中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (48): 9039-9043.doi: 10.3969/j.issn.2095-4344.2012.48.021

• 脊柱损伤基础实验 basic experiments of spinal injury • 上一篇    下一篇

神经肽Y/降钙素基因相关肽在腰椎间盘中的分布与共表达

王永刚，周海宇，王栓科，王东敏，时培晟，汪 静，王翠芳，马靖琳   

  1. 兰州大学第二医院骨科，甘肃省骨关节疾病重点实验室，甘肃省兰州市 730030
  • 收稿日期:2012-02-22 修回日期:2012-05-24 出版日期:2012-11-25 发布日期:2012-11-25
  • 通讯作者: 周海宇，副教授，副主任医师，兰州大学第二医院骨科，甘肃省骨关节疾病重点实验室，甘肃省兰州市 730030 zhouhy@lzu.edu.cn
  • 作者简介:王永刚，男，1978年生，宁夏回族自治区固原市人，汉族，医师，主要从事脊柱骨科的研究。 13462717@qq.com

Distribution and co-expression of neuropeptide Y/calcitonin gene-related peptide in the lumbar intervertebral disc

Wang Yong-gang, Zhou Hai-yu, Wang Shuan-ke, Wang Dong-min, Shi Pei-sheng, Wang Jing, Wang Cui-fang, Ma Jing-lin   

  1. Department of Orthopedics, Second Hospital of Lanzhou University, Key Laboratory of Bone and Joint Disease in Gansu Province, Lanzhou 730030, Gansu Province, China
  • Received:2012-02-22 Revised:2012-05-24 Online:2012-11-25 Published:2012-11-25
  • Contact: Zhou Hai-yu, Associate professor, Associate chief physician, Department of Orthopedics, Second Hospital of Lanzhou University, Key Laboratory of Bone and Joint Disease in Gansu Province, Lanzhou 730030, Gansu Province, China zhouhy@lzu.edu.cn
  • About author:Wang Yong-gang, Physician, Department of Orthopedics, Second Hospital of Lanzhou University, Key Laboratory of Bone and Joint Disease in Gansu Province, Lanzhou 730030, Gansu Province, China 13462717@qq.com

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852

被引次数

9

摘要：

背景：研究发现，神经肽Y与降钙素基因相关肽在交感神经节中有共存现象。
目的：观察神经肽Y/降钙素基因相关肽在正常腰椎间盘的共分布及在突出腰椎间盘髓核组织中的共表达。
方法：从10例尸体中收集完整的腰椎间盘，在另10例尸体中收集腰椎间盘髓核组织作为对照组。收集30例有症状的腰椎间盘突出症患者，其中L4/5或L5/S1需行腰椎间盘髓核摘除，取出髓核组织作为实验组。
结果与结论：①正常椎间盘组织中神经肽Y/降钙素基因相关肽荧光双标染色阳性的神经纤维较多的分布于椎间盘纤维环外1/3，但在椎间盘内2/3及髓核中未见或少量分布。②髓核的共表达，神经肽Y/降钙素基因相关肽荧光双标染色神经纤维的阳性率实验组均高于对照组(P < 0.05)。提示在正常腰椎间盘组织中神经肽Y/降钙素基因相关肽分布于纤维环外1/3部分，在纤维环内2/3部分及髓核组织未分布，但在突出椎间盘髓核组织中有大量的神经肽Y/降钙素基因相关肽共表达。

关键词: 神经肽Y, 降钙素基因相关肽, 慢性下腰痛, 腰椎间盘, 髓核, 荧光双标染色, 组织工程

Abstract:

BACKGROUND: Studies have found the co-existence of neuropeptide Y/calcitonin gene-related peptide in the sympathetic ganglia.
OBJECTIVE: To study the distribution of neuropeptide Y/calcitonin gene-related peptide in the normal lumbar disc and the co-expression in herniated lumbar nucleus pulposus tissue.
METHODS: Complete lumbar discs were collected from 10 cases of cadaver, and herniated lumbar nucleus pulposus tissues were collected from other 10 cases of cadaver as control. Thirty patients with the symptoms lumbar disc herniation were collected, lumbar nucleus pulposus of L4/5 and L5/S1 were removed and the nucleus pulposus tissues were obtained as control group.
RESULTS AND CONCLUSION: ①Positive neuropeptide Y/calcitonin gene-related peptide immunofluorescence double staining of nerve fibers were distributed in the 1/3 outside annulus fibrosus and few in 2/3 inside annulus fibrosus and nucleus pulposus in the normal lumbar intervertebral disc tissue. ②Co-expression of nucleus pulposus and the positive rate of neuropeptide Y/calcitonin gene-related peptide immunofluorescence double staining of nerve fibers in the experimental group were higher than those in the control group (P < 0.05). This study demonstrates that neuropeptide Y/calcitonin gene-related peptide distributes on the 1/3 outside annulus fibrosus in normal lumbar intervertebral disc, but do not distribute on the 2/3 inside of annulus fibrosus and nucleus pulposus. But a large number of neuropeptide Y/calcitonin gene-related peptide co-expressed in the herniated nucleus pulposus.

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