中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (31): 8181-8189.doi: 10.12307/2026.438

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

生长抑素配受体调控肾上腺皮质激素合成的单细胞转录组分析

邓思宇1，2，陈中元3，姜经航4，郭  毅2，郑  杰2，王宏虹2，王逸夫1，莫曾南1，蒋永华1，2   

  1. 1广西医科大学生命科学研究院，广西壮族自治区南宁市   530021；2广西医科大学基因组与个体化医学研究中心，广西基因组与个体化医学研究重点实验室，广西基因组与个体化医学协同创新中心，广西壮族自治区南宁市   530021；3上海健康医学院附属崇明医院，上海市   202150；4荆楚理工学院附属荆门市人民医院，湖北省荆门市   448000
  • 收稿日期:2025-09-17 接受日期:2026-01-24 出版日期:2026-11-08 发布日期:2026-05-25
  • 通讯作者: 蒋永华，博士，教授，广西医科大学生命科学研究院，广西壮族自治区南宁市 530021；广西医科大学基因组与个体化医学研究中心，广西基因组与个体化医学研究重点实验室，广西基因组与个体化医学协同创新中心，广西壮族自治区南宁市 530021
  • 作者简介:邓思宇，女，2001年生，河南省汝南县人，汉族，广西医科大学在读硕士，主要从事分子生物学、甾体激素代谢等方面的研究。 共同第一作者：陈中元，男，1997年生，江苏省建湖县人，汉族，硕士，初级检验技师，主要从事分子生物学方面的研究。
  • 基金资助:
    广西自然科学基金(2021JJA140912)，项目负责人：蒋永华；广西重点研发专项(GuikeAB21196022，GuikeAA22412，GuikeAA22398)，
    项目负责人：莫曾南； 广西创新驱动专项 (AA18118016)，项目负责人：莫曾南；南宁市科学研究与技术开发计划重大项目(20221023)，项目负责人：蒋永华；国家自然科学基金(82460160)，项目负责人：蒋永华；国家自然科学基金(82270806)，项目负责人：莫曾南；湖北省自然科学基金(2024AFB776)，项目负责人：姜经航

Single-cell transcriptomic analysis of somatostatin receptor-mediated regulation of adrenal corticosteroid synthesis

Deng Siyu1, 2, Chen Zhongyuan3, Jiang Jinghang4, Guo Yi2, Zheng Jie2, Wang Honghong2, Wang Yifu1, Mo Zengnan1, Jiang Yonghua1, 2   

  1. 1Institute of Life Sciences, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; 2Center for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; 3Chongming Hospital Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 202150, China; 4Jingmen People's Hospital Affiliated to Jingchu University of Technology, Jingmen 448000, Hubei Province, China
  • Received:2025-09-17 Accepted:2026-01-24 Online:2026-11-08 Published:2026-05-25
  • Contact: Jiang Yonghua, PhD, Professor, Institute of Life Sciences, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Center for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Deng Siyu, MS candidate, Institute of Life Sciences, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China; Center for Genomic and Personalized Medicine, Guangxi Key Laboratory for Genomic and Personalized Medicine, Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Chen Zhongyuan, MS, Junior laboratory technician, Chongming Hospital Affiliated to Shanghai University of Medicine and Health Sciences, Shanghai 202150, China Deng Siyu and Chen Zhongyuan contributed equally to this article.
  • Supported by:
    Guangxi Natural Science Foundation, No. 2021JJA140912 (to JYH); Guangxi Key Research and Development Program, No. GuikeAB21196022, GuikeAA22412, GuikeAA22398 (to MZN); Guangxi Innovation-Driven Development Program, No. AA18118016 (to MZN); Nanning Municipal Science and Technology Development Program Major Project, No. 20221023 (to JYH); National Natural Science Foundation of China, No. 82460160 (to JYH); National Natural Science Foundation of China, No. 82270806 (to MZN); Hubei Provincial Natural Science Foundation, No. 2024AFB776 (to JJH)

