中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (2): 298-301.doi: 10.3969/j.issn.1673-8225.2010.02.025

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

前交叉韧带切断兔外侧半月板中白细胞介素1β的表达

李国军1，李康华2，张世清1，王  晓1　   

  1. 1河南大学淮河医院骨科，河南省开封市  475001；  2中南大学湘雅医院骨科，湖南省长沙市  410008
  • 出版日期:2010-01-08 发布日期:2010-01-08
  • 作者简介:李国军☆，男， 1971年生，河南省尉氏县人，汉族，2009年中南大学毕业，博士，主治医师，主要从事骨与关节损伤的研究。 lgjhndx@yahoo.com.cn

Expression of interleukin 1 beta in the lateral meniscus of rabbits with anterior cruciate ligament rupture

Li Guo-jun1, Li Kang-hua2, Zhang Shi-qing1, Wang Xiao1   

  1. 1 Department of Orthopaedics, Huaihe Hospital of Henan University, Kaifeng  475001, Henan Province, China; 2 Department of Orthopaedics, Xiangya Hospital of Central South University, Changsha  410008, Hunan Province, China
  • Online:2010-01-08 Published:2010-01-08
  • About author:Li Guo-jun☆, Doctor, Attending physician, Department of Orthopaedics, Huaihe Hospital of Henan University, Kaifeng 475001, Henan Province, China lgjhndx@yahoo.com.cn

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摘要：

背景：研究证明，膝关节前交叉韧带切断后，白细胞介素1β是关节软骨退变中一个重要的调控因子，然而白细胞介素1β在外侧半月板退变中有何作用，未见文献报道。
目的：观察兔前交叉韧带切断后外侧半月板中白细胞介素1β阳性表达率的变化并探讨其意义。
方法：6月龄雄性新西兰大白兔48只，将兔膝关节配对为实验侧和对照侧造模。实验侧行前交叉韧带切断手术，制作兔前交叉韧带断裂模型；对照侧显露前交叉韧带但不行切断。造模后第1，3，6，8周，分别麻醉下随机处死兔12只。进行大体观察和外侧半月板组织学退变评分，免疫组织化学检测白细胞介素1β阳性表达率。
结果与结论：前交叉韧带切断后，随时间延长，实验侧外侧半月板组织学退变评分逐渐增高，实验侧各时间组织学退变评分显著高于对照组(P < 0.05)。各时间点实验侧白细胞介素1β阳性表达率均较对照组增高(P < 0.05)；第3，6周实验侧白细胞介素1β表达阳性率高于第1，8周(P < 0.05)，第8周高于第1周(P< 0.05)。结果表明，兔前交叉韧带切断后实验侧白细胞介素1β阳性表达率增高，提示白细胞介素1β参与了外侧半月板组织的退变。白细胞介素1β阳性表达率降低并不表示外侧半月板组织退变结束。

关键词: 前交叉韧带, 半月板, 外侧, 白细胞介素1&beta

Abstract:

BACKGROUND: Previous studies demonstrate that interleukin 1β (IL-1β) plays a great role in the degeneration of articular cartilage in knee joints which was caused by the anterior cruciate lagement (ACL) rupture; however, the role of IL-1β in the degeneration of lateral meniscus remains poorly understood.
OBJECTIVE: To investigate the changes and significance of the expression of IL-1β in the lateral meniscus of rabbits with ACL rupture.
METHODS: A total of 48 male, New Zealand, white rabbits, were matched mode pairs and prepared for ACL rupture models. In the experimental side, the ateral meniscus of rabbits was cut down to prepare ACL rupture models, simultaneously, the control sides were exposed without cut down. A total of 12 rabbits were sacrificed under anesthesia at weeks 1, 3, 6 and 8 after model preparation. Degeneration scores of lateral meniscus and expression of IL-1β in lateral meniscus were performed.
RESULTS AND CONCLUSION: With time prolonged, the scores of lateral meniscus degeneration were increased, which was higher in the experimental group than those in the control group at each time points (P < 0.05). The positive rate of IL-1β was greater in experimental group than that in the control group (P < 0.05). In addition, the positive rate of IL-1β in the experimental group was greater at weeks 3 and 6 than weeks 1 and 8 (P < 0.05), which was higher in week 8 than week 1 (P < 0.05). The results demonstrated that the highly expressed IL-1β in the experimental sides hints that IL-1β maybe a promotional factor which causes the degeneration of lateral meniscus. The decreasing of the expression of IL-1β is not a stopping signs of lateral meniscus degeneration.

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