中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (8): 1155-1160.doi: 10.3969/j.issn.2095-4344.2017.08.002

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

整合素α5β1在膝骨关节炎不同退变程度关节软骨中的差异性表达

刘俊才1,陈友霞2,李  忠1,吴绍军1,左银龙1,李  文1,何  克1,张志伟1,李仁明1   

  1. 西南医科大学附属医院,1骨关与节外科,2 ICU,四川省泸州市  646000
  • 收稿日期:2017-01-24 出版日期:2017-03-18 发布日期:2017-04-14
  • 通讯作者: 李忠,教授,博士,硕士生导师,主要从事关节软骨损伤与修复的基础与临床研究。
  • 作者简介:刘俊才,男,1982年生,四川省广安市人,硕士,讲师,主要从事关节软骨损伤与修复的基础与临床研究。
  • 基金资助:

    泸州市科技局重点项目(2013-S-48)

Differential expression of integrin alpha5beta1 in the degenerated articular cartilage after knee osteoarthritis

Liu Jun-cai1, Chen You-xia2, Li Zhong1, Wu Shao-jun1, Zuo Yin-long1, Li Wen1, He Ke1, Zhang Zhi-wei1, Li Ren-ming1   

  1. 1Department of Bone and Joint Surgery, 2Department of ICU, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2017-01-24 Online:2017-03-18 Published:2017-04-14
  • Contact: Li Zhong, M.D., Professor, Master’s supervisor, Department of Bone and Joint Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Liu Jun-cai, Master, Lecturer, Department of Bone and Joint Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the Key Project of the Office of Science and Technology of Luzhou, No. 2013-S-48

摘要:

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文题释义:
整合素(Integrin,又译为整联蛋白):是一种介导细胞和其外环境(如细胞外基质)之间连接的跨膜受体。在信号转导中,整合素将细胞外基质的化学成分与力学状态等有关信息传入细胞。因此,整合素除了穿过膜的机械作用,也参与了细胞讯息、细胞周期之调节、细胞型态以及细胞的运动。
细胞外基质:是由动物细胞合成并分泌到胞外、分布在细胞表面或细胞之间的大分子,主要是一些多糖和蛋白,或蛋白聚糖。这些物质构成复杂的网架结构,支持并连接组织结构、调节组织的发生和细胞的生理活动。细胞外基质是动物组织的一部分,不属于任何细胞,它决定结缔组织的特性,对于一些动物组织的细胞具有重要作用。
摘要
背景:
有研究提示整合素α5β1可能与发育性髋关节发育不良髋臼软骨的退变相关。
目的:观察整合素α5β1在膝骨关节炎不同退变程度关节软骨中的表达情况。
方法:选取全膝关节置换患者股骨内外髁负重面不同退变程度关节软骨(75个标本),结合Bobinac分期,将软骨标本分为4组,正常组(6个标本)、轻度退变组(24个标本)、中度退变组(26个标本)、重度退变组(19个标本)。采用Real-time PCR、免疫组织化学及Western blot分别检测整合素α5β1在4组标本中的表达情况。
结果与结论:①各种检测方法结果一致,整合素α5β1在4组标本中均有表达,正常组呈低表达,轻、中度退变组表达逐渐增强,重度退变组表达较前3组明显增强,组间差异有显著性意义(P < 0.05);②结果说明,整合素α5β1在膝骨关节炎不同退变程度关节软骨中存在差异性表达;关节软骨退变越重,整合素α5β1在软骨中的表达越强。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0002-1941-7995(刘俊才)

关键词: 组织构建, 软骨组织工程, 整合素α5β1, 骨关节炎, 膝骨关节炎, 整合素, 关节软骨

Abstract:

BACKGROUND: Integrin α5β1 has been shown to be related to acetabular cartilage degeneration due to developmental dysplasia of the hip.
OBJECTIVE: To investigate the expression of integrin α5β1 in differently degenerated cartilage tissues of the knee after knee osteoarthritis.
METHODS: Seventy-five degenerated articular cartilage specimens at the weight-bearing surface of femoral lateral and medial condyles were removed from patients with knee osteoarthritis who underwent total knee replacement, and were then allotted to four groups based on Mankin’s grading system: normal (n=6), mild degeneration (n=24), moderate degeneration (n=26) and severe degeneration (n=19) groups. The expression of integrin α5β1 in each group was detected using real-time PCR, immunohistochemistry and western blot assay.
RESULTS AND CONCLUSION: The detected results were consistent in the three kinds of assays, and the expression level of integrin α5β1 was ranked as follows: severe degeneration group > moderate degeneration group > mild degeneration group > normal group (P < 0.05). To conclude, differential expression levels of integrin α5β1 are found in different degenerative degrees of articular cartilage in knee osteoarthritis. Furthermore, the expression level of integrin α5β1 is increased with the degenerative severity.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Integrin alpha5beta1, Osteoarthritis, Osteoarthritis, Knee, Cartilage, Articular, Tissue Engineering

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