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摘要：

文题释义：

生长抑素：全称生长激素抑制激素，是一种关键的环肽激素，通常作为神经和消化道系统中的抑制性信号分子。然而，此文聚焦于生长抑素在肾上腺环境中以往未被充分探索的作用。
肾上腺皮质激素：是肾上腺皮质分泌的类固醇激素，主要包括糖皮质激素(如皮质醇)、盐皮质激素(如醛固酮)和肾上腺雄激素(如脱氢表雄酮)。此次研究通过单细胞转录组分析及体外细胞实验验证生长抑素对肾上腺皮质激素的调控作用，提示其通过生长抑素受体2特异性调控肾上腺皮质激素生成。

摘要
背景：生长抑素是一种常见的神经、消化道环肽激素，然而其在肾上腺的作用机制不清。
目的：基于单细胞转录组和体外实验探索生长抑素对皮质类固醇激素合成的调控作用。
方法：基于单细胞转录组分析胚胎及成人肾上腺中生长抑素配受体表达谱；qRT-PCR和免疫荧光检测分析受体亚型。为了进一步探索生长抑素对皮质激素合成的影响，分别用不同浓度的生长抑素刺激NCI-H295R肾上腺皮质细胞，ELISA检测分析皮质激素脱氢表雄酮、皮质醇、醛固酮及其关键代谢酶(CYP17A1、HSD3B2、CYB5A)的表达变化。
结果与结论：①单细胞转录组分析提示，胚胎及成人肾上腺中，仅少量胚胎期髓质细胞表达生长抑素，成人肾上腺无生长抑素表达；但是胚胎与成人的皮质类固醇细胞均有较多的生长抑素受体2表达；②qRT-PCR提示NCI-H295R细胞高表达生长抑素受体2；③低浓度生长抑素(1 nmol/L)抑制脱氢表雄酮分泌(P < 0.05)，CYB5A表达下调；而10 nmol/L生长抑素则显著促进脱氢表雄酮合成(P < 0.05)，CYB5A上调，有趣的是，不同浓度生长抑素刺激对皮质醇合成均无显著影响；④提示生长抑素可以通过生长抑素受体2双向调控肾上腺皮质细胞的雄激素脱氢表雄酮合成分泌，但对皮质醇和醛固酮合成的影响不明显。

关键词: 单细胞转录组测序, 生长抑素, 生长抑素受体2, 脱氢表雄酮, 皮质醇, 醛固酮

Abstract: BACKGROUND: Somatostatin is a common neuropeptide and gastrointestinal cyclic peptide hormone, but its mechanism of action in the adrenal gland remains unclear.
OBJECTIVE: To investigate the regulatory effect of somatostatin on corticosteroid hormone synthesis using single-cell transcriptomics and in vitro experiments.
METHODS: Single-cell transcriptomic analysis was conducted to examine the expression profile of somatostatin receptors in both embryonic and adult adrenal glands. qRT-PCR and immunofluorescence assays were employed to analyze receptor subtypes. To further explore the effect of somatostatin on corticosteroid synthesis, NCI-H295R adrenocortical cells were stimulated with different concentrations of somatostatin. ELISA was used to detect the expression changes of dehydroepiandrosterone, cortisol, aldosterone, and their key metabolic enzymes (CYP17A1, HSD3B2, and CYB5A).
RESULTS AND CONCLUSION: (1) Single-cell transcriptomic analysis revealed that only a small number of embryonic adrenal medullary cells expressed somatostatin, with no somatostatin expression observed in the adult adrenal gland. However, both embryonic and adult adrenal steroidogenic cells exhibited significant expression of somatostatin receptor 2. (2) qRT-PCR confirmed high expression of somatostatin receptor 2 in NCI-H295R cells. (3) Low concentrations of somatostatin (1 nmol/L) inhibited dehydroepiandrosterone secretion (P < 0.05), and CYB5A expression was downregulated; while 10 nmol/L somatostatin significantly promoted dehydroepiandrosterone synthesis (P < 0.05), and CYB5A was upregulated. Interestingly, different concentrations of somatostatin stimulation had no significant effect on cortisol synthesis. (4) This suggests that somatostatin can bidirectionally regulate the synthesis and secretion of dehydroepiandrosterone in adrenocortical cells through somatostatin receptor 2, but its effect on cortisol and aldosterone synthesis is not significant.

Key words: single-cell RNA sequencing, somatostatin, somatostatin receptor 2, dehydroepiandrosterone, cortisol, aldosterone

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