中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (34): 5469-5476.doi: 10.3969/j.issn.2095-4344.0682

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

等离子体电解氧化涂层钛金属植入物体内骨整合的评价

贺 韬1，2，张云辉3，张 超1，徐志国4，董宇启1   

  1. 1上海交通大学医学院附属仁济医院创伤骨科，上海市 200127；2德国慕尼黑大学附属大哈登医院骨科，生物力学与组织工程实验室，德国慕尼黑 81377；3上海市宝山区中西医结合医院骨科，上海市 201900；4山东能源枣矿集团中心医院骨科，山东省枣庄市 277100
  • 收稿日期:2018-09-02 出版日期:2018-12-08 发布日期:2018-12-08
  • 通讯作者: 董宇启，主任医师，上海交通大学医学院附属仁济医院创伤骨科，上海市 200127
  • 作者简介:贺韬，男，1987年生，浙江省宁波市人，汉族，慕尼黑大学医学院在读博士，医师，主要从事骨与软骨组织工程、植入物骨整合、骨质疏松性骨折研究。
  • 基金资助:

    国家自然科学基金项目(30700170)；上海市科委基础重点项目(07JC14057)；上海市科委纳米专项项目(0852nm03300)；中国国家留学基金管理委员会项目(201606230235)

Evaluation of osseointegration of plasma electrolytic oxidation coated titanium implants in vivo

He Tao1, 2, Zhang Yunhui3, Zhang Chao1, Xu Zhiguo4, Dong Yuqi1   

  1. 1Department of Traumatic Orthopedics, Shanghai Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; 2Laboratory of Biomechanics and Experimental Orthopaedics, Clinic Großhadern, Ludwig-Maximillian University, Munich 81377, Germany; 3Department of Orthopedics, Shanghai Baoshan District Hospital of Integrated Traditional and Western Medicine, Shanghai 201900, China; 4Department of Orthopedics, Shandong Energy Zaozhuang Mining Central Hospital, Zaozhuang 277100, Shandong Province, China
  • Received:2018-09-02 Online:2018-12-08 Published:2018-12-08
  • Contact: Dong Yuqi, Chief surgeon, Department of Traumatic Orthopedics, Shanghai Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
  • About author:He Tao, Doctorate candidate, Surgeon, Department of Traumatic Orthopedics, Shanghai Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; Laboratory of Biomechanics and Experimental Orthopaedics, Clinic Gro?hadern, Ludwig-Maximillian University, Munich 81377, Germany
  • Supported by:

    the National Natural Science Foundation of China, No. 30700170; Shanghai Key Basic Project of Shanghai Science and Technology Commission, No. 07JC14057; Shanghai Science and Technology Commission Nano Special Project, No. 0852nm03300; the Funded Project of China Scholarship Council, No. 201606230235

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摘要：

文章快速阅读：

 

文题释义：
等离子体电解氧化涂层：是一种新型原位涂层技术，其与金属植入物基体结合强度高，不易剥脱，形成微颗粒，引起植入物周围炎症反应，进而导致植入物松动失效。同时，该涂层可在植入物表面形成多孔纳米结构，提供了良好的生物活性和更强的与周围骨组织结合的能力，是一种具有良好临床应用价值的涂层技术。
骨整合：即植入物与具有活性的骨组织产生持久性的骨性接触而界面无纤维组织介入的过程，其反映了植入物与周围正常骨组织是否产生了有效结合。因此，骨整合是临床与科研中评价植入物是否成功的金标准。通过对新型涂层植入物的骨整合效果进行体内实验评价，可有效评估该涂层是否具有良好的生物活性及是否有望投入临床应用。
 
 
背景：多项体外细胞学实验显示，含氧化涂层钛金属较普通钛金属表面表现出更好的生物相容性和生物活性。
目的：进一步观察等离子体电解氧化涂层钛棒在动物体内的骨整合能力。

