中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8506-8509.doi: 10.3969/j.issn.2095-4344.2012.45.027

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

不同年龄段大鼠脊髓源性神经干细胞的体外培养及鉴定

于滋超，郭开今   

  1. 徐州医学院附属医院骨科，江苏省徐州市 221000
  • 收稿日期:2012-02-01 修回日期:2012-02-22 出版日期:2012-11-04 发布日期:2013-03-16
  • 通讯作者: 郭开今，主任医师，教授，博士，硕士生导师，徐州医学院附属医院骨科，江苏省徐州市 221000 XZGKJ@sina.com
  • 作者简介:于滋超★，男，1985年生，山东省即墨市人，汉族，徐州医学院在读硕士，主要从事脊柱外科的研究。 yuzichao001@163.com

In vitro culture and identification of spinal cord neural stem cells from rats of different ages

Yu Zi-chao, Guo Kai-jin   

  1. Department of Orthopedics, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China
  • Received:2012-02-01 Revised:2012-02-22 Online:2012-11-04 Published:2013-03-16
  • Contact: Guo Kai-jin, M.D., Chief physician, Professor, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China XZGKJ@sina.com
  • About author:Yu Zi-chao★, Studying for master’s degree, Department of Orthopedics, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221000, Jiangsu Province, China yuzichao001@163.com

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538

被引次数

2

摘要：

背景：不同年龄段神经干细胞的增殖及分化特性目前未见报道。
目的：观察不同年龄段大鼠脊髓源性神经干细胞的体外分离、培养及鉴定。
方法：取不同年龄大鼠的脊髓，制成单细胞悬液，用含有表皮生长因子、碱性成纤维细胞生长因子和B27的DMEM/F12培养基，以细胞浓度5.0×105 L-1进行原代培养，每隔3 d传代1次。
结果与结论：不同年龄段大鼠的脊髓中均可培养出细胞，其形态为结构紧密、大小不等的悬浮细胞球，称之为“神经干细胞球”；经过传代后单细胞又迅速增殖成球且数目明显增多。提示不同年龄段大鼠脊髓中均能够在体外培养出神经细胞球。

关键词: 脊髓, 神经干细胞, 细胞培养, 鉴定, 大鼠, 年龄, 干细胞

Abstract:

BACKGROUND: There have been few reports describing the proliferation and differentiation characteristics of neural stem cells from rats of different ages.
OBJECTIVE: To investigate the in vitro isolation, culture and identification of spinal cord neural stem cells from rats of different ages.
METHODS: Spinal cord from rats of different ages was taken and prepared into single cell suspension. Primary cells at 5.0×105/L were cultured with DMEM/F12 medium containing epidermal growth factor, basic fibroblast growth and B27 and then passaged once every other day.
RESULTS AND CONCLUSION: Cells could be cultured from different ages of rat spinal cord. The suspending cell spheres, named neural stem cell spheres showed compact structure and were of different sizes. After passage, single cells proliferated rapidly and form neurospheres and the number increased obviously. Neural stem cell spheres can be in vitro cultured from the spinal cord of rats at different ages.

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