中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5936-5940.doi: 10.3969/j.issn.1673-8225.2011.32.011

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

脂肪与骨髓来源间充质干细胞生物学特性的比较

朱希山，施  薇，台卫平，安广宇   

  1. 首都医科大学附属北京世纪坛医院(北京大学第九临床医学院)肿瘤内科，北京市
    100038
  • 收稿日期:2011-03-07 修回日期:2011-06-13 出版日期:2011-08-06 发布日期:2011-08-06
  • 作者简介:朱希山☆，男，山东省潍坊市人，汉族，2009年北京协和医学院(清华大学医学部)毕业，博士，主要从事肿瘤干细胞的基础研究和临床应用。 zhuxishan@tsinghua.org.cn

Comparison of the biological characteristics of bone marrow and adipose tissue derived mesenchymal stem cells

Zhu Xi-shan, Shi Wei, Tai Wei-ping, An Guang-yu   

  1. Department of Tumor, Beijing Shijitan Hospital, Capital Medical University (The 9th Hospital of Peking University), Beijing  100038, China
  • Received:2011-03-07 Revised:2011-06-13 Online:2011-08-06 Published:2011-08-06
  • About author:Zhu Xi-shan☆, Doctor, Department of Tumor, Beijing Shijitan Hospital, Capital Medical University (The 9th Hospital of Peking University), Beijing 100038, China zhuxishan@tsinghua.org.cn

PDF

432

被引次数

10

摘要：

背景：近几年来脂肪来源的间充质干细胞因其取材容易也被广泛研究。
目的：比较脂肪来源和骨髓来源间充质干细胞的生物学特性。
方法：分离及体外培养人骨髓源间充质干细胞和脂肪源间充质干细胞，比较它们的表型、细胞倍增时间及分泌因子水平等。
结果与结论：脂肪来源和骨髓来源的间充质干细胞在细胞表型上类似，只有CD106的表达有差异。脂肪来源间充质干细胞增殖速率比骨髓来源的间充质干细胞快。在相同体积的脂肪组织中能够得到的干细胞前体细胞的数量是骨髓的10倍以上。提示脂肪来源和骨髓来源的间充质干细胞具有相同功能，但脂肪组织是一个更有应用前景的干细胞来源。

关键词: 脂肪间充质干细胞, 骨髓间充质干细胞, 生物学特性, 表型, 周期

Abstract:

BACKGROUND: Recently, adipose tissue derived mesenchymal stem cells due to easy to be harvested have been widely studied.
OBJECTIVE: To compare the biological characteristics of adipose tissue derived mesenchymal stem cells and bone marrow mesenchymal stem cells.
METHODS: Adipose tissue derived mesenchymal stem cells and bone marrow mesenchymal stem cells were isolated and cultured in vitro to compare their phenotype, cell doubling time and the level of secreted factors.
RESULTS AND CONCLUSION: Adipose tissue derived mesenchymal stem cells have similar phenotype and differentiation ability as bone marrow mesenchymal stem cells except CD106 expression. The frequency of mesenchymal stem cells in bone marrow and adipose tissue was also calculated and found that mesenchymal stem cells frequency in adipose tissue was 10 times higher than bone marrow. This result can be an instruction for mesenchymal stem cells clinical use.

中图分类号: