中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (3): 447-451.doi: 10.3969/j.issn.1673-8225.2010.03.016

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

聚乙二醇-聚乙烯亚胺/四氧化三铁纳米磁流体的毒性及其生物相容性

邹  芬1，潘一峰2，张  红2，李国政2，唐静波2，彭  健2   

  1. 1中山市华南现代中医药城发展有限公司，广东省中山市  528451；  2 中南大学卫生部纳米生物技术重点实验室，湖南省长沙市  410008
  • 出版日期:2010-01-15 发布日期:2010-01-15
  • 通讯作者: 彭 健，副教授，博士后，硕士生导师，中南大学卫生部纳米生物技术重点实验室，湖南省长沙市 410008
  • 作者简介:邹 芬★，女，1981年生，湖南省怀化市人，2008年中南大学毕业，硕士，主要从事纳米药物载体方面的研究。 miffysweet@126.com
  • 基金资助:

    国家自然科学基金项目(30672047) ，课题名称：治疗肝癌的自杀基因靶向磁纳米载体研究。湖南省研究生创新基金项目(2340-74335000013)，课题名称：磁性纳米粒磁感应靶向性肿瘤热化疗仪的研究。

Toxicity and biocompatibility of polyethylene glycol-polyethyleneimine/ferroso-ferric oxide nano-magnetic fluid

Zou Fen1, Pan Yi-feng2, Zhang Hong2, Li Guo-zheng2, Tang Jing-bo2, Peng Jian2   

  1. 1 Modern Chinese Medicine Park in South China Co., Ltd, Zhongshan  528451, Guangdong Province, China; 2 National Key Laboratory of Nanobiological Technology, South China University, Changsha  410008, Hunan Province, China
  • Online:2010-01-15 Published:2010-01-15
  • Contact: Peng Jian, Doctor, Associate professor, Master’s supervisor, National Key Laboratory of Nanobiological Technology, South China University, Changsha 410008, Hunan Province, China
  • About author:Zou Fen★, Master, Modern Chinese Medicine Park in South China Co., Ltd, Zhongshan 528451, Guangdong Province, China miffysweet@126.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30672047*; Postgraduate Innovation Fund of Hunan Province, No. 2340-74335000013*

PDF

865

被引次数

38

摘要：

背景：为实现纳米基因药物在治疗肿瘤中实现高靶向性，课题采用聚乙二醇和聚乙烯亚胺修饰的磁性四氧化三铁纳米粒(PEG-PEI/Fe3O4)作为基因药物载体，在提高载体载药能力的同时，使载体具有超顺磁性，增加药物靶向性能。
目的：评价PEG-PEI/Fe3O4纳米磁流体体外和体内毒性。
方法：对制备的PEG-PEI/Fe3O4纳米磁流体进行表征达到纳米材料水平后，过滤稀释5~20倍培养7702细胞和HpG2细胞，进行体外MTT毒性试验；通过体内溶血试验和微核试验测定生物相容性和体内毒性。
结果与结论：MTT结果显示，PEG-PEI/Fe3O4纳米磁流体对7702细胞毒性0~1级，对正常肝细胞无害；PEG-PEI/Fe3O4纳米磁流体对HpG2细胞有轻微的旁观者抑制效应；PEG-PEI/Fe3O4纳米磁流体溶血率为0.372%，远小于5%；微核试验结果表明PEG-PEI/Fe3O4纳米磁流体无致畸、致突变作用。

关键词: 磁流体, 细胞毒性试验, 生物相容性, 溶血试验, 微核试验

Abstract:

BACKGROUND: Polyethylene glycol-polyethyleneimine/ferroso-ferric oxide (PEG-PEI/Fe3O4) was selected as drug carriers in tumor treatment, which can increase drug loading capacity and targeting capacity.
OBJECTIVE: To test the toxicity of PEG-PEI/Fe3O4 nano-magnetic fluid in vitro and in vivo.
METHODS: When the prepared PEG-PEI/Fe3O4 nano-magnetic fluid reached nano level, 7702 and HpG2 cell lines were filtrated and diluted in 5-20 multiple, and detected by in vitro MTT toxicity test assay; in vivo hemolysis test and micronucleus test was used to test the toxicity and biocompatibility.
RESULTS AND CONCLUSION: MTT assay results indicated that the toxicity grade of PEG-PEI/Fe3O4 nano-magnetic fluid to 7702 cell line was 0-1, which was harmless to natural hepatic cells; however, PEG-PEI/Fe3O4 nano-magnetic fluid had slight bystander restraining effect to HpG2 cell line. Maximum hemolysis rate of the material was 0.372%, which was far less than 5%. The micronucleus test result indicated that PEG-PEI/Fe3O4 nano-magnetic fluid had no teratogenicity or mutagenicity.

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