中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (24): 4376-4380.doi: 10.3969/j.issn.1673-8225.2012.24.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

胸椎黄韧带骨化症与骨形态发生蛋白4基因单核苷酸多态性的关联

赵伟光1，刘振武1，刘 利1，谢延平1，江丽强1，林 欣2   

  1. 1邯郸市中心医院骨一科，河北省邯郸市 056001；
    2首都医科大学附属北京天坛医院骨科，北京市 100050
  • 收稿日期:2012-04-01 修回日期:2012-04-17 出版日期:2012-06-10 发布日期:2013-11-05
  • 通讯作者: 林欣，博士生导师，主任医师，首都医科大学附属北京天坛医院骨科，北京市 100050 linxin@bjtth.com
  • 作者简介:赵伟光★，1984年生，河北省邢台市人，汉族，硕士，2011年首都医科大学毕业，医师，主要从事骨科临床工作。 zhaoweiguang361@163.com
  • 基金资助:

    国家自然科学基金(30872599)；北京市自然科学基金(7092028)

Bone morphogenetic protein-4 gene polymorphism associated with ossification of the thoracic ligamentum flavum

Zhao Wei-guang1, Liu Zhen-wu1, Liu Li1, Xie Yan-ping1, Jiang Li-qiang1, Lin Xin2   

  1. 1Department of Orthopedics, Handan Central Hospital, Handan 056001, Hebei Province, China;
    2Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
  • Received:2012-04-01 Revised:2012-04-17 Online:2012-06-10 Published:2013-11-05
  • Contact: Lin Xin, Doctoral supervisor, Chief physician, Department of Orthopedics, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China linxin@bjttyy.com
  • About author:Zhao Wei-guang★, Master, Physician, Department of Orthopedics, Handan Central Hospital, Handan 056001, Hebei Province, China zhaoweiguang361@163.com

PDF

442

被引次数

13

摘要：

背景：骨形态发生蛋白4基因与胸椎黄韧带骨化症的相关性研究目前较少。
目的：测观察骨形态发生蛋白4的2个单核苷酸多态性位点rs17563和rs2855532与胸椎黄韧带骨化症的关联。
方法：胸椎黄韧带骨化症患者和正常对照者各40例，收集受试者的周围静脉血提取DNA，PCR法进行目的片段骨形态发生蛋白4的单核酸多态性位点rs17563和rs2855532的扩增并测序。
结果与结论：黄韧带骨化症组中rs17563和rs2855532位点带“T”基因型及等位基因型频率明显高于对照组(P < 0.05)。证实，骨形态发生蛋白4上的2个单核酸多态性位点rs17563和rs2855532等位基因型突变与胸椎黄韧带骨化症的发生有关。

关键词: 黄韧带骨化症, 胸椎, 骨形态发生蛋白4, 单核苷酸多态性, 聚合酶链反应, 病例-对照研究, 组织构建

Abstract:

BACKGROUND: There are few studies addressing the association of bone morphogenetic protein-4 gene and ossification of the thoracic ligamentum flavum (OLF).
OBJECTIVE: To explore whether two single nucleotide polymorphisms of the bone morphogenetic protein-4 were related to the morbidity and severity of OLF.
METHODS: The venous blood samples of 40 OLF patients and 40 non-OLF controls were collected. All experimental participants were matched in age-sex composition. The DNA extracted from the peripheral venous blood was amplified and sequenced at two sites of rs17563 and rs2855532 by using PCR.
RESULTS AND CONCLUSION: The frequency of “T” genotype and allelotype at rs17563 and rs2855532 in the OLF patients was higher than that in the non-OLF patients (P < 0.05). The two single nucleotide polymorphisms (rs17563 and rs2855532) can increase the incidence of OLF.

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