中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (2): 443-450.doi: 10.12307/2025.494

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

梯度玻璃分级超透氧化锆的体外生物相容性

张其娅1，童伊翔1，杨世姣1，张宇梦1，邓  凌1，吴  玮2，解  瑶3，廖  健1，毛  岭1   

  1. 1贵州医科大学口腔医学院/附属口腔医院，贵州省贵阳市   550004；2 贵州省人民医院，贵州省贵阳市   550002；3平坝区人民医院，贵州省安顺市   561199
  • 收稿日期:2024-07-20 接受日期:2024-09-07 出版日期:2026-01-18 发布日期:2025-07-01
  • 通讯作者: 毛岭，副教授，贵州医科大学口腔医学院/附属口腔医院，贵州省贵阳市 550004
  • 作者简介:张其娅，女，1999年生，贵州省安龙县人，汉族，在读专业型硕士，主要从事口腔种植修复的基础与临床研究。
  • 基金资助:
    贵州省卫生健康委科学技术基金项目(gzwjkj2021-1-342)，项目负责人：毛岭；贵州医科大学博士启动资金(校博合J字[2020]11号)，项目负责人：毛岭

In vitro biocompatibility of graded glass infiltrated ultra-translucent zirconia

Zhang Qiya1, Tong Yixiang1, Yang Shijiao1, Zhang Yumeng1, Deng Ling1, Wu Wei2, Xie Yao3, Liao Jian1, Mao Ling1   

  1. 1School/Hospital of Stomatology, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2Guizhou Provincial People’s Hospital, Guiyang 550002, Guizhou Province, China; 3Pingba District People's Hospital, Anshun 561199, Guizhou Province, China
  • Received:2024-07-20 Accepted:2024-09-07 Online:2026-01-18 Published:2025-07-01
  • Contact: Mao Ling, Associate professor, School/Hospital of Stomatology, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • About author:Zhang Qiya, Master candidate, School/Hospital of Stomatology, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Supported by:
    Science and Technology Fund Project of Guizhou Provincial Health Commission, No. gzwjkj2021-1-342 (to ML); Doctoral Start-up Fund of Guizhou Medical University, No. XBHJZ [2020]11 (to ML)

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摘要：

文题释义：
梯度玻璃分级超透氧化锆：使用内部研发的玻璃材料，运用玻璃渗透技术对5Y-PSZ超透氧化锆进行性能改进，在保持5Y-PSZ良好半透明性的优势同时提高超透明氧化锆5Y-PSZ抗弯强度后的材料。
生物相容性：指生物材料在宿主特定的环境和部位与宿主直接或间接接触时所产生的相互反应的能力。生物相容性包括血液相容性和组织相容性。

摘要
背景：前期研究通过2次烧结法将玻璃材料渗透入5Y-PSZ超透氧化锆中，制备了能保持高透明性且抗弯强度较高的5Y-PSZ-YGI梯度玻璃分级超透氧化锆材料。
目的：评估5Y-PSZ-YGI梯度玻璃分级超透氧化锆材料的体外生物相容性。
方法：①通过2次烧结将玻璃材料渗透入5Y-PSZ超透氧化锆中，制备5Y-PSZ-YGI梯度玻璃分级氧化锆。将5Y-PSZ-YGI梯度玻璃分级超透氧化锆(或5Y-PSZ超透氧化锆、3Y-TZP透明氧化锆)置入含体积分数10%胎牛血清DMEM培养基中12，24，72 h，培养基与试样表面积比为3 mL/cm2，得到12，24，72 h材料浸提液。②将小鼠成纤维细胞L929培养24 h后弃原培养基，分7组培养：对照组更换为含体积分数10%胎牛血清DMEM培养基，其余6组分别更换为3Y-TZP透明氧化锆24 h浸提液、5Y-PSZ超透氧化锆24 h浸提液、5Y-PSZ-YGI梯度玻璃分级超透氧化锆24 h浸提液、3Y-TZP透明氧化锆72 h浸提液、5Y-PSZ超透氧化锆72 h浸提液、5Y-PSZ-YGI梯度玻璃分级超透氧化锆72 h浸提液。培养1，3，5 d后，显微镜下观察细胞生长，通过CCK-8检测得到细胞增殖率，判定细胞毒性。③将人抗凝血分别与5Y-PSZ-YGI梯度玻璃分级超透氧化锆、5Y-PSZ超透氧化锆、3Y-TZP透明氧化锆混合，0.5 h后检测溶血率。将人抗凝血分别与3Y-TZP透明氧化锆12 h 浸提液、5Y-PSZ超透氧化锆12 h浸提液、5Y-PSZ-YGI梯度玻璃分级超透氧化锆12 h浸提液混合，0.5 h后检测溶血率。
结果与结论：①显微镜下可见，随着培养时间的延长，各组细胞数量增加，并且各实验组细胞形态与对照组基本一致。3Y-TZP透明氧化锆24 h浸提液组培养第1天的细胞毒性分级 0级，其余实验组在各个时间段的细胞毒性分级均为1级。②无论是材料还是材料浸提液均未引起明显的溶血反应，溶血率均低于5%。③结果表明，5Y-PSZ-YGI梯度玻璃分级超透氧化锆对小鼠成纤维细胞L929生长增殖无明显影响，并且不会与人血发生溶血反应，具有良好的体外生物相容性。
https://orcid.org/0009-0000-3327-919X (张其娅)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料；口腔生物材料；纳米材料；缓释材料；材料相容性；组织工程

