中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (29): 5261-5268.doi: 10.3969/j.issn.2095-4344.2013.29.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

脂肪间充质干细胞复合骨诱导性磷酸钙陶瓷支架的异位成骨

姚金凤1,张筱薇1,周 琦1,郑苍尚1,梁志刚1,包崇云2   

  1. 1深圳市第二人民医院口腔科,广东省深圳市  518035;2四川大学华西口腔医学院,口腔疾病研究国家重点实验室,四川省成都市  610041
  • 收稿日期:2013-05-21 修回日期:2013-06-18 出版日期:2013-07-22 发布日期:2013-07-22
  • 通讯作者: 姚金凤,主治医师,深圳市第二人民医院口腔科,广东省深圳市 518035
  • 作者简介:姚金凤☆,女,1981年生,甘肃省兰州市人,汉族,2009年四川大学华西口腔医学院毕业,博士,主治医师,主要从事口腔种植材料及骨组织工程研究。 jinfeng-yao@163.com
  • 基金资助:

    深圳市科技创新委项目(JCYJ20120830113244696),深圳市科技创新委项目(JCYJ20130401111426275)

Ectopic bone formation in adipose-derived mesenchymal stem cell-seeded osteoinductive calcium phosphate scaffolds

Yao Jin-feng1, Zhang Xiao-wei1, Zhou Qi1, Zheng Cang-shang1, Liang Zhi-gang1, Bao Chong-yun2   

  1. 1Department of Stomatology, Second People’s Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China; 2State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2013-05-21 Revised:2013-06-18 Online:2013-07-22 Published:2013-07-22
  • Contact: Yao Jin-feng, Department of Stomatology, Second People’s Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China
  • About author:Yao Jin-feng☆, M.D., Attending physician, Department of Stomatology, Second People’s Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China jinfeng-yao@163.com
  • Supported by:

    Shenzhen Science and Technology Innovation Committee, No. JCYJ20120830113244696*; Shenzhen Science and Technology Innovation Committee, No. JCYJ20130401111426275*

摘要:

背景:生物材料的骨诱导现象已经在多种动物实验中被证实。
目的:考察磷酸钙陶瓷自身固有的诱导骨生成能力在其作为骨组织工程支架时的表现。
方法:取健康家犬10只,在每只的背部肌肉内分别植入骨诱导性磷酸钙陶瓷与自体脂肪间充质干细胞复合物、非骨诱导性磷酸钙陶瓷与自体脂肪间充质干细胞复合物、骨诱导性磷酸钙陶瓷及非骨诱导性磷酸钙陶瓷,植入后8,12周,取出植入材料及其周围组织进行Micro-CT检测和组织形态学检测,评价成骨情况。
结果与结论:组织学观察结果显示,骨诱导性磷酸钙陶瓷组及骨诱导性磷酸钙陶瓷与自体脂肪间充质干细胞复合物组均有有异位骨生成,并且骨诱导性磷酸钙陶瓷与自体脂肪间充质干细胞复合物组的成骨量显著大于骨诱导性磷酸钙陶瓷组(P < 0.05);其余两组均无异位成骨。Micro-CT检测结果与组织形态学检测结果一致。结果表明骨诱导性磷酸钙陶瓷作为骨组织工程支架材料有明显的成骨优势,而脂肪间充质干细胞作为种子细胞对异位成骨有明显的促进作用。

关键词: 生物材料, 组织工程骨材料, 骨组织工程, 脂肪间充质干细胞, 骨诱导性磷酸钙陶瓷, 异位骨生成, Micro-CT

Abstract:

BACKGROUND: The phenomenon of osteoinduction by biomaterials has been proven in animal experiments.
OBJECTIVE: To investigate whether the ability of a biomaterial to initiate bone formation in ectopic implantation sites improves the performance of osteoinductive biomaterial as a scaffold for tissue-engineered bone.
METHODS: We compared ectopic bone formation by combining autologous adipose-derived stromal cells with an osteoinductive and a nonosteoinductive biphasic calcium phosphate ceramic to create a tissue engineering construction in the muscle of dogs. All implants were implanted in the back muscle of 10 adult dogs for 8 weeks and 12 weeks, including osteoinductive biphasic calcium phosphate ceramic+ adipose-derived stromal cells (osteoinductive complex group), osteoinductive biphasic calcium phosphate ceramic (osteoinductive broup), nonosteoinductive biphasic calcium phosphate ceramic+adipose-derived stromal cells (nonosteoinductive complex group), and nonosteoinductive biphasic calcium phosphate ceramic (nonosteoinductive group). Micro-CT analysis and histomorphometry were performed to evaluate and quantify ectopic bone formation.
RESULTS AND CONCLUSION: Ectopic bone formation was visible in the osteoinductive complex group and osteoinductive group, and the former group was superior to the latter one in quality of new bone (P < 0.05). However, there was no ectopic bone formation in the other two groups. Micro-CT results were consistent with the histomorphological detection. These findings indicate that osteoinductive biphasic calcium phosphate ceramic, as a kind of bone tissue engineering scaffold material, has a better osteogenic capacity, while adipose-derived mesenchymal stem cells serve as seed cells to promote the ectopic bone formation.

Key words: biomaterials, tissue-engineered bone materials, bone tissue engineering, adipose-derived mesenchymal stem cells, osteoinductive biphasic calcium phosphate ceramic, ectopic bone formation, Micro-CT

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