中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (16): 4054-4066.doi: 10.12307/2026.715

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

脑瘫饮改善雌雄幼鼠脑瘫：基于“肠-脑-肌”轴的作用机制

邹玉雄1，刘晓蒙1，刘  英2，朱  玥1，李书明1，郭芳阳1，庾馨予1，聂鹤云1，刘  潜1，2，敖梅英1   

  1. 1江西中医药大学中医学院，中西医结合一级学科，中西医结合儿童健康研究院，江西省南昌市  330004；2江西中医药大学附属医院，江西省南昌市  330000


  • 收稿日期:2025-03-27 接受日期:2025-08-29 出版日期:2026-06-08 发布日期:2025-11-26
  • 通讯作者: 敖梅英，博士，教授，江西中医药大学中医学院，中西医结合一级学科，中西医结合儿童健康研究院，江西省南昌市 330004 共同通讯作者：刘潜，博士，博士生导师，教授，江西中医药大学中医学院，中西医结合一级学科，中西医结合儿童健康研究院，江西省南昌市 330004；江西中医药大学附属医院，江西省南昌市 330000
  • 作者简介:邹玉雄，男，1998年生，江西中医药大学在读硕士，主要从事中西医结合基础研究。
  • 基金资助:
    国家自然科学基金项目(82060658)，项目负责人：敖梅英；全国重点实验室自主部署重点研发计划项目(20243BCC31008)，项目负责人：刘潜；江西中医药大学科研培育项目(2023ZDPY001)，项目负责人：刘潜；中西医结合防治儿童疾病及健康保障创新研究团队(CXTD22014)，项目负责人：刘潜；江西中医药大学校级教改项目(2022jzyx-2)，项目负责人：敖梅英

Cerebral palsy decoction improves cerebral palsy in male and female young rats: mechanisms based on the “gut-brain-muscle” axis

Zou Yuxiong1, Liu Xiaomeng1, Liu Ying2, Zhu Yue1, Li Shuming1, Guo Fangyang1, Yu Xinyu1, Nie Heyun1, Liu Qian1, 2, Ao Meiying1   

  1. 1Discipline of Chinese and Western Integrative Medicine, Integrated Chinese and Western Medicine Institute for Children Health & Drug Innovation, Jiangxi University of Chinese Medicine, Nanchang 330004, Jiangxi Province, China; 2Affiliated Hospital of Jiangxi University of Chinese Medicine, Nanchang 330000, Jiangxi Province, China
  • Received:2025-03-27 Accepted:2025-08-29 Online:2026-06-08 Published:2025-11-26
  • Contact: Ao Meiying, PhD, Professor, Discipline of Chinese and Western Integrative Medicine, Integrated Chinese and Western Medicine Institute for Children Health & Drug Innovation, Jiangxi University of Chinese Medicine, Nanchang 330004, Jiangxi Province, China Co-corresponding author: Liu Qian, PhD, Doctoral supervisor, Professor, Discipline of Chinese and Western Integrative Medicine, Integrated Chinese and Western Medicine Institute for Children Health & Drug Innovation, Jiangxi University of Chinese Medicine, Nanchang 330004, Jiangxi Province, China; Affiliated Hospital of Jiangxi University of Chinese Medicine, Nanchang 330000, Jiangxi Province, China
  • About author:Zou Yuxiong, MS candidate, Discipline of Chinese and Western Integrative Medicine, Integrated Chinese and Western Medicine Institute for Children Health & Drug Innovation, Jiangxi University of Chinese Medicine, Nanchang 330004, Jiangxi Province, China
  • Supported by:
    National Natural Science Foundation of China, No. 82060658 (to AMY); Key Research and Development Program of National Key Laboratories, No. 20243BCC31008 (to LQ); Jiangxi University of Chinese Medicine Scientific Research Cultivation Project, No. 2023ZDPY001 (to LQ); Innovative Research Team on Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Pediatric Diseases and Health Protection, No. CXTD22014 (to LQ); Jiangxi University of Chinese Medicine Institutional-Level Teaching Reform Project, No. 2022jzyx-2 (to AMY)

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摘要：


文题释义：
脑瘫：是一种非进行性的神经系统疾病，主要表现为运动功能障碍和姿势异常。它是由大脑在发育过程中受到损伤或发育异常引起的，通常发生在出生前、出生时或出生后的早期阶段。脑瘫的预后因病情严重程度和干预时机而异。早期诊断和干预可以显著改善患儿的功能和生活质量。尽管脑瘫无法完全治愈，但通过综合治疗，许多患儿能够获得较好的独立生活能力。
“肠-脑-肌”轴：目前已有大量研究证实了“脑-肠轴”与“肌-肠轴”的存在，而研究发现肠道、大脑和肌肉三者之间也存在密切联系。基于这一发现，此研究以脑瘫幼鼠为模型，深入探究肠道菌群的变化与脑组织及肌肉组织之间的潜在关联。

