中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (41): 7658-7663.doi: 10.3969/j.issn.2095-4344.2012.41.012

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

脂肪干细胞与三维支架体内外培养构建的组织工程化气管

郭 翔1，管 欣1，赵 珩2   

  1. 1上海交通大学医学院附属第九人民医院胸外科，上海市 200011
    2上海市胸科医院胸外科，上海市 200030
  • 收稿日期:2012-02-08 修回日期:2012-04-22 出版日期:2012-10-07 发布日期:2013-04-09
  • 通讯作者: 赵珩，硕士，主任医师，上海市胸科医院胸外科，上海市 200030 h_zhao28@163.com
  • 作者简介:郭翔★，男，1978年生，山西省长治市人，汉族，2008年上海交通大学医学院毕业，硕士，主治医师，主要从事组织工程化气管的研究。 guoxiangsjtu@163.com

Construction of tissue engineering trachea by in vitro and in vivo culture of adipose-derived stem cells in three-dimensional scaffolds

Guo Xiang1, Guan Xin1, Zhao Heng2   

  1. 1Department of Thoracic Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China
    2Department of Thoracic Surgery, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China
  • Received:2012-02-08 Revised:2012-04-22 Online:2012-10-07 Published:2013-04-09
  • Contact: Zhao Heng, Master, Chief physician, Department of Thoracic Surgery, Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200030, China h_zhao28@163.com
  • About author:Guo Xiang★, Master, Attending physician, Department of Thoracic Surgery, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China guoxiangsjtu@163.com

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498

被引次数

1

摘要：

背景：气管替代物包括自体组织皮瓣、气管同种异体移植、人工材料支架和无活力组织移植等，但均因为相关严重并发症和获取困难等因素使临床应用遇到很大困难。
目的：利用脂肪干细胞与聚乳酸-乙醇酸共聚物(poly-D,L-lactio-co-glycolic acid,PLGA)、聚三亚甲基碳酸酯(poly(trimethylenecarbonate)，PTMC)共聚物支架构建组织工程化气管支架模型。
方法：组织块法原代分离培养SD大鼠脂肪干细胞，脂肪干细胞行流式细胞术及多向分化能力鉴定，分别种植于PLGA-PTMC支架，经体内体外培养后行免疫组织化学及扫描电镜观察。
结果与结论：大鼠脂肪干细胞接种于支架后，呈球形，伸展出伪足，均匀贴附PLGA-PTMC支架，细胞间相互融合成团；经体内培养后，新生毛细血管丰富。PLGA-PTMC支架具有良好的生物相容性，无细胞毒性，其多孔的三维立体状结构适合脂肪干细胞黏附生长。经体内、体外培养得到组织工程化气管模型，新生血管丰富，可以作为有效的气管替代物。

关键词: 组织工程气管, 脂肪干细胞, 支架, 生物相容性, 组织块培养法, 干细胞

Abstract:

BACKGROUND: Trachea substitutes include autologous tissue flap, allogeneic tracheal transplant, artificial scaffolds and non-dynamic tissue transplant. However, they are hardly used in the clinic because of severe complications and difficult harvest.
OBJECTIVE: To establish the tissue-engineered tracheal scaffold model by adipose-derived stem cells, poly-D,L-lactio-co-glycolic acid and poly(trimethylenecarbonate).
METHODS: Adipose-derived stem cells of Sprague Dawley rats were isolated and cultured by tissue block method. Cell surface markers were evaluated by flow cytometry and multilineage differentiation, and the cells were implanted on the poly-D,L-lactio-co-glycolic-poly(trimethylenecarbonate) scaffold. After in vitro and in vivo culture, the adipose-derived stem cells were observed by immunohistochemistry and scanning electron microscope.
RESULT AND CONCLUSION: Adipose-derived stem cells were spherical and well attached to the scaffolds with lamellipodia and conglobate connection after inoculated on the poly-D,L-lactio-co-glycolic-poly(trimethylenecarbonate) scaffold. After in vitro culture, the newborn capillary could be seen. The poly-D,L-lactio-co-glycolic- poly(trimethylenecarbonate) scaffold scaffolds with porous structure were highly biocompatible and non-cytotoxic as determined by adipose-derived stem cells adhesion and proliferation. Neotrachea fabricated with combined in vitro and in vivo culture system using poly-D,L-lactio-co-glycolic-poly(trimethylenecarbonate) scaffold and adipose-derived stem cells can meet the requirement of tracheal replacement, this model serve as a new way to engineer a vascularized trachea.

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