中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (44): 8233-8236.doi: 10.3969/j.issn.1673-8225.2010.44.016

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

霍乱毒素B亚单位对树突状细胞成熟的影响

郭路生，张  佩，伦恒忠，严晓芳   

  1. 辽宁医学院免疫与微生物学教研室，辽宁省锦州市 121000　
  • 出版日期:2010-10-29 发布日期:2010-10-29
  • 通讯作者: 张佩，博士，教授，主要从事黏膜免疫的研究。辽宁医学院免疫与微生物学教研室，辽宁省锦州市 121000 jyzhangpei@163.com
  • 作者简介:郭路生★，男，1982年生，吉林省吉林市人，汉族，辽宁医学院在读硕士，主要从事黏膜免疫的研究,现工作于吉林医药学院附属医院 465kjk@163.com
  • 基金资助:

    辽宁省教育厅高等学校科学研究项目(20071174)。

Effect of cholera toxin B subunit on the maturation of dendritic cells

Guo Lu-sheng, Zhang Pei, Lun Heng-zhong, Yan Xiao-fang   

  1. Department of Immunology and Microbiology, Liaoning Medical University, Jinzhou  121000, Liaoning Province, China　
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Zhang Pei, Doctor, Professor, Department of Immunology and Microbiology, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China jyzhangpei@163.com
  • About author:Guo Lu-sheng★, Studying for master’s degree, Department of Immunology and Microbiology, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China Working at Affiliated Hospital of Jilin Medical College 465kjk@163.com
  • Supported by:

    the Scientific Research Project for Institute of Higher Education of Education Bureau of Liaoning Province, No. 20071174*

PDF

445

被引次数

8

摘要：

背景：已有实验证明，霍乱毒素可诱导树突状细胞成熟。霍乱毒素B为霍乱毒素的无毒亚单位，是一种良好的免疫佐剂和输送抗原载体，其是否能促进树突状细胞成熟至今尚未得到证实。
目的：观察树突状细胞2.4的表型和功能变化，初步探讨霍乱毒素B对树突状细胞成熟的影响。
方法：通过细胞培养增殖树突状细胞2.4。将树突状细胞分成5组：空白对照组：不进行任何处理；霍乱毒素B 0.1，1.0，10 mg/L组：分别在培养液中加入相应霍乱毒素B；阳性对照组：培养液中加入脂多糖，终浓度1 mg/L。每组设6复孔。采用流式细胞术检测培养不同时间树突状细胞2.4细胞表面CD80，CD86，MHC-Ⅱ类分子的表达，并行体外混合淋巴细胞反应，MTT法观察树突状细胞对同种T细胞的刺激能力。
结果与结论：10 mg/L霍乱毒素B显著增强树突状细胞表面分子(CD80，CD86和MHC-Ⅱ)的表达(P < 0.01)，并显著提高树突状细胞对T细胞的激活能力(P < 0.01)。阳性对照组与空白组之间差异有显著性意义(P < 0.01)。结果显示霍乱毒素B能增强树突状细胞的活化与成熟，对其免疫学功能起正性调节作用。

关键词: 霍乱毒素B, 脂多糖, 树突状细胞, 成熟, 影响

Abstract:

BACKGROUND: The existing experimental results showed that cholera toxin can induce dendritic cell maturation. Cholera toxin B is non-toxic subunit of cholera toxin, which is a good adjuvant and antigen delivery vector, whether it can promote dendritic cell maturation has not yet been confirmed.
OBJECTIVE: To observe the dendritic cell phenotype and function of 2.4 changes in the initial study of cholera toxin B on dendritic cell maturation. 
METHODS: Dendritic cell 2.4 was proliferated by cell culture. Dendritic cells were divided into five groups: control group: there was no treatment; cholera toxin B 0.1, 1.0, 10 mg/L groups: cholera toxin B with the respective final concentrations were added in the culture medium; positive control group: lipopolysaccharide, with final concentration of 1 mg/L, was added into the culture medium. There were 6 holes in each group. Expression of 2.4 cell surface CD80, CD86, MHC-Ⅱ molecule in dendritic cells cultured at different times were detected by flow cytometry, followed by parallel in vitro mixture with lymphocyte reaction. Types of dendritic cells on T cells with stimulation capacity were observed by MTT. 
RESULTS AND CONCLUSION: 10 mg/L cholera toxin B significantly enhanced dendritic cell surface molecules (CD80, CD86 and MHC-Ⅱ) expression (P < 0.01), and significantly improved the dendritic cells to T cell activation capacity (P < 0.01). There were significant differences between positive control group and blank control group (P < 0.01). Cholera toxin B can enhance the activation of dendritic cells mature, its immune function from the positive regulatory role.

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