中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (31): 5007-5012.doi: 10.3969/j.issn.2095-4344.2108

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

氨基葡萄糖胶囊干预膝骨关节炎患者外周血单个核细胞中软骨代谢相关基因的表达

肖  镭，侯立刚   

  1. 郑州大学附属郑州中心医院骨科，河南省郑州市  450000
  • 收稿日期:2019-10-18 修回日期:2019-10-26 接受日期:2019-11-25 出版日期:2020-11-08 发布日期:2020-09-04
  • 通讯作者: 肖镭，副主任医师，郑州大学附属郑州中心医院骨科，河南省郑州市 450000
  • 作者简介:肖镭，男，1979年生，汉族，副主任医师，主要从事创伤、关节方面的研究。
  • 基金资助:
    2018年河南省科技攻关计划(182102310531)

Effect of glucosamine capsule on cartilage metabolism-related genes in peripheral blood mononuclear cells of patients with knee osteoarthritis

Xiao Lei, Hou Ligang   

  1. Department of Orthopedics, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Received:2019-10-18 Revised:2019-10-26 Accepted:2019-11-25 Online:2020-11-08 Published:2020-09-04
  • Contact: Xiao Lei, Associate chief physician, Department of Orthopedics, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • About author:Xiao Lei, Associate chief physician, Department of Orthopedics, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Supported by:
    the Scientific and Technological Research Plan of Henan Province in 2018, No. 182102310531

PDF

136

摘要：

文题释义：

膝骨关节炎：是由膝关节软骨退行性改变引起的慢性关节疾病，以关节软骨受损、软骨下骨板及关节边缘骨病理性改变为特征，表现为膝关节疼痛、僵硬、运动受限等症状，常呈慢性进行性发展。

软骨细胞代谢相关基因：软骨细胞是软骨组织中唯一的细胞类型，其合成和分解代谢平衡是维持软骨正常功能的前提；软骨细胞存活及凋亡受多种基因调控，在膝骨关节炎发病过程中起关键作用，研究治疗膝骨关节炎药物对软骨细胞代谢基因的调控作用有重要意义。

背景：目前关于氨基葡萄糖的研究多集中于对膝骨关节炎的治疗作用，但关于其对膝骨关节炎患者外周血中软骨代谢相关基因影响的研究有限。

目的：观察氨基葡萄糖胶囊对膝骨关节炎的治疗效果及对软骨代谢相关基因表达的影响。

方法：选取2017年3月至2019年2月郑州大学附属郑州中心医院收治的90例膝骨关节炎患者，另选取同期医院体检中心收入的40例健康受试者，检测并对比健康受试者与膝骨关节炎患者治疗前外周血单个核细胞中软骨寡聚基质蛋白、Ⅱ型胶原纤维α1、聚糖蛋白、特异性组织抑制物3基因表达水平。采用随机数表法将膝骨关节炎患者分为常规组和研究组，分别给予双氯芬酸钠缓释片、双氯芬酸钠缓释片+氨基葡萄糖胶囊治疗12周，对比治疗前后2组外周血单个核细胞中各基因表达水平、Lequesne指数，统计治疗期间2组不良反应发生情况。

结果与结论：①与健康受试者比较，膝骨关节炎患者外周血单个核细胞中软骨寡聚基质蛋白、特异性组织抑制物3 mRNA相对表达量升高(P < 0.05)，Ⅱ型胶原纤维α1、聚糖蛋白mRNA相对表达量降低(P < 0.05)；②治疗前研究组和常规组外周血单个核细胞中各基因表达水平比较，差异均无显著性意义(P > 0.05)；治疗后2组外周血单个核细胞中软骨寡聚基质蛋白、特异性组织抑制物3 mRNA相对表达量均低于治疗前，且研究组低于常规组(P < 0.05)；治疗后2组外周血单个核细胞中Ⅱ型胶原纤维α1、聚糖蛋白mRNA相对表达量均高于治疗前，且研究组高于常规组(P < 0.05)；③治疗前研究组和常规组Lequesne指数比较，差异均无显著性意义(P > 0.05)；治疗后2组Lequesne指数均低于治疗前，且研究组低于常规组(P < 0.05)；④研究组和常规组不良反应发生率比较，差异均无显著性意义(P > 0.05)；⑤结果表明，氨基葡萄糖胶囊可有效改善膝骨关节炎患者临床症状且安全可靠，可能通过抑制软骨寡聚基质蛋白、特异性组织抑制物3基因表达，促进Ⅱ型胶原纤维α1、聚糖蛋白基因表达实现的。

