中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (10): 1611-1617.doi: 10.3969/j.issn.2095-4344.0724

• 生物材料综述 biomaterial review • 上一篇    下一篇

3D生物打印在组织/器官类似物制造领域的应用

王雪欣1,张明谏2,李小兵2,韩维鑫3,李金桥1
  

  1. 1天津医科大学一中心临床学院,天津市  300070;2天津市第一中心医院整形与烧伤外科,天津市  300192;3天津市第三中心医院美容整形科,天津市  300170
  • 收稿日期:2017-11-26 出版日期:2018-04-08 发布日期:2018-04-08
  • 通讯作者: 张明谏,教授,主任医师,硕士生导师,天津市第一中心医院整形与烧伤外科,天津市 300192
  • 作者简介:王雪欣,女,1991年生,河北省武安市人,汉族,天津医科大学在读硕士,主要从事整形与烧伤研究。

Three-dimensional bioprinting of tissue/organ analogues: a review on techniques, materials and processes

Wang Xue-xin1, Zhang Ming-jian2, Li Xiao-bing2, Han Wei-xin3, Li Jin-qiao1
  

  1. 1Department of Plastic and Burn Surgery, the Clinical School of Tianjin Medical University, Tianjin 300070, China; 2Department of Plastic and Burn Surgery, Tianjin First Central Hospital, Tianjin 300192, China; 3Department of Plastic and Burn Surgery, Tianjin Third Central Hospital, Tianjin 300170, China
  • Received:2017-11-26 Online:2018-04-08 Published:2018-04-08
  • Contact: Zhang Ming-jian, Professor, Chief physician, Master’s supervisor, Department of Plastic and Burn Surgery, Tianjin First Central Hospital, Tianjin 300192, China
  • About author:Wang Xue-xin, Master candidate, Department of Plastic and Burn Surgery, the Clinical School of Tianjin Medical University, Tianjin 300070, China

摘要:

文章快速阅读:

 

文题释义:
3D生物打印:是一项在计算机辅助设计下精确分配装载活细胞的生物墨并建造具有复杂功能的活组织或人工器官的添加剂制造技术。
组织/器官类似物:与人类或动物实际组织/器官形状一致、生物学行为相似的简单或微型组织/器官结构。
喷墨技术:是常用的3D组织/器官打印技术,在计算机的控制下,通过热或电形成生物墨滴,从喷墨头的喷嘴以微升为单位非接触式逐出,并精确印刷到水凝胶基质或培养皿的特定位置。
 
摘要
背景:3D生物打印技术在组织工程领域具有巨大潜力,有望利用活细胞和活性生物材料构建具有良好生物组织相容性和生物学功能的简单组织和器官。
目的:分析3D生物打印技术和各种生物材料的特点,探讨其在相关组织/器官类似物制造中的应用。
方法:应用计算机检索1998至2017年PubMed数据库、Web of Science数据库、Medline数据库、万方医学网数据库有关3D生物打印技术和应用的相关文献。以“3D生物打印,3D生物打印技术,生物材料,组织工程”为中文主题检索词;以“3D bioprinting,3D bioprinting technology,biomaterial,tissue engineering”为英文主题检索词,检索题目和摘要同时包含上述检索词的文献共88篇,排除重复性研究和无关研究,最终纳入47 篇文献进一步分析。
结果与结论:目前应用比较广泛的3D生物打印技术主要包括喷墨式技术(热喷墨与压电喷墨)、压力辅助打印技术、激光辅助打印技术、光固化立体印刷技术(单光子和双光子)。生物墨的构成除了原生活细胞,还包括天然聚合物和合成聚合物,各有优劣势。3D生物打印含有活细胞的组织/器官类似物在组织工程领域显示出巨大的潜力,尽管已有较广泛的研究,但目前的印刷技术只能完成相对简单的组织结构和生物功能,含有活细胞的3D组织/器官生物打印仍然处于探索阶段。

关键词: 3D生物打印, 3D生物打印技术, 生物材料, 组织/器官类似物, 组织工程

Abstract:

BACKGROUND: Three-dimensional (3D) bioprinting technology has a huge potential in the tissue engineering field, which is expected to create simple tissue/organ analogues with good biological histocompatibility and biological functions by using living cells and biomaterials.
OBJECTIVE: To analyze the characteristics of 3D bioprinting technology and all kinds of biomaterials, and to explore its application in the preparation of tissues/organs analogues.
METHODS: Relevant articles published from 1998 to 2017 were searched in PubMed, Web of Science, MEDLINE, and WanFang databases. The keywords were “3D bioprinting, 3D bioprinting technology, biomaterial, tissue engineering” in English and Chinese, respectively. A total of 88 articles were initially searched and 47 eligible articles were finally reviewed in accordance with the inclusion and exclusion criteria.
RESULTS AND CONCLUSION: 3D bioprinting techniques mainly include inkjet technique (thermal inkjet and piezoelectric inkjet), pressure-assisted technique, laser-assisted technique, and stereolithography technique (single-photon-based and two-photon-based). The bio-ink consists of living cells, natural polymers and synthetic polymers. 3D bioprinting has exhibited a huge potential in the manufacture of living cell-containing tissue/organ analogues. Despite the fact that it has been widely studied, currently used 3D bioprinting techniques can only be used to prepare relatively simple structures with simple biological functions. Research on the specific tissue/organ analogues with living cells are still in its infancy.

Key words: Computer-Aided Design, Review, Tissue Engineering

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