中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (51): 9587-9590.doi: 10.3969/j.issn.1673-8225.2010.51.019

• 药物控释材料 drug delivery materials • 上一篇    下一篇

复方中药聚乳酸微球兔膝骨性关节炎关节腔注入

赵洪普1，徐秋玉2，杨  帆3   

  1. 1广州医学院第三附属医院骨科，广东省广州市 510150；2广州中医药大学第一附属医院门诊部，广东省广州市 510405；3广东药学院药科学院，广东省广州市  510224
  • 出版日期:2010-12-17 发布日期:2010-12-17
  • 作者简介:赵洪普☆，男，1963年生，河北省保定市人，汉族，2000年日本九州大学研究生院毕业，博士，副教授，主要从事关节外科研究。 hongpuzhao@hotmail.com
  • 基金资助:

    广东省中医药局项目(103006)；广东省卫生科技基金项目(A2008289)；广东省科技计划项目(粤科函社字[2010]1096号)。

Intra-articular injection of polylactic acid microspheres containing compound Chinese herbal medicine into rabbits with knee osteoarthritis

Zhao Hong-pu1, Xu Qiu-yu2, Yang Fan3   

  1. 1 Department of Orthopaedic Surgery, Third Affiliated Hospital of Guangzhou Medical Collage, Guangzhou   510150, Guangdong Province, China; 2 Department of Outpatient, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou   510405, Guangdong Province, China; 3 Department of Pharmacy, Guangdong College of Pharmacy, Guangzhou   510224, Guangdong Province, China
  • Online:2010-12-17 Published:2010-12-17
  • About author:Zhao Hong-pu☆, Doctor, Associate professor, Department of Orthopaedic Surgery, Third Affiliated Hospital of Guangzhou Medical Collage, Guangzhou 510150, Guangdong Province, China hongpuzhao@hotmail.com
  • Supported by:

    Traditional Chinese Medicine Administration of Guangdong Province, No. 103006*; Health Science and Technology Foundation of Guangdong Province, No. A2008289*; Science and Technology Project of Guangdong Province, No. 20101096*

PDF

333

被引次数

9

摘要：

背景：骨炎1号方由淫羊藿、补骨脂、当归三味中药组成，具有补肝益肾，强壮筋骨，活血化瘀，通络止痛的作用。
目的：观察骨炎1号聚乳酸微球膝关节腔内注射干预骨性关节炎的可行性。
方法：将新西兰大白兔以数字表法随机分成生理盐水组、玻璃酸钠组、骨炎1号聚乳酸微组。所有动物的右膝经手术制成骨性关节炎模型。造模后6周开始关节内干预注射生理盐水、玻璃酸钠、骨炎1号聚乳酸微球，1次/周，连续给药5次。最后一次给药后2周在解剖显微镜下对患膝进行大体解剖学评分，并对关节软骨进行组织形态学观察。
结果与结论：与生理盐水组相比，骨炎1号聚乳酸微球组和玻璃酸钠组均可降低实验动物的关节炎指数(P < 0.05)、减轻关节软骨的退变。骨炎1号聚乳酸微球组对关节的保护作用不及玻璃酸钠组，但两组间差异无显著性意义。说明骨炎1号聚乳酸微球制剂关节腔内注射干预骨性关节炎是可行的，对动物骨性关节炎具有一定的治疗作用。

关键词: 骨性关节炎, 骨炎1号, 聚乳酸微球, 关节腔注射, 生物材料与药物控释

Abstract:

BACKGROUND: Guyan Yihao is composed by epimedium, psoralen and angelica. It has effects on invigorating liver and tonifying kidney, strengthening the muscles and bones, promoting blood circulation by removing blood stasis, removing obstruction in the channels to relieve pain.
OBJECTIVE: To explore the availability of intra-articular injection of Guyan Yihao microspheres on the osteoarthritis of rabbit’s knee.
METHODS: New Zealand white rabbits were divided randomly into normal saline-treated group, sodium hyaluronate-treated group, and Guyan Yihao polylactide microsphere-treated group. All of their right knees were made osteoarthritis model with Hulth method. At 6 weeks after operating, the animals were injected respectively with normal saline, sodium hyaluronate, and Guyan Yihao polylactide microspheres into the right knee once a week for 5 times. Two weeks after the last injection, the animals were sacrificed and the affected knee joints were harvested for gross anatomical and histomorphological evaluation.
RESULTS AND CONCLUSION: In Guyan Yihao polylactide microsphere-treated group and sodium hyaluronate-treated group, the arthritis index was decreased significantly compared with saline-treated group (P < 0.05), and articular cartilage cataplasia was relieved. There were no significant differences between Guyan Yihao polylactide microsphere and sodium hyaluronate, but Guyan Yihao was less effective than sodium hyaluronate. Guyan Yihao polylactide microspheres are available in intra-articular injection for osteoarthritis treatment. Some protective effects of Guyan Yihao on arthritis is showed in this study.

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