中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (38): 7101-7106.doi: 10.3969/j.issn.2095-4344.2012.38.015

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

微弧氧化AZ31镁合金的生物相容性

王树峰1，李春荣2，王程越3，李淑华4，谭丽丽5，杨 柯5   

  1. 1辽宁医学院研究生学院,辽宁省锦州市 121000
    2新疆裕民县人民医院口腔科，新疆维吾尔自治区裕民县 834800
    3辽宁医学院第五临床医院，辽宁省锦州市 121000
    4辽宁医学院免疫与微生物学实验室，辽宁省锦州市 121000
    5中国科学院金属研究所，辽宁省沈阳市 110016
  • 收稿日期:2012-01-04 修回日期:2012-02-11 出版日期:2012-09-16 发布日期:2012-09-16
  • 通讯作者: 王程越，博士，硕士生导师，辽宁医学院第五临床医院，辽宁省锦州市 121000 wchy1758@ sohu.com
  • 作者简介:王树峰，男，1986年生，山东省潍坊市人，汉族，2010年济宁医学院毕业，医师，主要从事口腔修复专业的研究。 wsf_20052005@163.com

Biocompatibility of AZ31 magnesium alloy treated with micro-arc oxidation

Wang Shu-feng1, Li Chun-rong2, Wang Cheng-yue3, Li Shu-hua4, Tan Li-li5, Yang Ke5   

  1. 1Graduate School of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
    2Depatment of Stomatology, Yumin People’s Hospital, Yumin 834800, Xinjiang Uyghur Autonomous Region, China
    3the Fifth Clinical Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
    4the Immune and Microbiology Laboratory of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China
    5Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, Liaoning Province, China
  • Received:2012-01-04 Revised:2012-02-11 Online:2012-09-16 Published:2012-09-16
  • Contact: Wang Cheng-yue, Doctor, Master’s supervisor, the Fifth Clinical Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China wchy1758@ sohu.com
  • About author:Wang Shu-feng, Physician, Graduate School of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China wsf_20052005@ 163.com

PDF

595

被引次数

16

摘要：

背景：表面改性技术和合金成分的变化都会影响到镁合金的生物特性。
目的：探讨微弧氧化处理AZ31镁合金的生物相容性。
方法：将体外扩增培养的兔第3代骨髓基质细胞接种到未处理AZ31镁合金(镁合金+骨髓基质细胞组)和微弧氧化AZ31镁合金(微弧氧化镁合金+骨髓基质细胞组)材料上，另设骨髓基质细胞组做对照。培养1，3，5，7 d观察细胞形态，细胞生长状况，细胞黏附状况，材料表面状况。
结果与结论：3组细胞形态一致，以梭形为主。镁合金+骨髓基质细胞组中细胞生长状况明显不如微弧氧化镁合金+骨髓基质细胞组和骨髓基质细胞组(P < 0.05)。微弧氧化镁合金+骨髓基质细胞组材料表面有大量的细胞附着，并且生长正常，镁合金+骨髓基质细胞组材料表面没有细胞附着，有明显的裂痕(P < 0.05)。结果证实，微弧氧化处理AZ31镁合金具有良好的生物相容性，有利于细胞的附着和正常生长。

关键词: 微弧氧化, AZ31镁合金, 骨髓基质细胞, 生物相容性, 组织工程

Abstract:

BACKGROUND: Surface modification and the alteration of alloying components will affect the biocompatibility of magnesium alloy.
OBJECTIVE: To evaluate the biocompatibility of AZ31 magnesium alloy treated with micro-arc oxidation.
METHODS: The passage 3 rabbit bone marrow stroma cells (BMSCs) cultured and expanded in vitro were collected. The cells were implanted into the un-treated AZ31 magnesium alloy (magnesium alloy+BMSCs group) and AZ31 magnesium alloy treated with micro-arc oxidation (magnesium alloy treated with micro-arc oxidation+BMSCs group). In addition, BMSCs were as the blank control group (BMSCs group). Cellular morphology, cell growth and adhesion conditions as well as material surface appearance were observed after cultured for 1, 3, 5 and 7 days.
RESULTS AND CONCLUSION: The cellular morphology in each group was the same, and mainly showed fusiformis. The cell growth condition of the magnesium alloy+BMSCs group was significantly not better than that of magnesium alloy treated with micro-arc oxidation+BMSCs group and BMSCs group (P < 0.05). A large number of cells that grew normally were found adhesion in the material surface of the magnesium alloy treated with micro-arc oxidation+BMSCs group. There were no cells adhered onto the material surface and obvious cracks were seen in the magnesium alloy+BMSCs group. These findings suggest that AZ31 magnesium alloy treated with micro-arc oxidation has good biocompatibility, which is helpful to cell adhesion and normal growth.

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