关键词: 梯度玻璃分级超透氧化锆, 生物相容性, 超透氧化锆, 透明氧化锆, 细胞毒性, 溶血率, 生物材料, 工程化口腔材料

Abstract: BACKGROUND: In previous studies, glass materials were infiltrated into 5Y-PSZ ultra-translucent zirconia by a double sintering method to prepare 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia materials that can maintain high transparency and high flexural strength.
OBJECTIVE: To evaluate the in vitro biocompatibility of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia materials.
METHODS: (1) Glass materials were infiltrated into 5Y-PSZ ultra-translucent zirconia by double sintering to prepare 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia. 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia (or 5Y-PSZ ultra-translucent zirconia, 3Y-TZP transparent zirconia) was placed in DMEM culture medium containing 10% fetal bovine serum for 12, 24 and 72 hours, and the surface area ratio of culture medium to sample was 3 mL/cm2, and the 12-, 24- and 72-hour material extracts were obtained. (2) After culturing mouse fibroblast L929 for 24 hours, the original culture medium was discarded and divided into 7 groups for culture: the control group was replaced with DMEM culture medium containing 10% fetal bovine serum by volume, and the other 6 groups were replaced with 24-hour extract of 3Y-TZP transparent zirconia, 24-hour extract of 5Y-PSZ ultra-translucent zirconia, 24-hour extract of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia, 72-hour extract of 3Y-TZP transparent zirconia, 72-hour extract of 5Y-PSZ ultra-translucent zirconia, and 72-hour extract of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia. After 1, 3, and 5 days of culture, cell growth was observed under a microscope, and the cell proliferation rate was obtained by CCK-8 assay to determine cytotoxicity. (3) Human anticoagulated blood was mixed with 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia, 5Y-PSZ ultra-translucent zirconia, and 3Y-TZP transparent zirconia, and the hemolysis rate was detected after 0.5 hours. Human anticoagulated blood was mixed with 12-hour extract of 3Y-TZP transparent zirconia, 12-hour extract of 5Y-PSZ ultra-translucent zirconia, and 12-hour extract of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia, and the hemolysis rate was detected after 0.5 hours.
RESULTS AND CONCLUSION: (1) Under the microscope, it could be seen that the number of cells in each group increased with the extension of culture time, and the cell morphology of each experimental group was basically the same as that of the control group. The cytotoxicity grade of the 24-hour extract of 3Y-TZP transparent zirconia group on the first day of culture was grade 0, and the cytotoxicity grade of the other experimental groups at each time period was grade 1. (2) Neither the material nor the material extract caused obvious hemolytic reaction, and the hemolytic rate was less than 5%. (3) The results showed that 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia had no significant effect on the growth and proliferation of mouse fibroblasts L929, and did not cause hemolytic reaction with human blood, and had good in vitro biocompatibility.


Key words: graded glass infiltrated ultra-translucent zirconia, biocompatibility, ultra-translucent zirconia, transparent zirconia, cytotoxicity, hemolysis rate, biomaterial, engineered oral material

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