中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (38): 7042-7046.doi: 10.3969/j.issn.2095-4344.2012.38.003

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米羟基磷灰石/聚己内酯复合大鼠骨髓间充质干细胞的生物相容性

李家锋1，徐金霞2，管海虹3，崔 群2，孙秀英2，陈丽娟3，韩建国2   

  1. 1徐州医学院附属医院口腔颌面外科，江苏省徐州市 221002
    2徐州医学院口腔医学院，江苏省徐州市 221004
    3徐州医学院附属徐州市立医院口腔颌面外科，江苏省徐州市 221002
  • 收稿日期:2012-04-20 修回日期:2012-05-12 出版日期:2012-09-16 发布日期:2012-09-16
  • 通讯作者: 韩建国，硕士，主任医师，徐州医学院口腔医学院，江苏徐州市 221004 hanjg@ xzmc.edu.cn
  • 作者简介:李家锋★，男，1968年生，江苏省徐州市人，汉族，2001年南京医科大学毕业，硕士，硕士生导师，副教授，主任医师，主要从事间充质干细胞复合纳米羟基磷灰石/聚己内酯构建组织工程的实验研究。 JiafengLi203@ 163.com

Biocompatibility of nano hydroxyapatite/polycaprolactone compounded with rat bone marrow mesenchymal stem cells

Li Jia-feng1, Xu Jin-xia2, Guan Hai-hong3, Cui Qun2, Sun Xiu-ying2, Chen Li-juan3, Han Jian-guo2   

  1. 1Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, JIangsu Province, China
    2School of Stomatology, Xuzhou Medical College, Xuzhou 221004, Jiangsu Province, China
    3Department of Oral and Maxillofacial Surgery, Xuzhou Municipal Hospital Affiliated to Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
  • Received:2012-04-20 Revised:2012-05-12 Online:2012-09-16 Published:2012-09-16
  • Contact: Han Jian-guo, Master, Chief physician, School of Stomatology, Xuzhou Medical College, Xuzhou 221004, Jiangsu Province, China hanjg@xzmc.edu.cn
  • About author:Li Jia-feng★, Master, Master’s supervisor, Associate professor, Chief physician, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China JiafengLi203@ 163.com

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555

被引次数

4

摘要：

背景：纳米羟基磷灰石/聚己内酯是一种具有优良生物相容性和生物活性的典型生物复合材料。
目的：分析纳米羟基磷灰石/聚己内酯电纺薄膜作为组织工程骨支架的可行性。
方法：采用静电纺丝技术制备纳米羟基磷灰石/聚己内酯电纺薄膜，将其与第3代SD大鼠骨髓间充质干细胞复合培养，在地塞米松、β-磷酸甘油钠、维生素C成骨诱导剂诱导下，诱导骨髓间充质干细胞向成骨细胞转化。
结果与结论：纳米羟基磷灰石/聚己内酯支架具有合适的微孔结构，且孔道相互贯通。①倒置显微镜观察：复合培养 7 d后细胞大部分为梭形，细胞开始分裂；14 d后，细胞生长比较旺盛，数量明显增多，细胞分泌基质并黏附于支架上。②扫描电镜观察：复合培养7 d后大量细胞位于支架孔隙内生长，增殖良好，细胞大多呈梭形，双极突起，形态较佳，呈立体状生长，并分泌基质，有纤维连接蛋白生成。表明纳米羟基磷灰石/聚己内酯支架具有良好的生物相容性，是骨组织工程的良好载体。

关键词: 纳米羟基磷灰石/聚己内酯支架, 骨髓间充干质细胞, 诱导成骨, 生物相容性, 电纺薄膜

Abstract:

BACKGROUND: Nano hydroxyapatite/polycaprolactone with excellent biocompatibility and biological activity is a typical biological compound material.
OBJECTIVE: To investigate the feasibility of nano hydroxyapatite/polycaprolactone electrospun film as scaffold materials for bone tissue engineering
METHODS: First, nano hydroxyapatite/polycaprolactone electrospun film was prepared by electrostatic spinning technology. Second, the nano hydroxyapatite/polycaprolactone electrospun film was co-cultured in vitro with passage 3 bone marrow mesenchymal stem cells of rats. Then, bone marrow mesenchymal stem cells were induced into osteoblasts by dexamethasone, β-glycerophophate and vitamin C.
RESULTS AND CONCLUSION: Nano hydroxyapatite/polycaprolactone scaffolds possessed good macro porous structure, and pores were interconnected with each other. ①Inverted microscope observation: After bone marrow mesenchymal stem cells and the scaffold were co-cultured for 7 days, cells were mostly fusiform and began to proliferate. After 14 days, cell growth was more vigorous, and the number of cells was increased significantly. Moreover, cells secreted the extracellular matrix and adhered to the scaffold. ②Scanning electron microscope observation: At day 7, a large number of cells grew in scaffold pores, and they had active proliferation capacity. Besides, cells were mostly fusiform, and had good bipolar protrusions. In addition, the cells showed three- dimensional-like growth and secreted the extracellular matrix, as well as fibronectin was produced. These results suggest that the nano hydroxyapatite/polycaprolactone scaffold has good biocompatibility, and it can be used as a good carrier for bone tissue engineering.

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