背景：基于江西省中医院20多年脑瘫饮的临床用药经验，已证实脑瘫饮治疗脑瘫的有效性及安全性，但是作用机制尚未明确。
目的：探讨脑瘫饮对雌雄脑瘫幼鼠肠道菌群、神经细胞与神经递质、运动功能障碍的作用及关联性。
方法：采用宫内感染与缺氧处理联合出生后运动限制构建SD幼鼠脑瘫模型，模型组与给药组在造模后分别灌胃生理盐水与脑瘫饮，给药15 d。通过悬吊实验与斜坡实验检测行为学，苏木精-伊红染色和尼氏染色观察脑组织病理变化，ELISA法检测神经递质水平，16S rDNA高通量测序肠道菌群。
结果与结论：①与模型组相比，给药组斜坡和悬吊行为学能力增强(P < 0.01)；②与模型组相比，给药组海马体、大脑皮质中神经元细胞和尼氏小体数量增多；③在海马体中，与模型组相比，给药组乙酰胆碱水平升高(雄鼠：P < 0.05；雌鼠：P > 0.05)，乙酰胆碱酶水平降低(雄鼠：P < 0.01；雌鼠：P < 0.01)，去甲肾上腺素水平升高(雄鼠：P < 0.05；雌鼠：P < 0.001)，5-羟色胺水平升高(雄鼠：P < 0.05；雌鼠：P > 0.05)；④在大脑皮质中，与模型组相比，给药组乙酰胆碱水平升高(雄鼠：P < 0.05；雌鼠：P > 0.05)，乙酰胆碱酶水平降低(雄鼠：P < 0.05；雌鼠：P > 0.05)，去甲肾上腺素水平升高(雄鼠：P < 0.01；雌鼠：P < 0.05)，5-羟色胺水平升高(雄鼠：P < 0.01；雌鼠：P < 0.01)；⑤与模型组相比，给药组肠道菌群中norank_f__Oscillospiraceae属、大肠杆菌属、norank_f__Muribaculaceae属与乳酸杆菌属菌群的种类与丰度显著改善；脑瘫饮所改善肠道菌群的种类与丰度在雌雄鼠之间呈现显著性差异。由此可知，临床经验方脑瘫饮可以通过调整肠道菌群结构与丰度，促进脑组织神经元与尼氏小体的发育、影响神经递质水平，进而有效改善脑瘫幼鼠的运动功能障碍，形成了“肠-脑-肌”轴关联。
https://orcid.org/0009-0007-5340-8842(邹玉雄)；https://orcid.org/0000-0001-7633-9172(刘潜)；https://orcid.org/0000-0003-4502-1772(敖梅英)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 脑瘫, 脑瘫饮, 雌雄差异, 海马体, 大脑皮质, 神经递质, 肠道菌群, 16S rDNA

Abstract: BACKGROUND: Based on over 20 years of clinical experience with cerebral palsy decoction at Jiangxi Provincial Hospital of Traditional Chinese Medicine, its efficacy and safety have been confirmed in the treatment of cerebral palsy, but the underlying mechanisms remain unclear.
OBJECTIVE: To investigate the effects and correlations of cerebral palsy decoction on gut microbiota, neural cells and neurotransmitters, as well as motor dysfunction in both female and male young rats with cerebral palsy.
METHODS: The cerebral palsy model was prepared in Sprague-Dawley young rats subjected to intrauterine infection and hypoxia combined with postnatal movement restriction. After modeling, the model group and the administration group were administered normal saline and cerebral palsy decoction via gavage, respectively. Administration in each group lasted 15 days. Behavioral performance was assessed using the suspension test and slope test. Pathological changes in brain tissue were observed through hematoxylin-eosin staining and Nissl staining. Neurotransmitter levels were measured using ELISA, and gut microbiota was analyzed by 16S rDNA high-throughput sequencing.
RESULTS AND CONCLUSION: (1) Compared with the model group, the administration group showed enhanced behavioral abilities in slope and suspension tests (P < 0.01). (2) Compared with the model group, the number of neuronal cells and Nissl bodies in the hippocampus and cerebral cortex increased in the administration group. (3) In the hippocampus, compared with the model group, the administration group exhibited elevated acetylcholine levels (male: P < 0.05; female: P > 0.05), decreased acetylcholinesterase levels (male: P < 0.01; female: P < 0.01), increased norepinephrine levels (male: P < 0.05; female: P < 0.001), and elevated serotonin levels (male: P < 0.05; female: P > 0.05). (4) In the cerebral cortex, compared with the model group, the administration group showed increased acetylcholine levels (male: P < 0.05; female: P > 0.05), decreased acetylcholinesterase levels (male: P < 0.05; female: P > 0.05), elevated norepinephrine levels (male: P < 0.01; female: P < 0.05), and increased serotonin levels (male: P < 0.01; female: P < 0.01). (5) Compared with the model group, the administration group demonstrated significant improvements in the diversity and abundance of gut microbiota, including the genera norank_f__Oscillospiraceae, Escherichia, norank_f__Muribaculaceae, and Lactobacillus. The improvements in gut microbiota diversity and abundance showed significant differences between male and female rats. These findings indicate that the clinical empirical formula, cerebral palsy decoction, can effectively improve motor dysfunction in young rats with cerebral palsy by modulating the structure and abundance of gut microbiota, promoting the development of neurons and Nissl bodies in brain tissues, and influencing neurotransmitter levels. This establishes a “gut-brain-muscle” axis association.


Key words: cerebral palsy, cerebral palsy decoction, male-female differences, hippocampus, cerebral cortex, neurotransmitter, gut microbiota, 16S rDNA

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