中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8671-8674.doi: 10.3969/j.issn.2095-4344.2012.46.024

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

EphB3基因受体蛋白在大鼠脊髓损伤组织中的表达

吴 刚，吕 刚，智晓东   

  1. 辽宁医学院附属第一医院骨科，辽宁省锦州市 121001
  • 收稿日期:2012-03-26 修回日期:2012-05-23 出版日期:2012-11-11 发布日期:2013-03-16
  • 通讯作者: 吕刚，博士生导师，辽宁医学院附属第一医院骨科，辽宁省锦州市 121001
  • 作者简介:吴刚★，男，1982年生，辽宁省锦州市人，汉族，辽宁医学院在读硕士，主要从事骨外科脊髓损伤修复再生研究。 1304271818@qq.com

Expression of EphB3 receptor protein in injured spinal cord tissues of rats

Wu Gang, Lü Gang, Zhi Xiao-dong   

  1. Department of Orthopedics, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Received:2012-03-26 Revised:2012-05-23 Online:2012-11-11 Published:2013-03-16
  • Contact: Lü Gang, Doctoral supervisor, Department of Orthopedics, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • About author:Wu Gang, Studying for master’s degree, Department of Orthopedics, First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China 1304271818@qq.com

PDF

510

被引次数

7

摘要：

背景：Eph受体能够调节轴突介导，形成抑制轴突修复再生的内环境，而EphB3又是Eph家族中极其重要的一员，所以研究EphB3与脊髓损伤的关系将成为国内外研究的新方向。
目的：观察脊髓损伤大鼠EphB3基因和蛋白的表达情况。
方法：采用脊髓半切法建立大鼠脊髓损伤模型，RT-PCR和Western blot法检测脊髓损伤后1，2，4，8周脊髓组织中EphB3 mRNA及蛋白的表达，并且与正常大鼠进行比较。
结果与结论：损伤组脊髓组织中EphB3 mRNA及蛋白呈高表达，且1，2，4，8周时间点EphB3 mRNA及蛋白表达无明显变化(P ≥ 0.05)。对照组脊髓组织中EphB3 mRNA及蛋白表达极低。两组比较差异有显著性意义(P < 0.05)。结果提示脊髓损伤引起EphB3基因和蛋白呈长时间稳定的高表达。

关键词: 脊髓损伤, 修复再生, EphB3, mRNA, 蛋白

Abstract:

BACKGROUND: Eph receptors can regulate axon-mediated environment inhibiting axonal repair and regeneration. EphB3 is an extremely important member of the Eph family, therefore, to study the relationship between EphB3 and spinal cord injury will become the new direction of research at home and abroad.
OBJECTIVE: To observe the expression of EphB3 receptor protein and gene in rats with spinal cord injury.
METHODS: The spinal cord hemisection method was used to establish a rat model of spinal cord injury. Using reverse transcription-PCR and Western blot assay, we observed the mRNA and protein expressions of EphB3 in the spinal cord at 1, 2, 4 and 8 weeks after spinal cord injury in comparison with normal rats.
RESULTS AND CONCLUSION: EphB3 gene and protein were highly expressed in injured spinal cord tissues, but they had no significant changes at 1, 2, 4 and 8 weeks after injury (P ≥ 0.05). In the spinal cord tissues of normal rats, the mRNA and protein expressions of EphB3 were very low. A significant difference in the mRNA and protein expressions was found between spinal cord injury rats and normal rats (P < 0.05). These findings indicate that spinal cord injury can result in the long-term, stable and high expression of EphB3 receptor protein.

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