中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (38): 7140-7144.doi: 10.3969/j.issn.2095-4344.2012.38.023

• 药物控释材料 drug delivery materials • 上一篇    下一篇

用于组织工程医疗产品海藻酸钠主要性能指标的测定

孙 雪，奚廷斐，卢大伟   

  1. 中国食品药品检定研究院，北京市 100050
  • 收稿日期:2012-05-22 修回日期:2012-06-24 出版日期:2012-09-16 发布日期:2012-09-16
  • 作者简介:孙雪★，女，1980年生，北京市人，汉族，2006年中国药品生物制品检定所(现中国食品药品检定研究院)毕业，硕士，助理研究员，主要从事含药医疗器械的化学性能评价、组织工程、生物材料的性能评价研究。 sunxue1222@ sina.com

Sodium alginate serves as an important indicator for tissue-engineered medical products

Sun Xue, Xi Ting-fei, Lu Da-wei   

  1. National Institutes for Food and Drug Control, Beijing 100050, China
  • Received:2012-05-22 Revised:2012-06-24 Online:2012-09-16 Published:2012-09-16
  • About author:Sun Xue★, Master, Assistant researcher, National Institutes for Food and Drug Control, Beijing 100050, China sunxue1222@ sina.com

PDF

712

被引次数

8

摘要：

背景：海藻酸钠的平均分子质量及其分子质量分布，以及化学组成和序列结构的不同将会导致海藻酸钠最终用途的不同。
目的：测定用于组织工程医疗产品海藻酸钠的平均分子质量及分子质量分布，以及化学组成和序列结构。
方法：采用高效液相凝胶渗透色谱联合多角度激光散射测定仪法测定海藻酸钠的平均分子质量及分子质量分布，并通过高分辨1H-核磁共振光谱法测定其化学组成和序列结构。
结果与结论：组织工程医疗产品海藻酸钠样品的数均分子质量为7.360×104，重均分子质量为1.031×105，分子质量分布为1.401，G单体含量为0.405，M单体含量为0.595，M/G为1.47，连续M单体的平均数量为2.78，连续G单体的平均数量为1.89，连续G单体数量大于1的数值为2.72。

关键词: 海藻酸钠, 组织工程, 多角度激光散射, 核磁共振, 平均分子质量, 序列结构

Abstract:

BACKGROUND: Different average molecular weight and molecular weight distribution, as well as different chemical composition and sequence structure of sodium alginate will results in different applications of sodium alginate.
OBJECTIVE: To measure the average molecular weight and molecular weight distribution, as well as different chemical composition and sequence structure of sodium alginate used for tissue-engineered medical products.
METHODS: The average molecular weight and the molecular weight distribution were detected by size exclusion chromatography-multi-angle laser light scattering. The chemical composition and sequence structure were detected by Hydrogen-1 nuclear magnetic resonance.
RESULTS AND CONCLUSION: The sample’s number-average molecular weight was 7.360×104, weight-average molecular weight was 1.031×105 and the molecular weight distribution (Mw/Mn) was 1.401. Fg was 0.405, Fm was 0.595, the M/G was 1.47, Nm was 2.78, Ng was 1.89 and Ng>1 was 2.72.

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