中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (42): 6832-6837.doi: 10.3969/j.issn.2095-4344.2015.42.020

• 组织构建综述 tissue construction review • 上一篇    下一篇

组织工程椎间盘纤维环：如何更接近于天然

袁德超1，2，陈 竹1，冯大雄2，冯 刚1，2   

  1. 1川北医学院第二临床医学院组织工程与干细胞研究所•南充市中心医院，四川省南充市 637000；2四川医科大学，四川省泸州市 646000
  • 出版日期:2015-10-08 发布日期:2015-10-08
  • 通讯作者: 冯刚，教授，硕士生导师，川北医学院第二临床医学院组织工程与干细胞研究所?南充市中心医院，四川省南充市 637000；四川医科大学，四川省泸州市 646000
  • 作者简介:袁德超，男，1990年生，四川省富顺县人，汉族，四川医科大学在读硕士，医师，主要从事脊柱外科研究。
  • 基金资助:

    国家自然科学基金(81171472、81201407)；四川省教育厅创新团队资助项目(13TD0030)；四川教育厅重大培育项目(15CZ0021)；南充市科技支撑计划项目(14A0021)

How to build a tissue-engineered intervertebral disc annulus fibrosus that is more close to the natural one?

Yuan De-chao1, 2, Chen Zhu1, Feng Da-xiong2, Feng Gang1, 2   

  1. 1Institute of Tissue Engineering and Stem Cells, Second Clinical School of North Sichuan Medical College, Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China; 2Sichuan Medical University, Luzhou 646000, Sichuan Province, China
  • Online:2015-10-08 Published:2015-10-08
  • Contact: Feng Gang, Professor, Master’s supervisor, Institute of Tissue Engineering and Stem Cells, Second Clinical School of North Sichuan Medical College, Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China; Sichuan Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Yuan De-chao, Studying for master’s degree, Physician, Institute of Tissue Engineering and Stem Cells, Second Clinical School of North Sichuan Medical College, Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China; Sichuan Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81171472, 81201407; the Innovation Team Program of Sichuan Provincial Education Department, No. 13TD0030; the Major Culture Project of Sichuan Provincial Education Department, No. 15CZ0021; the Scientific Support Plan of Nanchong City, No. 14A0021

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摘要：

背景：组织工程椎间盘为椎间盘退行性变疾病的生物治疗提供了新的方法，组织工程纤维环是构建组织工程椎间盘的关键之一。
目的：从纤维环结构特点、材料支架、种子细胞3方面，归纳总结组织工程纤维环的研究进展。
方法：用计算机检索PubMed、万方数据库，限定时间为2000至2015年，中英文检索词分别为“组织工程，椎间盘，纤维环，种子细胞，支架材料，构建”和“tissue engineering, intervertebral disc，annulus fibrosus， seed cell， scaffold， construction”。按纳入和排除标准对文献进行筛选，保留48篇文章进行综述。
结果与结论：早期的组织工程纤维环的研究仅侧重于支架上的细胞黏附、细胞增殖和细胞外基质的分泌，目前的组织工程纤维环可以在细胞功能、组织结构和力学特点等方面比较接近于天然纤维环，在动物实验中取得了一定的成果。但是以目前的材料和技术，要构建完全类似于天然纤维环的组织工程纤维环仍具有一定的难度，支架材料、培养条件、种子细胞的获取等诸多方面仍需要进一步完善。目前的构建策略主要集中在单相的纤维环支架上，双相纤维环及完整椎间盘支架将是研究的趋势。干细胞的诱导分化技术为组织工程纤维环提供了广阔的种子细胞来源。
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 组织构建, 组织工程, 组织工程纤维环, 支架材料, 种子细胞, 国家自然科学基金

Abstract:

BACKGROUND: Tissue-engineered intervertebral disc has provided a new biological therapeutic approach for intervertebral disc degeneration. Tissue-engineered annulus fibrosus is one key step of constructing a complete tissue-engineered intervertebral disc.
OBJECTIVE: To sum up the research progress of tissue-engineered annulus fibrosus from the following aspects: structural features, scaffold materials, seed cells.
METHODS: PubMed database and Wanfang database (2000-2015) were retrieved by the key words of “tissue engineering, intervertebral disc, annulus fibrosus, seed cell, scaffold, construction” in Chinese and in English, respectively. According to inclusion and exclusion criteria, 48 literatures were involved for summarization. 
RESULTS AND CONCLUSION: Previous studies about tissue-engineered annulus fibrosus only focused on cell adhesion, proliferation and extracellular matrix secretion on the scaffold. Currently, tissue-engineered annulus fibrosus exhibit similar features to the natural annulus fibrosus in the following aspects: cell function, tissue structure and mechanical features, and relevant animal experiments have achieved certain results in animal experiments. However, it is still difficult to build a tissue-engineered annulus fibrosus entirely similar to the natural one, and we need to further improve scaffold materials, culture conditions, collection of seed cells. The current strategies of annulus fibrosus construction still focus on single phase of scaffold, and the biphasic scaffold and complete intervertebral disc scaffold will be the trend of the researches. Technology of induced differentiation of stem cells provides a broach source of seed cells for tissue-engineered annulus fibrosus.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words:  Intervertebral Disk, Stents, Stem Cells