方法：取36只16周龄雄性SD大鼠(购自上海西普尔-必凯实验动物有限公司)，建立双侧股骨远端髓内钉植入模型，左侧植入多孔纳米等离子体电解氧化涂层钛棒，作为实验组；右侧植入无涂层钛棒，作为对照组。植入2，4，6周时，处死大鼠并收获股骨远端带有金属植入物的骨组织标本，进行Van Gieson染色、双标荧光染色及生物力学评价。

结果与结论：①Van Gieson染色显示，实验组与对照组植入物-骨组织界面均未见纤维组织长入；植入2周时，两组均可见植入物表面部分骨组织接触，实验组于植入4周时形成植入物周围骨整合，对照组于植入6周时形成植入物周围骨整合；②双标荧光染色显示，植入2周时，两组植入物周围均产生大量新生骨组织，与钛棒结合紧密；植入4周时，实验组新生骨与植入物表面呈现连续性接触，对照组新生骨与植入物表面呈不连续区块接触；植入6周时，两组均显示出了连续的植入物-骨组织接触，但新生骨骨量明显较4周时减少；③生物力学检测显示，实验组植入4周时的最大压缩载荷高于对照组(P < 0.001)，两组植入2，6周时的最大压缩载荷比较无差异(P > 0.05)；实验组植入4周时的最大压缩载荷高于植入2，6周时(P < 0.05)，对照组植入6周时的最大压缩载荷高于植入2，4周时(P < 0.05)；④结果表明，等离子体电解氧化涂层钛棒植入早期的骨整合能力优于普通钛棒。

ORCID: 0000-0001-7858-0971(张云辉)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 等离子体电解氧化涂层, 钛金属, 植入物, 骨整合, 双标荧光, 生物力学, 逆行股骨髓内钉模型, 体内实验, 生物材料

Abstract:

BACKGROUND: A number of in vitro cytological experiments have shown that titanium oxide surfaces with oxidized coatings exhibit better biocompatibility and biological activity than conventional titanium metal surfaces.

OBJECTIVE: To further observe the osseointegration ability of plasma electrolytic oxidation (PEO) coated titanium rods in vivo.
METHODS: Thirty-six 16-week-old male Sprague-Dawley rats (purchased from Shanghai Sippr-BK Laboratory Animal Co., Ltd., China) were used to establish a bilateral distal femoral intramedullary nail implantation model. PEO coated titanium rods were implanted into the left side as the PEO group, whilst uncoated titanium rods were implanted into the right side as the control group. At 2, 4, and 6 weeks after implantation, the rats were sacrificed and bone specimens with implants at the distal femur were harvested. Van Gieson staining, double-labeled fluorescent staining, and biomechanical evaluation were then performed.
RESULTS AND CONCLUSION: (1) Van Gieson staining results showed no fibrous tissue ingrowth at the implant-bone interface in both the experimental group and the control group. At 2 weeks of implantation, bone tissue growth was observed at the implant surface in both groups. The osseointegration around the implant was formed at 4 weeks of implantation in the PEO group, while that of control group was formed at 6 weeks after implantation. (2) Double-labeled fluorescent staining results showed that at 2 weeks after implantation, implants in both groups were closely bound to a large amount of newly formed bone tissue. When implanted for 4 weeks, the newly formed bone in PEO group was in continuous contact with the surface of the implant, while that in the control group was in contact with the surface of the implant in a discontinuous block. At 6 weeks after implantation, continuous implant-bone contact was observed in both groups, but the amount of newly formed bone was significantly lower than that at 4 weeks. (3) Biomechanical examination results showed that the maximum compression load of the PEO group at 4 weeks was higher than that of the control group (P < 0.001). There was no significant difference in the maximum compression load between the two groups at 2 and 6 weeks (P > 0.05). In the PEO group, the maximum compression load at 4 weeks after implantation was higher than that at 2 and 6 weeks after implantation (P < 0.05), while in the control group, the maximum compression load at 6 weeks after implantation was higher than that at 2 and 4 weeks after implantation (P < 0.05). Overall, our study findings indicate that the PEO coated titanium rods have better osseointegration than pure titanium rods in the early stage after implantation.  

Key words: Titanium, Prostheses and Implants, Osseointegration, Biomechanics, Tissue Engineering

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