ORCID: 0000-0002-2934-0725(肖镭)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 氨基葡萄糖胶囊, 膝骨关节炎,  外周血单个核细胞,  软骨寡聚基质蛋白,  Ⅱ型胶原纤维α1基因,  聚糖蛋白,  特异性组织抑制物3

Abstract:

BACKGROUND: At present, the research on glucosamine mostly focused on the treatment of knee osteoarthritis, but the research on the effect of glucosamine on cartilage metabolism-related genes in peripheral blood of patients with knee osteoarthritis is limited.

OBJECTIVE: To observe the effect of glucosamine capsule on knee osteoarthritis and the expressions of cartilage metabolism-related genes.  

METHODS: Totally 90 knee osteoarthritis patients admitted to the Zhengzhou Central Hospital Affiliated to Zhengzhou University from March 2017 to February 2019 were selected, and 40 healthy subjects from the medical examination center in the same period were selected as healthy subjects. The cartilage oligomeric matrix protein (COMP), type II collagen fiber α1 gene (COL2A1), glycoprotein and specific tissue inhibitor (TIMP)-3 gene expression levels in peripheral blood mononuclear cells were detected and compared between healthy subjects and knee osteoarthritis patients before treatment. Knee osteoarthritis patients were divided into routine group and study group by random number table, and they were treated with diclofenac sodium sustained-release tablets, diclofenac sodium sustained-release tablets and glucosamine capsules, respectively. Both groups were treated for 12 weeks. The levels of gene expressions and Lequesne index in peripheral blood mononuclear cells of two groups were compared before and after treatment. During the treatment, the occurrence of adverse reactions was counted in the two groups.

RESULTS AND CONCLUSION: (1) The relative expressions of COMP and TIMP-3 in peripheral blood mononuclear cells of knee osteoarthritis patients were higher than those of healthy subjects (P < 0.05), while the mRNA relative expressions of COL2A1 and glycoprotein were lower than those of healthy subjects (P < 0.05). (2) There were no significant difference in gene expression levels in peripheral blood mononuclear cells before treatment between study group and routine group (P > 0.05). The mRNA relative expressions of COMP and TIMP-3 in peripheral blood mononuclear cells of the two groups after treatment were lower than those before treatment, and those of the study group were lower than those of the routine group (P < 0.05). The mRNA relative expressions of COL2A1 and glycoprotein in peripheral blood mononuclear cells of the two groups after treatment were higher than those before treatment, and the mRNA relative expressions of COL2A1 and ACAN in the study group were higher than those in the routine group (P < 0.05). (3) There was no significant difference in Lequesne index between the study group and the routine group before treatment (P > 0.05). Lequesne index in both groups after treatment was lower than that before treatment, and that in the study group was lower than that in the routine group (P < 0.05). (4) There was no significant difference in the incidence of adverse reactions between the study group and the routine group (P > 0.05). (5) Results suggest that glucosamine capsule can effectively improve the clinical symptoms of patients with knee osteoarthritis. It is safe and reliable. It may promote the expression of COL2A1 and glycoprotein genes by inhibiting the expression of COMP and TIMP-3 genes.

Key words: glucosamine capsule,  knee osteoarthritis,  peripheral blood mononuclear cells,  cartilage oligomeric matrix protein,  type II collagen fiber α1 gene,  glycoprotein,  specific tissue inhibitor-3

中图分类号: