中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8369-8372.doi: 10.3969/j.issn.1673-8225.2010.45.003

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

急性肝衰竭大鼠血清诱导骨髓间充质干细胞表达甲胎蛋白和白蛋白　

王  莹，赵文静，叶冬霞，刘淑荣   

  1. 吉林省肝胆病医院，吉林省长春市 130062
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 作者简介:王莹★，女，1975年生，吉林省长春市人，汉族，2009年北华大学毕业，硕士，副主任医师，主要从事干细胞治疗终末期肝病的临床应用研究。 xingyuewj2@yahoo.com.cn 并列第一作者：赵文静☆，女，1973年生，吉林省吉林市人，汉族，2009年吉林大学毕业，博士，副主任技师，主要从事间充质干细胞的基础研究。
  • 基金资助:

    长春市科技发展计划项目（08SF07），脐血干细胞治疗终末期肝病的临床应用研究。

Expression of alpha-fetoprotein and albumin in bone marrow mesenchymal stem cells induced by hepatic failure rat serum

Wang Ying, Zhao Wen-jing, Ye Dong-xia, Liu Shu-rong   

  1. Jilin Provincial Liver and Gallbladder Diseases Hospital, Changchun  130062, Jilin Province, China
  • Online:2010-11-05 Published:2010-11-05
  • About author:Wang Ying★, Master, Associate chief physician, Jilin Provincial Liver and Gallbladder Diseases Hospital, Changchun 130062, Jilin Province, China xingyuewjz@yahoo.com.cn Zhao Wen-jing, Doctor, Associate chief technician, Jilin Provincial Liver and Gallbladder Diseases Hospital, Changchun 130062, Jilin Province, China Wang Ying and Zhao Wen-jing contributed equally to this article.
  • Supported by:

     the Scientific and Technical Development Project of Changchun City, No. 08SF07*

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摘要：

背景：研究发现肝细胞生长因子、成纤维细胞生长因子4和表皮细胞生长因子等可诱导骨髓间充质干细胞向肝细胞分化，关于肝衰竭大鼠血清对骨髓间充质干细胞的诱导分化作用尚未见报道。
目的：观察急性肝衰竭大鼠血清对骨髓间充质干细胞的诱导分化作用。
方法：采用密度梯度离心法从SD大乳鼠股骨、胫骨分离培养骨髓间充质干细胞，体外扩增培养。采用急性肝衰竭大鼠血清诱导培养骨髓间充质干细胞，以常规培养液诱导为对照，观察细胞形态的变化，诱导培养14 d采用免疫细胞化学方法检测甲胎蛋白、白蛋白的表达。
结果与结论：急性肝衰竭大鼠血清诱导培养14 d， 可见骨髓间充质干细胞发生明显的形态学改变，镜下观察细胞变得稍大而扁平，呈类上皮样细胞，甲胎蛋白和白蛋白表达阳性率分别为(54.8±7.64)%和(45.9±9.68)%；对照组细胞未发生形态学改变，无甲胎蛋白和白蛋白的表达。证明了急性肝衰竭大鼠血清对骨髓间充质干细胞的诱导分化作用，可诱导骨髓间充质干细胞表达甲胎蛋白和白蛋白。

关键词: 骨髓, 间充质干细胞, 肝衰竭, 诱导, 干细胞

Abstract:

BACKGROUND: It was found that hepatocyte growth factor, fibroblast growth factor-4 and epidermal growth factor could induce differentiation of bone marrow mesenchymal stem cells (BMSCs) into hepatocytes. There were no reports on the inducing effect of hepatic failure rat serum on BMSCs.
OBJECTIVE: To study the effects of hepatic failure rat serum on the induced differentiation of BMSCs.
METHODS: BMSCs were isolated from SD rat femur and tibia by density gradient centrifugation and culture in vitro. BMSCs were cultured in DMEM containing the hepatic failure rat serum and conventional culture medium as control. The morphological changes of cells were observed and the expression of alpha-fetoprotein and albumin was measured by immunocytochemistry method on 14 days.
RESULTS AND CONCLUSION: Obvious morphological changes were observed on 14 day, which showed that BMSCs became slightly large and flat cells as epithelial cells. The positive rates of alpha-fetoprotein and albumin were (54.8±7.64)% and (45.9±9.68)% respectively. Cells in the control group did not have morphological changes, no alpha-fetoprotein and albumin expression. The results showed that BMSCs can be induced to express alpha-fetoprotein and albumin by hepatic failure rat serum.

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