中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (44): 8237-8240.doi: 10.3969/j.issn.1673-8225.2010.44.017

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

傣族健康成人及青少年T淋巴细胞亚群正常值调查

苏莹珍1,2，俞守义2，贾曼红1，杨  莉1，施玉华1，陈  玲1，杨朝军1，马艳玲1   

  1. 1云南省疾病预防控制中心性病艾滋病防制科，云南省昆明市 650022；2南方医科大学公共卫生与热带医学学院流行病学系， 广东省广州市 510515
  • 出版日期:2010-10-29 发布日期:2010-10-29
  • 通讯作者: 马艳玲，副科长，硕士，副主任技师，云南省疾病预防控制中心性病艾滋病防制科，云南省昆明市 650022 mayanling68@yahoo.com.cn
  • 作者简介:苏莹珍☆，女，1978年生，云南省昆明市人，南方医科大学热带公共卫生学院在读博士，技师，主要从事HIV分子流行病学研究。 suyingzhen@163.com
  • 基金资助:

    广州市科技攻关重点项目分题(2006Z1-E0091)。

Investigation of normal values of T-lymphocyte subsets in healthy Dai adolescents and adults

Su Ying-zhen 1,2 , Yu Shou-yi2, Jia Man-hong1, Yang Li1, Shi Yu-hua1, Chen Ling1, Yang Chao-jun1, Ma Yan-ling1　   

  1. 1 Center for AIDS/ATDs Prevention and Control, Yunnan Center for Disease Control and Prevention, Kunming   650022, Yunnan Province, China; 2 Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou    510515, Guangdong Province, China　
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Ma Yan-ling, Master, Associate chief technician, Center for AIDS/ATDs Prevention and Control, Yunnan Center for Disease Control and Prevention, Kunming 650022, Yunnan Province, China mayanling68@yahoo.com.cn
  • About author:Su Ying-zhen☆, Studying for doctorate, Technician, Center for AIDS/ATDs Prevention and Control, Yunnan Center for Disease Control and Prevention, Kunming 650022, Yunnan Province, China; Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, Guangdong Province, China suyingzhen@163.com
  • Supported by:

     the Branch Subject of Science and Technology Development Program of Guangzhou, No. 2006Z1-E0091*

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409

被引次数

7

摘要：

背景：健康状况下，T淋巴细胞亚群及其他免疫细胞在数量上保持相对稳定的状态，由于T淋巴细胞亚群的正常值受年龄、性别、种族等因素的影响，容易导致感染性疾病或自身免疫疾病的发生。因此建立正常参考值对判断不同疾病状态免疫功能、疾病进展以及恢复状态均具有重要意义。
目的：调查健康傣族成人及青少年外周血T淋巴细胞亚群绝对计数正常值。
方法：使用四色荧光抗体对392名健康傣族成人及青少年外周血T淋巴细胞亚群进行标记，采用FACSCalibur流式细胞仪进行检测并分析结果。
结果与结论：82名傣族健康青少年外周血T淋巴细胞亚群绝对数均值及参考范围分别为：淋巴细胞为3 054×106 (1 691× 106~4 845×106)L-1；CD3+细胞为2 055×106(1 183×106~3 280×106)L-1；CD4+细胞为1 072×106(583×106~1 666×106)L-1；CD8+细胞为825×106(381×106~1 665×106)L-1；CD4+ /CD8+值为1.38(0.44~2.36)。310名成人T淋巴细胞亚群绝对数：淋巴细胞为2 649×106(1 243×106~4 754×106)L-1；CD3+为1 763×106(828×106~3 115×106)L-1；CD4+为985×106(450×106~ 1 708×106)L-1；CD8+为663×106(275×106~1 335×106)L-1；CD4+ /CD8+值为1.64(0.74~2.00)。男性淋巴细胞数和CD8+T淋巴细胞绝对数大于女性，而CD3+百分比和CD4+百分比低于女性(P < 0.05)。除CD4+T淋巴细胞绝对数外，各年龄层健康人淋巴细胞数、T淋巴细胞绝对数、CD8+T细胞绝对数及比例均不同，尤其是青少年组CD8+T淋巴细胞绝对数和CD8+ %高于其他年龄组，且随年龄增加而降低。与中国不同地区健康人群比较，傣族健康人CD4+T淋巴细胞绝对数高于国内其他地区。此次调查建立了傣族人正常淋巴细胞、T淋巴细胞亚群的表型参考范围。

关键词: 傣族, T淋巴细胞亚群, 正常值, 参考范围, 流式细胞仪, 调查

Abstract:

BACKGROUND: Under healthy status, T-lymphocyte subsets and other immunocyte maintain stable condition in quantity. As the normal value of T-lymphocyte subsets is influenced by age, gender and race, it may induce infectious disease or autoimmune disease. Therefore, establishment of normal value is important for identifying immune function, disease development and recovery of various diseases.
OBJECTIVE: To investigate the normal value of peripheral blood T-lymphocyte subsets in healthy adolescents and adults.
METHODS: Peripheral blood was collected from 392 Dai healthy adults and adolescents and stained with monoclonal antibodies supplied by Multi TEST four colors T lymphocyte kit. The samples were tested and analyzed by FACS Calibur.
RESULTS AND CONCLUSION: The absolute counting mean of the T-lymphocyte subsets and reference ranges of 82 healthy Dai adolescent subjects were as follows: Lymphocytes 3 054×106 (1 691×106-4 845×106)/L; CD3+ cells 2 055×106 (1 183×106-  3 280×106)/L; CD4+cells 1 072×106 (583×106-1 666×106)/L; CD8+ cells 825×106 (381×106-1 665×106)/L, and the ratio of CD4+ /CD8+ was 1.38 (0.44-2.36). The absolute mean of the T-lymphocyte subsets and reference ranges of 310 healthy adults subjects were as follows: Lymphocytes 2 649×106 (1 243×106-4 754×106)/L; CD3+ 1 763×106 (828×106-3 115×106)/L; CD4+985×106 (450×106-1 708×106)/L; CD8+ 663×106 (275×106-1 335×106)/L; the ratio of CD4+ /CD8+ was 1.64 (0.74-2.00). The absolute numbers of lymphocyte subsets and CD8+ of male were significantly more than the female, while the percentage of CD3+ and CD4+of male was remarkably less than the female (P < 0.05). except of CD4+T-lymphocyte, lymphocyte number, T-lymphocyte absolute number, CD8+T cells absolute number and percentage in healthy people of each age stage were different, in particular, CD8+T cells absolute number and percentage in adolescents were greater than other age groups, and decreased with aging. Compared with healthy populations in other regions, the absolute numbers of CD4+T cells in healthy Dai minority were greater. This study established immunophenotyping of lymphocyte and T cell subsets reference ranges for healthy Dai populations.

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