中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (20): 3147-3151.doi: 10.3969/j.issn.2095-4344.2015.20.006

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

组织芯片检测星形胶质细胞上调基因1与原发性肝细胞癌病理因素的相关性

李  聪1，张  巍2，刘学智3，刘博君1，张洪海1，杨耿侠1，生守鹏1，孙  玉1，袁春旺1，郑加生1   

  1. 1首都医科大学附属北京佑安医院肿瘤微创介入治疗中心，北京市  100069；2解放军总医院骨科，北京市  100853；3涞水县医院骨科，河北省保定市  074103
  • 出版日期:2015-05-14 发布日期:2015-05-14
  • 通讯作者: 郑加生，博士，主任医师，教授，首都医科大学附属北京佑安医院肿瘤微创介入治疗中心，北京市 100069
  • 作者简介:李聪，男，1985年生，山东省人，汉族，2014年哈尔滨医科大学毕业，博士，医师，主要从事腹部实体肿瘤的临床和基础研究。
  • 基金资助:

    国家自然科学基金面上项目(81472328)；佑安肝病艾滋病基金(BJYAH-2011-034)； 国家科技支撑项目(2012BAI15B08)，国家传染病重大专项(2012ZX10002015-002)

Tissue microarray detection for astrocyte elevated gene-1 and its correlation with pathological factors in primary hepatocellular carcinoma 

Li Cong1, Zhang Wei2, Liu Xue-zhi3, Liu Bo-jun1, Zhang Hong-hai1, Yang Geng-xia1, Sheng Shou-peng1, Sun Yu1, Yuan Chun-wang1, Zheng Jia-sheng1   

  1. 1Center of Oncology and Minimally Invasive Intervention, Beijing YouAn Hospital, Capital Medical University, Beijing 100069, China; 2Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China; 3Department of Orthopedics, Laishui County Hospital, Baoding 071002, Hebei Province, China
  • Online:2015-05-14 Published:2015-05-14
  • Contact: Zheng Jia-sheng, M.D., Chief physician, Professor, Center of Oncology and Minimally Invasive Intervention, Beijing YouAn Hospital, Capital Medical University, Beijing 100069, China
  • About author:李聪，男，1985年生，山东省人，汉族，2014年哈尔滨医科大学毕业，博士，医师，主要从事腹部实体肿瘤的临床和基础研究。
  • Supported by:

    the National Natural Science Foundation of China, No. 81472328; YouAN Fund for Liver Diseases and AIDS, No. BJYAH-2011-034; the National Science and Technology Support Program, No. 2012BAI15B08; the National Special Fund for Infectious Diseases, No. 2012ZX10002015-002

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摘要：

背景：星形胶质细胞上调基因1是肿瘤发生转移早期的重要标记分子之一，很有可能成为肝癌临床治疗的分子靶标。
目的：探索原发性肝细胞癌患者星形胶质细胞上调基因1在其癌组织中的表达水平，并推测其在原发性肝细胞癌患者微创疗效中的地位。
方法：采用组织芯片方法和免疫组织化学染色方法检测120例原发性肝细胞癌患者肿瘤组织中星形胶质细胞上调基因1蛋白表达水平，并与其临床病理资料进行相关性分析。
结果与结论：原发性肝细胞癌患者星形胶质细胞上调基因1蛋白表达水平与其临床分期和组织分化程度密切相关，且其星形胶质细胞上调基因1蛋白高表达水平与Ki-67阳性率和肿瘤微血管密度有关，但该蛋白高表达与患者的性别及年龄无相关性。说明星形胶质细胞上调基因1蛋白表达水平与常规恶性检测指标相关，检测该蛋白表达水平对于判断原发性肝细胞癌具有重要意义。

关键词: 组织构建, 组织工程, 星形胶质细胞上调基因1, 组织芯片, 肝细胞癌, 免疫组化, Ki-67, 临床病理, 国家自然科学基金

Abstract:

BACKGROUND: Astrocytes elevated gene-1 is an important molecular marker for early tumor metastasis, which is likely to become the molecular target in the clinical treatment of hepatocellular carcinoma.
OBJECTIVE: To investigate the astrocyte elevated gene-1 expression level in the tumor tissues of patients with hepatocellular carcinoma, and to predict its role in the minimally invasive therapy.
METHODS: Tissue microarray and immunohistochemistry methods were applied to detect astrocyte elevated gene-1 protein expression level in the tumor tissues of patients with hepatocellular carcinoma, and their clinical-pathological data were collected for correlation analysis with the astrocyte elevated gene-1 protein expression level.
RESULTS AND CONCLUSION: The protein expression level of astrocyte elevated gene-1 was closely related to clinical stage and tissue differentiation degree in patients with hepatocellular carcinoma. In addition, the high expression of astrocyte elevated gene-1 protein was correlated with the positive rate of Ki-67 and tumor micro-vessel density. However, the protein expression had no correlation with gender and age in these patients. 
Taken together, the protein expression level of astrocyte elevated gene-1 is correlated with conventional malignant indexes, which is of great significance for the judgment of hepatocellular carcinoma.

Key words: Carcinoma, Hepatocellular, Microchip Analytical Procedures, Ki-67 Antigen, Carcinoma

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