慢，细胞数量少，细胞排列方向散乱，A型肉毒毒素组细胞数为对照组细胞数的79.3%，A型肉毒毒素实验组胶原蛋白合成量为正常对照组的48.4%，差异有显著性意义(P < 0.05)。提示A型肉毒毒素可以抑制人增生性瘢痕成纤维细胞的增殖以及胶原蛋白的合成。
 

关键词: 增生性瘢痕, 成纤维细胞, A型肉毒毒素, 细胞培养, 胶原蛋白

Abstract:

BACKGROUND: Local injection of botulinum toxin type A (BTA) around the wound can reduce the scar formation. The scar hyperplasia and contracture can also be inhibited; thereby the scar can become atrophic and flat.
OBJECTIVE: To investigate the effect of BTA on the proliferation and collagen synthesis at human hypertrophic scar fibroblasts.
METHODS: Human hypertrophic scar fibroblasts were isolated and cultured in vitro. The cells at logarithmic growth phase were cultured. Dulbecco's modified Eagle medium (DEME) containing BTA (diluted concentration of 0.1 U/L) was used to intervene the cell growth. Control group used DEME containing fetal bovine serum.
RESULTS AND CONCLUSION: At 1 to 15 days after cells seeding, the hypertrophic scar fibroblasts showed the spindle-like shape with uniform composition and strong proliferation, the fibroblasts proliferated into monolayers, and the hypertrophic cells showed a high degree of consistency in the control group. The cells in the BTA group grew slowly and the arrangement of cells was scattered. The amount of cells in the BTA group was 79.3% of that in the control group. The collagen synthetic rate of BTA group was 48.4% of that in the control group. It is indicated that BTA can inhibit the proliferation and collagen synthesis of human hypertrophic scar fibroblasts.

中图分类号: