中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (45): 8374-8379.doi: 10.3969/j.issn.2095-4344.2012.45.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

人骨髓间充质干细胞培养上清与肝星状细胞相关酶的分泌

王 珊，张立婷，陈 红   

  1. 兰州大学第一医院，甘肃省兰州市 730030
  • 收稿日期:2012-01-03 修回日期:2012-02-29 出版日期:2012-11-04 发布日期:2012-11-04
  • 通讯作者: 陈红，硕士生导师，兰州大学第一医院，甘肃省兰州市 730030 chenhong1956@126.com
  • 作者简介:王珊★，女，1984年生，甘肃省兰州市人，汉族，兰州大学在读硕士，主要从事自体干细胞移植治疗肝纤维化的研究。 sunshine.84@163.com

Effect of human bone marrow mesenchymal stem cells culture supernatant on related enzyme expression in hepatic stellate cells

Wang Shan, Zhang Li-ting, Chen Hong   

  1. The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China
  • Received:2012-01-03 Revised:2012-02-29 Online:2012-11-04 Published:2012-11-04
  • Contact: Chen Hong, Master’s supervisor, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China chenhong1956@126.com
  • About author:Wang Shan★, Studying for master’s degree, the First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China sunshine.84@163.com

PDF

459

被引次数

8

摘要：

背景：研究证实骨髓间充质干细胞移植治疗能改善甚至逆转肝纤维化，但其具体机制尚不清楚。
目的：检测人骨髓间充质干细胞培养上清对肝星状细胞中基质金属蛋白酶2及组织抑制基质金属蛋白酶1表达的影响。
方法：采用密度梯度离心法分离人骨髓间充质干细胞，收集原代培养7 d的骨髓间充质干细胞培养上清，加入肝星状细胞中培养作为实验组，并设置单独肝星状细胞组、单独骨髓间充质干细胞组为对照。培养24，48 h后，检测各组细胞上清中基质金属蛋白酶2蛋白与组织抑制基质金属蛋白酶1蛋白的表达。
结果与结论：与单独肝星状细胞组、单独骨髓间充质干细胞组比较，实验组培养24，48 h后肝星状细胞基质金属蛋白酶2及组织抑制基质金属蛋白酶1表达减少(P < 0.05或P < 0.01)。表明骨髓间充质干细胞培养上清抑制肝星状细胞中基质金属蛋白酶2、组织抑制基质金属蛋白酶1的表达。

关键词: 肝星状细胞, 骨髓间充质干细胞, 肝纤维化, 基质金属蛋白酶2, 组织抑制基质金属蛋白酶1

Abstract:

BACKGROUND: The studies have proved that bone marrow mesenchymal stem cells transplantation therapy can improve or even reverse the liver fibrosis, but its mechanism is still unclear.
OBJECTIVE: To detect the effect of human bone marrow mesenchymal stem cells culture supernatant on the expression of matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 1 in hepatic stellate cells.
METHODS: The density gradient centrifugation was used to isolate human bone marrow mesenchymal stem cells and collected the culture supernatant of human bone marrow mesenchymal stem cells after cultured for 7 days, the human bone marrow mesenchymal stem cells culture supernatant added with the hepatic stellate cells was considered as experimental group, a simple hepatic stellate cells group and simple human bone marrow mesenchymal stem cells group were set up as control groups. The expressions of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the culture supernatant of three groups were detected after cultured for 24 and 48 hours.
RESULTS AND CONCLUSION: The expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in the experimental group was decreased when compared with simple hepatic stellate cells group and simple human bone marnow mesenchymal stem cells group (P < 0.05 or P < 0.01). The results showed that bone marrow mesenchymal stem cells culture supernatant can inhibit the expression of matrix metalloproteinase-2 protein and tissue inhibitor of matrix metalloproteinase-1 in hepatic stellate cells.

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