中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (51): 8294-8299.doi: 10.3969/j.issn.2095-4344.2015.51.017

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

超低频电磁场处理水对骨骼肌细胞糖脂代谢及线粒体功能的影响

封卓群1，徐晓阳1，潘红英1，李 捷2，彭 元3   

  1. 1华南师范大学体育科学学院，广东省广州市 510006；2广东省体育局，广东省广州市 510056；3广东骏丰频谱股份有限公司，广东省深圳市 518028
  • 收稿日期:2015-09-24 出版日期:2015-12-10 发布日期:2015-12-10
  • 通讯作者: 徐晓阳，教授，华南师范大学体育科学学院，广东省广州市 510006
  • 作者简介:封卓群，女，1991年生，河南省平顶山市人，汉族，2015年华南师范大学体育科学学院毕业，硕士，主要从事运动生化及营养的研究。
  • 基金资助:

    国家体育总局科研课题资助项目(2014B046)；骏丰频谱水公司资助项目

Effect of spectrum water on glucolipid metabolism and mitochondrial function of skeletal muscle cells

Feng Zhuo-qun1, Xu Xiao-yang1, Pan Hong-ying1, Li Jie2, Peng Yuan3   

  1. 1School of Physical Education and Sports Science, South China Normal University, Guangzhou 510006, Guangdong Province, China; 2Sports Bureau of Guangdong Province, Guangzhou 510056, Guangdong Province, China; 3JFC Health, Shenzhen 518028, Guangdong Province, China
  • Received:2015-09-24 Online:2015-12-10 Published:2015-12-10
  • Contact: Xu Xiao-yang, Professor, School of Physical Education and Sports Science, South China Normal University, Guangzhou 510006, Guangdong Province, China
  • About author:Feng Zhuo-qun, Master, School of Physical Education and Sports Science, South China Normal University, Guangzhou 510006, Guangdong Province, China
  • Supported by:

    the Scientific Research Project of General Administration of Sport of China, No. 2014B046; a grant from JFC Health

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被引次数

2

摘要：

背景：超低频电磁场处理水(简称频谱水)作用于生物体, 在很多方面能够改善生物体的生理功能，但其对骨骼肌细胞糖脂代谢和线粒体功能的影响上，还需要更进一步的理论和实验研究。
目的：探究频谱水培养对C2C12骨骼肌细胞糖脂代谢和线粒体功能的作用。
方法：采用频谱水配制的DMEM培养C2C12骨骼肌细胞96 h后收样，甲基噻唑基四唑(MTT)细胞计数法检测细胞增殖，检测培养基中葡萄糖含量、细胞内三酰甘油浓度、线粒体ATP含量，线粒体膜电位，以及葡萄糖糖转运子1、4和细胞色素C氧化酶的蛋白水平。
结果与结论：频谱水培养C2C12骨骼肌细胞96 h细胞葡萄糖消耗量增加2.4%(P < 0.05)，三酰甘油浓度略有减少(P > 0.05)，葡萄糖糖转运子1蛋白表达增加35.8%(P < 0.05)，细胞色素C氧化酶1蛋白表达增加43.7%(P < 0.01)，线粒体ATP含量和线粒体膜电位均没有明显变化(P > 0.05)。结果提示：①频谱水培养C2C12骨骼肌细胞可通过增加骨骼肌细胞膜上葡萄糖糖转运子1的含量来提高骨骼肌细胞葡萄糖利用量。②频谱水不改变正常骨骼肌细胞线粒体膜电位。③频谱水可能通过提高线粒体中细胞色素C氧化酶1的表达量而在骨骼肌细胞能量需要增加时加强线粒体的产能功能。 

 

关键词: 组织构建, 组织工程, 超低频电磁场处理水, C2C12骨骼肌细胞, 细胞增殖活性, 细胞葡萄糖消耗量, 葡萄糖糖转运子, 三磷酸腺苷, 线粒体膜电位, 细胞色素C氧化酶

Abstract:

BACKGROUND: The ultra-low frequency electromagnetic field treated water (spectrum water) can improve the physiological function of organisms in many aspects. But its effects on glucolipid metabolism and mitochondrial function of C2C12 skeletal muscle cells need further theoretical and experimental research.
OBJECTIVE: To explore the effect of spectrum water on glucolipid metabolism and mitochondrial function of C2C12 skeletal muscle cells.
METHODS: C2C12 skeletal muscle cells were cultured in spectrum water-prepared DMEM for 96 hours. Then, 
the proliferation activity of C2C12 skeletal muscle cells, glucose content in the culture medium, intracellular triglyceride content, mitochondrial adenosine triphosphate content, mitochondrial membrane potential, protein expression of glucose transporters 1, 4 and cytochrome C oxidase were measured.
RESULTS AND CONCLUSION: Compared with the control group, the glucose consumption of C2C12 skeletal muscle cells cultured by spectrum water was increased by 2.4% (P < 0.05), the protein expression of glucose transporter 1 was increased by 35.8% (P < 0.05), and the protein expression of cytochrome C oxidase was increased by 43.7% (P < 0.01). Intracellular triglyceride, mitochondrial adenosine triphosphate content, and mitochondrial membrane potential had no significant changes (P > 0.05). These findings indicate that (1) the glucose consumption of C2C12 skeletal muscle cells cultured by spectrum water can be enhanced by the overexpression of glucose transporter 1; (2) the mitochondrial membrane potential of C2C12 skeletal muscle cells cultured by spectrum water has no significant variation; (3) the mitochondrial function of C2C12 skeletal muscle cells cultured by spectrum water can be enhanced by the overexpression of cytochrome C oxidase.
 

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