中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2823-2826.doi: 10.3969/j.issn.1673-8225.2012.15.036

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

尾吊影响大鼠比目鱼肌Dystrophin表达分布以及血清乳酸脱氢酶活性***★◆

马乐萍1，戴钟铨2，吴  峰2，王红晖2，曹宏卿2，万玉民2，刘鸿宇1，李莹辉2   

  1. 1中北大学生理学实验室、运动医学研究所，山西省太原市  030051；2中国航天员科研训练中心，航天医学基础与应用国家重点实验室，北京市  100094
  • 收稿日期:2012-02-09 修回日期:2012-02-27 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 戴钟铨，博士，副研究员，中国航天员科研训练中心，航天医学基础与应用国家重点实验室，北京市 100094 daizhq77@163.com 并列通讯作者：刘鸿宇，博士，教授，中北大学生理学实验室、运动医学研究所，山西省太原市 030051 lhyxian@nuc.edu.cn
  • 作者简介:马乐萍★，女，1984年生，山西省文水县人，汉族，中北大学在读硕士，主要从事运动生理学的研究。 leping2005@126.com
  • 基金资助:

    国家重点基础研究发展计划项目(973项目：2011CB707704)，国家自然科学基金(30870589)，航天医学基础与应用国家重点实验室项目(SMFA09A08)。

Hindlimb suspension affects the expression and distribution of Dystrophin in rat soleus and serum lactate dehydrogenase activity  

Ma Le-ping1, Dai Zhong-quan2, Wu Feng2, Wang Hong-hui2, Cao Hong-qing2, Wan Yu-min2, Liu Hong-yu1, Li Ying-hui2   

  1. 1Laboratory of Physiology and Institute of Sport and Exercise Medicine, North University of China, Taiyuan  030051, Shanxi Province, China; 2China Astronaut Research & Training Center, State Key Laboratory of Space Medicine Fundamentals and Application, Beijing  100094, China
  • Received:2012-02-09 Revised:2012-02-27 Online:2012-04-08 Published:2012-04-08
  • Contact: Corresponding author: Dai Zhong-quan, Doctor, Associate researcher, China Astronaut Research & Training Center, State Key Laboratory of Space Medicine Fundamentals and Application, Beijing 100094, China daizhq77@163.com Corresponding author: Liu Hong-yu, Doctor, Professor, Laboratory of Physiology and Institute of Sport and Exercise Medicine, North University of China, Taiyuan 030051, Shanxi Province, China lhyxian@nuc.edu.cn
  • About author:Ma Le-ping★, Studying for master’s degree, Laboratory of Physiology and Institute of Sport and Exercise Medicine, North University of China, Taiyuan 030051, Shanxi Province, China leping2005@126.com
  • Supported by:

    the Major State Basic Research Development Program of China (973 Program), No. 2011CB707704*; the National Natural Science Foundation of China, No. 30870589*; the Project of the State Key Laboratory of Space Medicine Fundamentals and Application, No. SMFA09A08*

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摘要：

背景：抗肌萎缩蛋白Dystrophin在肌肉运动性损伤时易发生改变。
目的：观察模拟失重效应对大鼠比目鱼肌抗肌萎缩蛋白Dystrophin表达、分布及血清乳酸脱氢酶活性的影响。
方法：采用大鼠后肢尾吊模拟失重效应模型，分别在尾吊1，4，7，10，14 d分离SD大鼠比目鱼肌并提取血清进行检测。
结果与结论：随着尾吊时间的延长，大鼠肌纤维横截面积减小；肌膜上Dystrophin呈现弥散性分布的趋势，甚至出现Dystrophin断裂现象；Dystrophin mRNA表达下降。同时尾吊7 d，大鼠血清乳酸脱氢酶活性升高。提示失重引起的肌萎缩，伴随着Dystrophin mRNA的表达下降和蛋白分布弥散性变化，而乳酸脱氢酶活性变化提示失重性肌萎缩可能与肌损伤的发生有关。
关键词：肌萎缩；失重；比目鱼肌；Dystrophin；乳酸脱氢酶
doi:10.3969/j.issn.1673-8225.2012.15.036

关键词: 肌萎缩, 失重, 比目鱼肌, Dystrophin, 乳酸脱氢酶

Abstract:

BACKGROUND: Dystrophin is the most prone to change in sports injuries. 
OBJECTIVE: To investigate the effects of simulated weightlessness on the Dystrophin expression and distribution in the soleus muscle, as well as the activity changes of lactate dehydrogenase in serum.
METHODS: A rat hindlimb suspension model was used to simulate weightlessness effect. After 1, 4, 7, 10 and 14 days of hindlimb suspension, the soleus and serum were isolated from SD rats for parameter examination.
RESULTS AND CONCLUSION: With the prolonged hindlimb suspension, muscle fiber cross-sectional area decreased and immunofluorescence results showed that Dystrophin expression exhibited a diffuse distribution trend, even appeared with disrupted distribution on the sarcolemma. Quantitative PCR results showed that the hindlimb suspension resulted in reduced expression of Dystrophin mRNA. Lactate dehydrogenase activity during 7-day hindlimb suspension appeared with a significant increase. Muscle atrophy induced by hindlimb suspension is accompanied with diffusion of Dystrophin distribution and decreased expression of Dystrophin mRNA. Activity changes of lactate dehydrogenase indicate that muscular atrophy may be associated with muscle injury.

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