中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9950-9952.doi: 10.3969/j.issn.1673-8225.2010.53.017

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

冻干辐照猪硬脑膜的抗原性

张友来，曾元临，邹立津，辛国华   

  1. 南昌大学第一附属医院烧伤中心，江西省南昌市，330006
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 通讯作者: 曾元临，教授，主任医师，南昌大学第一附属医院烧伤中心，江西省南昌市 330006
  • 作者简介:张友来★，男，1983年生，江西省南昌市人，汉族，2009年南昌大学医学院毕业，硕士，医师，主要从事组织工程皮肤、烧伤后期康复与循证医学等研究。 youlaizhang@yahoo.cn

Antigenicity of freeze-dried irradiated pig dura mater

Zhang You-lai, Zeng Yuan-lin, Zou Li-jin, Xin Guo-hua   

  1. Burn Center, the First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Zeng Yuan-lin, Professor, Chief physician, Burn Center, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Zhang You-lai★, Master, Physician, Burn Center, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China youlaizhang@yahoo.cn

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摘要：

背景：抗原性是异种组织移植最主要的问题，是移植成功与否的关键。
目的：观察经冻干辐照处理后的猪硬脑膜抗原性变化。
方法：取新鲜猪硬脑膜，经冻干、辐照处理后分别制备成冻干猪硬脑膜、辐照猪硬脑膜和冻干辐照猪硬脑膜。分别移植于大白兔硬脑膜上，于术后3，8周行各组实验动物淋巴细胞毒实验。
结果与结论：移植冻干组和辐照组淋巴细胞死亡率比较差异无显著性意义(P > 0.05)，但均高于冻干辐照组(P < 0.05)。结果提示经冻干辐照处理后的猪硬脑膜抗原性低。

关键词: 冷冻干燥法, 辐照, 硬脑膜, 抗原性, 异种移植

Abstract:

BACKGROUND: Antigenicity is the main problem exists in allografts transplantation and decides the fate of transplantation.  
OBJECTIVE: To observe the antigen changes of freeze-dried irradiated pig dura mater.
METHODS: Freeze-dried porcine dura mater, irradiated porcine dura mater and freeze-dried and irradiated porcine dura mater were prepared by freeze-dried and (or) irradiated treatment and were transplanted into dura mater of white rabbits. Immunologic property was determined at 3 and 8 weeks after operation.
RESULTS AND CONCLUSION: There was no significant difference of lymphocytes death rates in the freeze-dried group and irradiated group (P > 0.05), both of which were greater than that of the freeze-dried irradiated group (P < 0.05). The results demonstrated that, the pig freeze-dried irradiated dura mater has low antigenicity.

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