中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (47): 7615-7620.doi: 10.3969/j.issn.2095-4344.2014.47.013

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米羟基磷灰石/细菌纤维素复合组织工程支架的细胞毒性和生物相容性

王立新1，袁  峰2，万怡灶3，何啸波2   

  1. 1南通大学附属海安医院骨科，江苏省南通市  226600
    2徐州医学院附属医院骨科，江苏省徐州市  221002
    3天津大学材料学院，天津市  300000
  • 修回日期:2014-11-03 出版日期:2014-11-19 发布日期:2014-11-19
  • 通讯作者: 袁峰，主任医师，副教授，徐州医学院附属医院骨科，江苏省徐州市 221002
  • 作者简介:王立新，男，1986年生，江苏省海安县人，汉族，2012年徐州医学院毕业，硕士，医师，主要从事脊柱外科研究。
  • 基金资助:

    江苏省“科教兴卫”医学重点人才项目(H201129)

Cytotoxicity and biocompatibility of nano-hydroxyapatite/bacterial cellulose composite scaffolds

Wang Li-xin1, Yuan Feng2, Wan Yi-zao3, He Xiao-bo2   

  1. 1Department of Orthopaedics, Affiliated Haian Hospital of Nantong University, Nantong 226600, Jiangsu Province, China
    2Department of Orthopaedics, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
    3School of Materials Science of Tianjin University, Tianjin 300000, China
  • Revised:2014-11-03 Online:2014-11-19 Published:2014-11-19
  • Contact: Yuan Feng, Chief physician, Associate professor, Department of Orthopaedics, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China
  • About author:Wang Li-xin, Master, Physician, Department of Orthopaedics, Affiliated Haian Hospital of Nantong University, Nantong 226600, Jiangsu Province, China
  • Supported by:

    Jiangsu Province’s Key Provincial Talents Program, No. H201129

PDF

468

被引次数

11

摘要：

背景：天津大学材料学院利用仿生学方法制备的纳米羟基磷灰石/细菌纤维素复合支架材料，具有与天然骨相似的结构和性能。
目的：研究纳米羟基磷灰石/细菌纤维素复合组织工程支架的细胞毒性和生物相容性。
方法：①急性全身性毒性实验：将纳米羟基磷灰石/细菌纤维素材料浸提液与生理盐水分别注射至昆明小鼠腹腔，注射24，48，72 h记录小鼠体质量。②致敏实验：在日本大耳白兔背部皮下分别注射纳米羟基磷灰石/细菌纤维素材料浸提液与生理盐水，72 h内观察注射部位水肿及红斑情况，间隔14 d后再次行激发实验。③热源实验：在日本大耳白兔耳缘静脉注射纳米羟基磷灰石/细菌纤维素材料浸提液，注射后检测体温变化。④溶血实验：在稀释的兔抗凝血中分别加入纳米羟基磷灰石/细菌纤维素材料浸提液、生理盐水与蒸馏水。⑤将第3代兔骨髓间充质干细胞与纳米羟基磷灰石/细菌纤维素材料共培养，观察材料表面细胞增殖、生长及黏附状态。
结果与结论：纳米羟基磷灰石/细菌纤维素复合支架材料无急性全身毒性、无致敏性、无热源反应、无溶血反应，该支架材料具有三维网络结构，骨髓间充质干细胞在材料表面生长、增殖及黏附良好，表明纳米羟基磷灰石/细菌纤维素复合支架材料具有良好的生物相容性与细胞相容性。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 纳米羟基磷灰石/细菌纤维素, 组织工程, 生物相容性

Abstract:

BACKGROUND: Nano-hydroxyapatite/bacterial cellulose (nHA/BC) composite scaffolds prepared by nano-bionics method exhibit a structure and performance similar to natural bone.
OBJECTIVE: To study the cytotoxicity and biocompatibility of nHA/BC composite scaffolds.
METHODS: (1) Acute systemic toxicity test: nHA/BC leach liquor and normal saline were respectively injected intraperitoneally into Kunming mice. After 24, 48, 72 hours, the body mass of mice was recorded. (2) Sensitization test: nHA/BC leach liquor and normal saline were respectively injected subcutaneously into the back of Japanese white rabbits. With 72 hours of injection, edema and erythema were observed, and the experiment was done again at an interval of 14 days. (3) Pyrogen test: nHA/BC leach liquor and normal saline were respectively injected into the ear vein of Japanese white rabbits, and after injection, the body temperature of rabbits were determined. (4) Hemolysis test: Diluted rabbit anti-coagulant was added into nHA/BC leach liquor, normal saline and distilled water, respectively. (5) Passage 3 bone marrow mesenchymal stem cells from rabbits were co-cultured with nHA/BC materials, and then, cell proliferation, growth and adhesion on the material surface were observed.
RESULTS AND CONCLUSION: nHA/BC composite scaffold was of no acute systemic toxicity, non-allergenic, no pyrogenic and hemolytic reactions; nHA/BC had a three-dimensional porous structure that was better for growth, proliferation and adhesion of rabbit bone marrow mesenchymal stem cells. These findings indicate that the nHA/BC composite scaffold has good biocompatibility and cytocompatibility.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: nanoparticles, hydroxyapatites, cellulose

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