中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8629-8635.doi: 10.3969/j.issn.2095-4344.2012.46.016

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

病理性瘢痕中胰岛素样生长因子1及受体mRNA的表达

蔡 浩1, 2，唐 冰1，朱家源1，朱 斌1，毕良宽1   

  1. 1中山大学附属第一医院烧伤外科，广东省广州市 51008
    2广东省惠东县人民医院外三科，广东省惠东县 516300
  • 收稿日期:2012-08-11 修回日期:2012-10-23 出版日期:2012-11-11 发布日期:2012-11-11
  • 通讯作者: 朱家源，博士，主任医师，博士生导师，教授，中山大学附属第一医院烧伤外科，广东省广州市 510080 zhujiayuan@163.net
  • 作者简介:蔡浩★，男，1976年生，广东省惠东县人，汉族，2009年中山大学毕业，硕士，副主任医师，主要从事烧伤整形及慢性创面的研究。 dayu1995@sina.com 并列第一作者：朱家源，博士，主任医师，博士生导师，教授，中山大学附属第一医院烧伤外科，广东省广州市 510080 zhujiayuan@163.net

mRNA expression of insulin-like growth factor-1 and its receptor in pathological scars

Cai Hao1, 2, Tang Bing1, Zhu Jia-yuag1, Zhu Bin1, Bi Liang-kuan1   

  1. 1Department of Burn Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 5180080, Guangdong Province, China
    2Third Department of General Surgery, People’s Hospital of Huidong County, Huidong 526300, Guangdong Province, China
  • Received:2012-08-11 Revised:2012-10-23 Online:2012-11-11 Published:2012-11-11
  • Contact: Zhu Jia-yuan, Doctor, Chief physician, Doctoral supervisor, Professor, Department of Burn Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 5180080, Guangdong Province, China zhujiayuan@163.net
  • About author:Cai Hao★, Master, Associate chief physician, Department of Burn Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 5180080, Guangdong Province, China; Third Department of General Surgery, People’s Hospital of Huidong County, Huidong 526300, Guangdong Province, China dayu1995@sina.com Zhu Jia-yuan, Doctor, Chief physician, Doctoral supervisor, Professor, Department of Burn Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 5180080, Guangdong Province, China zhujiayuan@163.net Cai Hao and Zhu Jia-yuan contributed equally to this study.

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437

被引次数

3

摘要：

背景：多种肿瘤中胰岛素样生长因子1受体及其配体表达异常，且胰岛素样生长因子1是多种细胞的有丝分裂原和抗细胞凋亡的因子，控制多种细胞的增殖、分化和凋亡的进程。
目的：观察胰岛素样生长因子1及胰岛素样生长因子1受体在病理性瘢痕中的表达。
方法：收集中山大学附属第一医院烧伤外科收治患者增生性瘢痕、瘢痕疙瘩及其正常皮肤等手术切除标本，荧光定量实时PCR检测不同组织中胰岛素样生长因子1及其受体mRNA的表达水平。
结果与结论：瘢痕疙瘩及增生性瘢痕中胰岛素样生长因子1 mRNA表达水平均高于正常皮肤(P < 0.05)，且在瘢痕疙瘩及增生性瘢痕组织中胰岛素样生长因子1表达水平差异无显著性意义(P > 0.05)，胰岛素样生长因子1受体在增生性瘢痕表达水平与正常皮肤接近(P > 0.05)；而瘢痕疙瘩中胰岛素样生长因子1受体明显高于瘢痕周围正常皮肤(P < 0.05)。说明胰岛素样生长因子1及其受体在病理性瘢痕的形成过程中起重要作用。

关键词: 瘢痕疙瘩, 增生性瘢痕, 胰岛素样生长因子1受体, 胰岛素样生长因子1, 定时荧光定量PCR, 组织构建, 组织工程

Abstract:

BACKGROUND: In many tumors, insulin-like growth factor 1 and its receptor express abnormally. Insulin-like growth factor is the mitogen and anti-apoptotic factor of a variety of cells, controlling the proliferation, differentiation and apoptosis of various cells.
OBJECTIVE: To observe the expression of insulin-like growth factor-1 and insulin-like growth factor -1 receptor in pathological scar.
METHODS: Surgical specimens of hypertrophic scars, keloids and normal skin were collected from the patients admitted in the Department of Burn Surgery, First Affiliated Hospital of Sun Yat-sen University. Fluorescent quantitative real-time PCR technique was used to detect the mRNA expression of insulin-like growth factor-1 and insulin-like growth factor -1 receptor in different tissues.
RESULTS AND CONCLUSION: The mRNA expression of insulin-like growth factor-1 was higher in the hypertrophic scars and keloids than in the normal skin (P < 0.05). However, there was no difference in the mRNA expression of insulin-like growth factor-1 between the hypertrophic scars and keloids (P > 0.05). The expression of insulin-like growth factor-1 receptor in the hypertrophic scars was close to that in the normal skin (P > 0.05); while, the expression of insulin-like growth factor-1 receptor in the keloids was significantly higher than that in the surrounding tissues of keloids (P < 0.05). These findings indi

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